Pretreatment method capable of eliminating polyhydric alcohol matrix interference in tobacco essence analysis

Abstract

The invention discloses a pretreatment method capable of eliminating polyhydric alcohol matrix interference in tobacco essence analysis. The pretreatment method comprises the steps that 0.5g-1.5g of essence samples and 0.5g-6g of C18 silica gel packing are weighed and placed in a glass mortar, and stirred uniformly by a glass stirring rod; 10g of packing is weighed and subjected to column packing by using 30 mL of methyl alcohol by a wet method, and the process is repeated for three times; a 100 / 0, 70 / 30, 50 / 50 and 30 / 70 methyl alcohol / chloroform system is utilized to elute the samples, and eluents of three later gradients are combined; and an glass needle cylinder type injection syringe with an organic phase filtering membrane is used to absorb and combine the eluents for chromatographic analysis. According to the method, the problem of matrix interference caused by polyhydric alcohol such as ethylene glycol, 1, 4-butanediol, propylene glycol, glycerol and the like is effectively solved, and the effective components of the essence can be accurately, qualitatively and quantitatively analyzed; and moreover, in the pretreatment process, the method has the advantages that the test device is simple and convenient, the effective component loss of the essence is reduced, the step of decompressed concentration is omitted, the matrix interference can be remarkably eliminated, and the like.

Description

technical field [0001] The invention discloses a pretreatment method capable of eliminating the interference of polyalcohol matrix in the analysis of flavoring essence for tobacco, in particular a pretreatment method using polyalcohol as the matrix essence. Background technique [0002] Tobacco flavors often use compounds such as water, ethanol, ethylene glycol, 1,4-butanediol, propylene glycol, and glycerin as substrates. When performing GC / MS analysis (gas chromatography and mass spectrometry), ethylene glycol, The presence of polyhydric alcohols such as 1,4-butanediol, propylene glycol, and glycerin leads to obvious matrix interference problems, and significant tailing of chromatographic peaks, covering up many useful fragrance information and seriously affecting the analysis results. The existing pretreatment methods in the analysis of flavors by GC-MS mainly include solid-phase microextraction, purging and supplementary method, and stirring bar magnetic extraction-therm...

AI Technical Summary

Problems solved by technology

[0004] 1. There is a heating link in the extraction process, which is not suitable for the extraction of thermally unstable substances;

[0005] 2. It is not suitable for the extraction of highly polar and non-volatile substances such as polyols, and the tailing phenomenon is serious;

[0007] 1. It is not suitable for the extraction of highly polar and non-volatile substances such as polyols, and the tailing phenomenon is serious;

[0008] 2. The loss of active ingredients in the essence during the concentration process;

[0010] 1. The commercial stirring rod magnets have a single type, only PDMS (polydimethylsiloxane) non-polar magnets, and the extraction of highly polar and non-volatile substances such as polyols has serious tailing phenomenon;

[0011] 2. During thermal desorption of stirring bar magneton, the loss of polydimethylsiloxane material is serious, resulting in more column loss and magneton loss peaks in the chromatographic peaks;

[0012] 3. The instrument and its accessories are expensive

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