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Method for preparing protein nutrient solution by utilizing composite protease enzymolysis sesame dregs

A technology for compounding protease and sesame residue, applied in the fields of food and beverages, can solve the problems of high temperature, easy generation of toxic substances, etc., and achieve the effect of not easy to toxic substances and mild conditions.

Inactive Publication Date: 2013-05-01
HENAN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, hydrolysis of protein with hydrochloric acid requires a higher temperature (95°C - 110°C), and the hydrolysis process is prone to produce toxic substances-chloropropanol

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The present embodiment adopts the following steps to realize:

[0016] a, trypsin enzymolysis: take the dry sesame residue and pulverize it with a pulverizer, and sieve it with a 100-mesh sieve, add water to make a solution with a concentration of 3%, adjust the temperature to 45 ° C, and add trypsin according to the ratio of 0.0636 g / g, After enzymolysis for 2.5 hours, heat to 95°C for 10 minutes to inactivate the enzyme;

[0017] b. Enzymatic hydrolysis with papain: add papain at a ratio of 0.0073 g / g, enzymatic hydrolysis at a temperature of 60 °C for 2.5 hours, and heat inactivation. Centrifuge at 5000 r / min for 15 min to remove unhydrolyzed sesame residue protein and other insoluble substances, and take the supernatant;

[0018] c. Allocation and packaging: adding 3% sucrose, 3% β-cyclodextrin and 4‰ black candied jujube essence by weight to the supernatant in step b to prepare a clear and translucent, yellow-brown , No paste taste, sweet taste, suitable for drin...

Embodiment 2

[0020] According to the variety and origin of sesame raw material samples, the following steps can be adopted:

[0021] A. Trypsin enzymolysis: take the dried sesame residue and pulverize it with a pulverizer, sieve it with a 100-mesh sieve, add water to make a solution with a concentration of 3%, adjust the temperature to 45°C, and add trypsin at a ratio of 0.0636 g / g, After enzymatic hydrolysis for 3.5 hours, heat to 95°C for 10 minutes to inactivate the enzyme;

[0022] b Papain enzymatic hydrolysis: add papain at a ratio of 0.0073 g / g, enzymatic hydrolysis at a temperature of 60 °C for 2 hours, and heat inactivation. Centrifuge at 5000 r / min for 15 min to remove unhydrolyzed sesame residue protein and other insoluble substances, and take the supernatant;

[0023] c. Preparation and packaging: adding 4% sucrose, 3% β-cyclodextrin and 4‰ black candied jujube essence by weight to the supernatant in step b to prepare a clear and translucent, yellowish-brown, No paste taste, ...

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Abstract

The invention relates to a method for preparing protein nutrient solution by utilizing composite protease enzymolysis sesame dregs, and the method comprises the following steps of a, pulverizing dry sesame dregs through a pulverizing machine, screening pulverized sesame dregs through a 100-mesh sieve, charging water into the screened sesame dregs to prepare solution with the concentration of 2 to 5 percent, adjusting the temperature to 30 to 60 DEG C, adding trypsin according to the ratio of 0.03 to 0.08g / g, and conducting the enzyme deactivation by heating the mixture for 10 minutes after the enzymolysis for 2.5 hours; b, charging papain according to the ratio of 0.003 to 0.010g / g under the temperature condition of 30 to 80 DEG C for enzymolysis of 2.5 hours, and inactivating the mixture through heating; centrifuging the mixture for 15min at the rotation speed of 5000r / min, removing hydrolysis sesame dreg proteins and other insoluble matters, and extracting the supernate; and c, charging 2 to 8 percent by weight of cane sugar, 1 to 5 percent by weight of beta- cyclodextrin and 2 to 8 permillage by weight of flavoring black jujube essence into the supernate to prepare the sesame protein hydrolysis nutrients.

Description

technical field [0001] The invention relates to the technical fields of beverages and food, in particular to a method for preparing a protein nutrient solution by enzymatically hydrolyzing sesame residues with a composite protease. Background technique [0002] Sesame, also known as flax and linseed, is an annual herb of the Flax family and is one of the four major oil crops in my country. Sesame is rich in nutrition, and its oil content is generally between 46% and 62%, and its protein content is 20% to 22%. In the amino acid composition of sesame protein, except for the low lysine content, the rest are close to or reach the high-quality protein recommended by FAO / WHO. However, currently sesame residues are mainly used as fertilizer or animal feed, or even discarded as waste. The nutritional value and economic value of sesame protein have not been fully developed and utilized, resulting in waste of resources and even environmental pollution. [0003] At present, there ar...

Claims

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Application Information

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IPC IPC(8): A23L1/09A23L2/38A23L1/36A23L25/00
Inventor 侯利霞刘玉兰汪学德纪俊敏魏安池马宇翔张振山
Owner HENAN UNIVERSITY OF TECHNOLOGY
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