High malt syrup and method for improving acarbose fermentation unit by using high malt syrup

A technology of high maltose syrup and high maltose syrup, which is applied in the field of medicine to achieve the effect of improving the biosynthesis and metabolism of acarbose, improving the fermentation index, and improving the fermentation level

Active Publication Date: 2013-03-20
HEBEI HUARONG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Because in large-scale industrial production, high maltose syrup and glucose are generally used as carbon sources in the fermentation production of acarbose, and high maltose syrup is processed by adding different types of enzymes to starch milk. It leads to changes in various components in high maltose syrup. High maltose syrup generally contains maltose, glucose, trisaccharides, tetrasaccharides and sugars above tetrasaccharides. Therefore, its compound components and the ratio of each component have a great influence on the growth of acarbose-producing bacteria. It has a direct effect on the synthesis and metabolism of acarbose. At present, there is no report on the detailed test or production of the carbon source of acarbose fermentation, the effect of the ratio of trisaccharides, tetrasaccharides and more than four sugars on the yield of acarbose fermentation data, this is a blank

Method used

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  • High malt syrup and method for improving acarbose fermentation unit by using high malt syrup
  • High malt syrup and method for improving acarbose fermentation unit by using high malt syrup
  • High malt syrup and method for improving acarbose fermentation unit by using high malt syrup

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Orthogonal experiments were used to explore the appropriate proportion of high maltose syrup components fermented by acarbose actinomycetes, and a suitable proportion of high maltose syrup trisaccharides, tetrasaccharides and more than tetrasaccharides was obtained.

[0022] The step that described orthogonal experiment optimizes the high maltose syrup component ratio in the fermentation medium of acarbose actinomycetes is:

[0023] (1) Prepare high maltose syrup containing trisaccharides, tetrasaccharides and more than four sugars in different proportions and with a glucose content of 0. Use different proportions of syrup and pure maltose to prepare high maltose syrup with a trisaccharide ratio of 3-21%, tetrasaccharide and tetrasaccharide ratios of 3-21%, and make trisaccharide, tetrasaccharide and tetrasaccharide in high maltose syrup Orthogonal experiment on the influence of components above sugar on the fermentation unit (the ratio of sugar is the chromat...

Embodiment 2

[0030] Example 2 Utilizing high maltose syrup to increase the fermentation unit of acarbose

[0031] Use different high maltose syrups to verify the fermenter (the volume of the seed tank is 1000L, the volume of the fermenter is 6200L, and the feeding amount is proportional to the formula)

[0032] 3. Composition of high maltose syrup:

[0033]

[0034] ; 3 groups of parallel experiment results:

[0035] Numbering fermentation unit 1 4258 2 4223 3 4290 average 4257

[0036] 2. Composition of high maltose syrup:

[0037]

[0038] ; 3 groups of parallel experiment results:

[0039] Numbering fermentation unit 1 4345 2 4332 3 4325 average 4334

[0040] 3. Composition of high maltose syrup:

[0041]

[0042] ; 3 groups of parallel experiment results:

[0043] Numbering fermentation unit 1 3879 2 3869 3 3871 average 3873

[0044] 4. Composition of high maltose...

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Abstract

The invention discloses high malt syrup and a method for improving an acarbose fermentation unit by using the high malt syrup. The high malt syrup comprises malt sugar, trisaccharide, tetrasccharide and components more than the tetrasccharide, wherein the content of glucose in the high malt syrup is 0. According to the method, the high malt syrup is utilized to be cultured in a culture medium; the culture medium is an inclined surface culture medium, a mother bottle seed culture medium or a fermentation bottle culture medium. According to the invention, the trisaccharide, tetrasccharide and components more than the tetrasccharide are adjusted, so that the fermentation level is effectively improved, the defect that thallus biosynthesis is inhibited due to improper concentration of carbon source components is overcome, the biosynthesis and the metabolism are facilitated, and the fermentation index is improved.

Description

Technical field [0001] The present invention involves a method of high malt syrup and the use of its method of improving Akaba sugar fermentation units. It is a pharmaceutical technology field. Background technique [0002] Akapo candy is a secondary metabolites produced by Actinoplanes SP.Because it has the activity of inhibiting Q-glucosidase, it can reduce the decomposition of polysaccharides and oligosaccharides in the human intestine, and then reduce the concentration of blood sugar. Therefore, it is clinically used as a drug for treating diabetes. [0003] In the process of microbial fermentation, carbon source is an important element that constitutes the composition of the bacteria, and it is also the main raw material for producing various metabolites and intracellular storage substances.Akobo sugar molecules have a pseudo -tetraogenic structure, which shows that its biological synthesis has a close relationship with the decomposition of carbon source substances.Studies o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/26C12R1/045
Inventor 陈学军曹云峰唐丽娜付海云陈培新李英然何茹贾伟娜马蕙陈乔杨子娇刘立峰谭锟
Owner HEBEI HUARONG PHARMA
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