Micro-fluidic chip for high-flux automatically rationing and mixing and use method and application of chip

Abstract

The invention discloses a micro-fluidic chip for high-flux automatically rationing and mixing and a use method and an application of the chip. The micro-fluidic chip is a combined micro-fluidic chip and consists of a micro-fluidic chip main body and a pre-degassing PDMS (polydimethyl siloxane) pump body which are combined; and based on the chip, negative pressure is produced in a closed micro-pipe system to form a fluid driving force by using the high dissolution property of the PDMS pump body to gases after de-gassing treatment, simultaneously a capillary valve is constructed by using the chip micro-pipe surface property and combining the geometric design, and automatic filling, rationing and mixing of fluid in the micro-fluidic chip main body are achieved through the coordinate action of the negative pressure driving and the capillary valve. Finally, the invention discloses the micro-fluidic chip used for high-flux screening of protein crystallization conditions.

Description

technical field [0001] The invention relates to a high-throughput automatic quantitative distribution and mixing microfluidic chip, a method of use and its application, which can be applied to the fields of chemistry, pharmacy and biology, especially for high-throughput screening of chemical synthesis and biochemical reaction conditions . Background technique [0002] Microfluidic chips have received more and more attention and popularity in recent years because of their advantages such as less sample consumption, fast analysis speed, good controllability, and easy integration. In particular, microfluidic chips integrating high-density and arrayed microfluidic units are easy to achieve parallelization and high-throughput reactions and screening, and have important application values ​​in the fields of chemistry and biology. In most cases, chemical and modern biological experiments are carried out in the state of aqueous solution, and often need to go through multiple steps ...

AI Technical Summary

Problems solved by technology

The mechanical pumps and valves used are generally divided into two types: integrated and separated. The integrated pumps and valves usually have complicated manufacturing process, high cost, and poor reliability in long-term work; while the separated pumps and valves are usually bulky Large, complex interface, high power consumption, poor portability

Therefore, the traditional microfluidic chip design and fluid manipulation methods severely limit the wide application of microfluidic technology in practice. , high-throughput, low-cost, automated detection and analysis platform needs

One of the inventors of this application has also designed and produced a self-quantitative distribution and mixing microfluidic chip based on capillary action, centrifugal force and capillary trap [G.Li, et al. "A compact disk-like centrifugal microfluidic system for high-throughput nanoliter-scale protein crystallization screening",Analytical Chemistry,2010,82(11):4362–4369.], but the fluid drive in this chip still requires external devices such as a relatively large centrifugal rotating platform, Greatly limit its portable applications (such as field environmental monitoring, bedside diagnosis, etc.)

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