Extractive containing total meroterpenoid of psidium guajave dialdehyde, and preparation method and application thereof

A technology of guava dialdehyde and extract, which is applied in the directions of medical preparations, pharmaceutical combinations, pharmaceutical formulations and the like containing active ingredients, can solve the problem of preparation methods and uses of guava dialdehyde total heterosource terpenoids that are not involved and other problems, to achieve the effects of easy large-scale production, simple preparation process, high yield and content

Active Publication Date: 2013-04-10
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent CN200610041855.4 introduces "preparation method of flavonoids from guava leaves", but this patent only involves the preparation method of total flavonoids from guava leaves, and the effect of total flavonoids on treating type Ⅱ diabetes and related complications
[0006] The above studies have shown that guava leaves have anti-tumor, anti-pathogenic microorganisms, and hypoglycemic pharmacological effects, but none of them involve the preparation method and use of guava dialdehyde total heterogeneous terpenoids in guava leaves. Not yet reported in the literature

Method used

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  • Extractive containing total meroterpenoid of psidium guajave dialdehyde, and preparation method and application thereof
  • Extractive containing total meroterpenoid of psidium guajave dialdehyde, and preparation method and application thereof
  • Extractive containing total meroterpenoid of psidium guajave dialdehyde, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take 500g of dried guava leaves after cutting, heat and reflux extraction with 5000ml of ethanol with a volume concentration of 50% for 2 times, each extraction for 1h, combine the two extracts, concentrate under reduced pressure, recover the ethanol until it has no alcohol smell, and the obtained concentrated The liquid is passed through a weakly polar macroporous adsorption resin column filled with AB-8, and impurities such as sugars are first eluted with water, and then other impurities are eluted with 5% ethanol; Deliquified, concentrated under reduced pressure at 65°C, dried under reduced pressure or freeze-dried, and measured by ultraviolet spectrophotometry to obtain an extract with a total heteroterpene content of guava dialdehyde of 32.3% (determination method is detailed in "Determination of total heteroterpenes in guava dialdehyde").

Embodiment 2

[0033] Take 500g of dried guava leaves after cutting, heat and reflux extraction with 5000ml of ethanol with a volume concentration of 60% for 2 times, each extraction for 1h, combine the two extracts, recover the ethanol under reduced pressure until it has no alcohol smell, and the obtained concentrated solution is passed through Filled with HPD450 medium-polar macroporous adsorption resin column, first elute sugar and other impurities with water, then elute other impurities with 10% ethanol; then elute with 90% ethanol, collect the ethanol eluate, and Concentrate under reduced pressure at 65°C, dry under reduced pressure or freeze-dry, and measure the content by ultraviolet spectrophotometry to obtain an extract with a total heteroterpene content of guava dialdehyde of 42%.

Embodiment 3

[0035]Take 500g of dried guava leaves after shredding, heat and reflux extraction with 5000ml of ethanol with a volume concentration of 70% for 2 times, each extraction for 1h, combine the two extracts, recover the ethanol under reduced pressure until it has no alcohol smell, and the obtained concentrated solution is passed through Filled with HPD100 non-polar macroporous adsorption resin column, first elute sugar and other impurities with water, then elute other impurities with 15% ethanol; then elute with 95% ethanol, collect the ethanol eluate, and Concentrate under reduced pressure at 65°C, dry under reduced pressure or freeze-dry, and measure the content by ultraviolet spectrophotometry to obtain an extract with a total heteroterpene content of 61% of guava dialdehyde.

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Abstract

The invention provides an extractive containing total meroterpenoid of psidium guajave dialdehyde, and a preparation method and application thereof. The preparation method comprises the following steps of: (1), removing impurities from psidium guajave leaves, shearing the psidium guajave leaves, and refluxing and extracting for 1-4 times by using ethanol; and mixing extracted solutions, filtering, decompressing and concentrating to obtain a concentrated solution of the psidium guajave leaves; (2), enabling the concentrated solution to flow through a macro-porous adsorption resin chromatographic column, and eluting by using water at first, then, eluting by using low-concentration ethanol with the volume concentration of 5-20%, and finally, eluting by using high-concentration ethanol with the volume concentration of 40-100%, and collecting a high-concentration ethanol eluant; and (3), decompressing, concentrating and drying the collected eluant so as to obtain the extractive containing the total meroterpenoid of psidium guajave dialdehyde. The method disclosed by the invention is simple in preparation process; the content of the extractive can be up to 30-80%; compared with extraction by directly using an organic solvent, the yield and the content are higher; the process is also more economic and environment-friendly; and the extractive disclosed by the invention can be used for preparing medicines and health-care products for preventing and curing type II diabetes, anti-tumor microorganisms and anti-viral microorganisms.

Description

technical field [0001] The invention relates to an extract containing guava dialdehyde total heteroterpene prepared from natural plant guava leaves, a preparation method and its application in medicine and health care products. Background technique [0002] Guava (Psidium guajava L), commonly known as plucked fruit, jaggery fruit, guava, red heart fruit, trumpet fruit, etc., is a plant of the genus Guava in the family Myrtaceae. Now widely distributed and planted in tropical and subtropical regions. [0003] Junyaprasert et al. found that guava leaf extract had a significant inhibitory effect on P-glycoprotein-mediated rhodamine 123 (Rho-123) efflux on Caco-2 cells (Junyaprasert VB, et a1.Inhibitory effect of Thai plant extracts on P- glycprotein mediated efflux[J]. Phytother Res, 2006, 20(1):79-8l); Manosroi et al studied the effect of 17 kinds of medicinal plants on the proliferation of epidermal cancer cells and murine leukemia cells, and found that the volatile oil comp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/61A61K31/352A61K31/11A61P3/10A61P35/00A61P31/00
Inventor 刘美凤谷灵灵周惠蒋利荣
Owner SOUTH CHINA UNIV OF TECH
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