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Marine surfactant-producing bacterial strain LHOD-1 and application thereof

A technology for producing surfactants and oceans, applied in the field of microorganisms, can solve problems such as the hazards of breeding resources, hypoxia, and death of marine organisms, and achieve the effect of improving degradation efficiency

Inactive Publication Date: 2013-03-27
LIAONING OCEAN & FISHERIES SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After the oil enters the seawater, a large amount of dissolved oxygen in the seawater is absorbed by the oil, and the oil film covers the water surface, which isolates the seawater from the atmosphere, causes seawater hypoxia, causes the death of marine organisms, and brings serious harm to the breeding resources.

Method used

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  • Marine surfactant-producing bacterial strain LHOD-1 and application thereof
  • Marine surfactant-producing bacterial strain LHOD-1 and application thereof
  • Marine surfactant-producing bacterial strain LHOD-1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1 The screening method of marine surfactant bacterial strain:

[0019] 1. Material preparation

[0020] 1. Bacterial source and experimental wastewater

[0021] (1) The bacterial source was the sediments at the scene of the 7.16 oil spill accident in Dalian City, Liaoning Province, which was polluted by petroleum hydrocarbons.

[0022] (2) Experimental waste water: 1000 mL of natural seawater, sterilized at 121 °C for 20 min, added 100 mg of diesel oil filtered through a 0.2 um microporous membrane.

[0023] 2. Medium

[0024] (1) Selection medium: 1000 mL of natural seawater, sterilized at 121 °C for 20 min, added 100 mg of diesel oil filtered through a 0.2 um microporous membrane.

[0025] (2) Separation medium: 5.0 g peptone, 1.0 g yeast extract, 0.1 g ferric phosphate, 1000 mL aged sea water, 20 g agar powder, pH 7.6-8.2, sterilized at 121 °C for 20 min.

[0026] (3) Activated expansion medium: peptone 5.0 g, yeast extract 1.0 g, ferric phosphate 0.1 ...

Embodiment 2

[0038] Example 2 Identification of marine surfactant-producing strain LHOD-1

[0039] 1. Identification of marine surfactant-producing strains

[0040] The physiological and biochemical identification and 16S rDNA molecular identification of the marine surfactant-producing halobacteria CGMCC 6715 were carried out, and the species of the strain was determined from the molecular level, combined with the analysis of the morphological characteristics and physiological and biochemical characteristics of the bacteria.

[0041] 16S rDNA sequence analysis mainly follows the following steps:

[0042] ① Extraction of bacterial nucleic acid DNA

[0043] Using TAKARA Bacterial Genomic DNA Extraction Kit to obtain the DNA of the strain

[0044] ②PCR amplification of 16S rDNA gene

[0045] P1: 27f (5'-AGA GTT TGA TCC TGG CTC AG-3')

[0046] P2: 1492r (5'-AAG TCG TAACAAGGT AAC C-3')

[0047] In a 50 uL system, add 1 uL template DNA (0.1 ug), 0.5 uL P1 and P2 (final concentration 0.5 uM)...

Embodiment 3

[0100] Example 3 Cell morphology, physiological and biochemical characteristics of marine surfactant-producing strain LHOD-1

[0101] See Table 2 for the physiological and biochemical characteristics of the marine surfactant-producing halobacteria CGMCC 6715, and see Table 2 for the cell morphology. figure 1 .

[0102] Table 2 Cell morphology, physiological and biochemical characteristics of the marine surfactant-producing strain LHOD-1

[0103]

[0104] Remarks: "+": more than 90% of the strains are positive; "-" more than 90% of the strains are negative.

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Abstract

The invention discloses a marine surfactant-producing bacterial strain. The bacterial strain belongs to Salinicolasp., and is preserved on October 29th,2012 with the preservation name of Salinicolasp.LHOD-1 and the preservation serial number of CGMCC No.6715 in China General Microbiological Culture Collection Center (CGMCC). The bacterial strain has the ability of producing surfactant, has stronger emulsion effect to petroleum hydrocarbon contamination in seas, can help to remove the petroleum hydrocarbon contamination in sea water, and has no positioning and pathogenic action to mariculture organisms.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a marine surfactant-producing bacterium living in salt fields (Salinicola sp.) and its application in treating seawater polluted by petroleum hydrocarbons. Background technique [0002] In recent years, the oil extraction industry has gradually moved from land to sea. The exploitation of oil and the development of the maritime transportation industry have resulted in frequent occurrence of offshore oil hydrocarbon pollution accidents caused by blowouts, oil spills, sinking of transport ships, and leakage of oil pipelines, and the scope of polluted sea areas is also expanding. After the oil enters the seawater, a large amount of dissolved oxygen in the seawater is absorbed by the oil, and the oil film covers the water surface, which isolates the seawater from the atmosphere, causes the seawater to lack oxygen, leads to the death of marine organisms, and brings serious...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/32
Inventor 关晓燕周遵春董颖王摆杨爱馥陈仲姜北蒋经伟高杉
Owner LIAONING OCEAN & FISHERIES SCI RES INST
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