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Core-shell type nano-contrast agent, preparation method and application thereof

A nano-contrast agent and reaction technology, applied in the field of core-shell nano-contrast agents, can solve the problems of reduced efficiency and sensitivity, and achieve the advantages of simple preparation method, low biological toxicity, and improved biocompatibility and tumor targeting. Effect

Active Publication Date: 2013-04-17
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In addition, nanoparticles with a diameter greater than 20 nm are easily phagocytosed by the reticuloendothelial system (RES), resulting in reduced efficiency and sensitivity.

Method used

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  • Core-shell type nano-contrast agent, preparation method and application thereof
  • Core-shell type nano-contrast agent, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] a, Preparation of gadolinium nanoclusters:

[0034] Dissolve 56mg of gadolinium chloride in 15mL of water to obtain gadolinium ion solution, add 15mL of gadolinium ion solution to 15mL of HSA solution (50mg / mL), mix well, then add 1.5mL of 1mol / L NaOH solution, adjust the pH value to strong base (pH10.0-14.0), the resulting mixture was placed on a shaking table at room temperature for 1 hour to form a gadolinium nanocluster solution (core-shell nanocontrast agent solution).

[0035] b. Purification of core-shell nano-contrast agents:

[0036] Transfer the obtained core-shell nano-contrast agent solution into a dialysis bag, place the dialysis bag in 500ml PBS buffer solution for dialysis for 24 hours at room temperature, change the new PBS buffer solution every 12 hours, and then place the dialysis bag in 500ml double distilled water Internal dialysis for 12 hours to remove small inorganic molecules in the solution.

[0037] c. Core-shell nano-contrast agent drying

...

Embodiment 2

[0041] a, Preparation of gadolinium nanoclusters:

[0042] Dissolve 112mg of gadolinium chloride in 20mL of water to obtain gadolinium ion solution, add 20mL of gadolinium ion solution to 30mL of HSA solution (50mg / mL), mix well, then add 2mL of 1mol / L NaOH solution, adjust the pH value to be strongly alkaline (pH10.0-14.0), the resulting mixture was placed on a shaking table at room temperature for 2 hours to form a gadolinium nanocluster solution (core-shell nanocontrast agent solution).

[0043] b. Purification of core-shell nano-contrast agents:

[0044] Transfer the obtained core-shell nano-contrast agent solution into a dialysis bag, place the dialysis bag in 500ml PBS buffer solution for 72 hours at room temperature, change the new PBS buffer solution every 12 hours, and then place the dialysis bag in 500ml double distilled water Internal dialysis for 6 hours to remove small inorganic molecules in the solution.

[0045] c. Core-shell nano-contrast agent drying

[004...

Embodiment 3

[0048] a, Preparation of gadolinium nanoclusters:

[0049] Dissolve 560mg of gadolinium chloride in 100mL of water to obtain gadolinium ion solution, add 100mL of gadolinium ion solution to 300mL of HSA solution (40mg / mL), mix well, then add 1mL of 2mol / L NaOH solution, adjust the pH value to be strongly alkaline (pH10.0-14.0), the resulting mixture was placed on a shaking table at room temperature for 5 hours to form a gadolinium nanocluster solution (core-shell nanocontrast agent solution).

[0050] b. Purification of core-shell nano-contrast agents:

[0051] Transfer the obtained core-shell nano-contrast agent solution into a dialysis bag, place the dialysis bag in 500ml PBS buffer solution for 72 hours at room temperature, change the new PBS buffer solution every 12 hours, and then place the dialysis bag in 500ml double distilled water Internal dialysis for 6 hours to remove small inorganic molecules in the solution.

[0052] c. Core-shell nano-contrast agent drying

[...

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Abstract

The invention discloses a core-shell type nano-contrast agent. The core-shell type nano-contrast agent comprises gadolinium ions and human serum albumin assembled outside the gadolinium ions and serving as a protecting group. The invention further provides a preparation method of the core-shell type nano-contrast agent. The core-shell type nano-contrast agent disclosed by the invention is combined with a nano-technology, and the human serum albumin is taken as a carrier for wrapping the paramagnetic metal gadolinium ions, thus MRI (magnetic resonance imaging) is realized, and biocompatibility and a tumor targeting property are improved; the prepared core-shell type nano-contrast agent has the advantages of small particle size and low biotoxicity; and the preparation method provided by the invention is simple.

Description

technical field [0001] The invention relates to molecular imaging technology, in particular to a core-shell nano contrast agent, its preparation method and application. Background technique [0002] Tumor is one of the important diseases that threaten human health. Early diagnosis and treatment of tumor is the key to improve the quality of life and cure rate of patients. At present, molecular imaging technologies applied to tumors include optical imaging technology, CT imaging technology, MRI molecular imaging technology, ultrasonic molecular imaging technology, SPECT technology, PET technology and near-infrared fluorescence imaging technology. Among them, MRI (Magnetic Resonance Imaging) is a medical imaging technology that uses different magnetic resonance signals generated by different tissues of the body under the influence of an external magnetic field for imaging. It has the advantages of realizing multi-nuclear and multi-parameter imaging, scanning in any orientation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/14
Inventor 蔡林涛胡德红盛宗海高笃阳张鹏飞龚萍
Owner SHENZHEN INST OF ADVANCED TECH
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