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A method for the analysis of volatile organic compounds in pleural effusion

A technology for volatile organic compounds and pleural effusion, which is applied in the direction of analyzing materials, separating materials, measuring devices, etc., to achieve the effect of eliminating matrix interference and easy operation

Active Publication Date: 2017-04-12
邳州市润宏实业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the tumor markers of pleural effusion in clinical application are mainly gene expression products and protein substances, but there are few reports on volatile organic compounds

Method used

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  • A method for the analysis of volatile organic compounds in pleural effusion
  • A method for the analysis of volatile organic compounds in pleural effusion
  • A method for the analysis of volatile organic compounds in pleural effusion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Analysis of pleural effusion samples by HS-SPME-cold trap focusing-GC / MS method

[0033] (1) Experimental method

[0034] Place the above-mentioned headspace bottle containing the pleural effusion sample to be tested on a magnetic stirrer, stir at a medium speed (800rmp) and heat to 50°C. After equilibrating for 3 minutes, extend the extraction head of the 75μm CAR / PDMS fiber out of the stainless steel protective needle tube , exposed to the space above the pleural effusion, after 20 minutes of extraction, retract the extraction head back into the needle tube, quickly insert the needle tube completely into the vaporization chamber of the gas chromatograph and push out the extraction head, and use the high temperature of the gasification chamber for analysis. During this period, the gas chromatographic column is immersed in a stainless steel cup filled with liquid nitrogen to form a cold trap focusing on a column range of 10 cm in length 20 cm from the column ...

Embodiment 2

[0048] Example 2: Detection and analysis of volatile organic compounds in different pleural effusion samples

[0049] 28 pleural effusion samples and clinical data were collected from 11 cancer patients (malignant group) and 17 benign disease patients (benign group), all of which were collected by thoracentesis from the Department of Respiratory Medicine, Zhejiang Provincial People's Hospital from January 2014 to 2014. Collected between December 2014. Once the cancer patients were confirmed and admitted to the hospital, the pleural effusion samples were taken on an empty stomach in the early morning of the next day, and the pleural effusion samples were also taken on an empty stomach in the early morning in the benign control group. The experimenters retrieved the samples with a sealed insulation box with built-in medical ice packs, and stored them in a -20°C refrigerator for later use. Of the 11 samples of malignant pleural effusion, 9 were lung cancer, 1 was colon cancer, a...

Embodiment 3

[0057] Example 3: Comparative analysis of volatile organic compounds in pleural effusion of patients with lung cancer and benign lung diseases

[0058] The pleural effusion samples and clinical data of 18 cases were collected from 9 lung cancer patients (cancer group) and 9 lung inflammation patients (inflammation group). Among the 9 lung cancer pleural effusion samples, there were 5 cases of adenocarcinoma and 4 cases of other The type of lung cancer was not specified. Among the 9 samples of benign pleural effusion, there were 7 cases of tuberculous pleurisy, 1 case of pneumonia, and 1 case of lung abscess.

[0059] (1) Analysis of the average peak area of ​​each organic compound

[0060] In the process of quantitative analysis and screening of differential substances, the occasional substances have no statistical significance and will cause certain errors in the results. Therefore, according to the qualitative results of 18 selected samples, 21 organic compounds with high d...

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Abstract

The invention provides an analysis method for a volatile organic compound in pleural effusion. The method comprises the steps of headspace solid phase microextraction, cold trap focusing and gas chromatographic and mass spectrometry combination. The method with the combination of the cold trap focusing and gas chromatographic and mass spectrometry combination on the basis of the headspace solid phase microextraction technology can rapidly and accurately analyze the volatile organic compound in the pleural effusion. According to the analysis method, extraction, enrichment and sample introduction are integrated into headspace solid phase microextraction, operation is easy and convenient, no complex pretreatment process is needed, complex matrix interference of biological samples is eliminated, and component information distortion in samples is not caused; due to the cold trap focusing technology, the high-volatility organic compound in the pleural effusion is condensed and secondarily analyzed at low temperature and effectively separated on the proper chromatographic condition. According to the analysis method, the volatile organic compound in the pleural effusion can be rapidly detected, quanlitative and semi-quantitative analysis is performed, and the analysis method is of great importance in distinguishing benign and malignant pleural effusion and even screening lung cancer markers by applying difference analysis.

Description

[0001] (1) Technical field [0002] The invention relates to a method for analyzing volatile organic compounds in pleural effusion, in particular to a method based on headspace solid-phase microextraction (HS-SPME) combined with cold trap focusing-gas chromatography-mass spectrometry (GC / MS). Accurate analysis of volatile organic compounds in pleural effusions. [0003] (2) Background technology [0004] Pleural effusion (Pleural Effusion) is the most specific human body fluid for lung diseases. It is the reflection of various diseases in the body on the pleura. It is mainly directly produced by lung lesions and is closely related to the development of diseases. There is also a small amount of fluid in the pleural cavity of normal people, which acts as a lubricant during respiratory movement. Generally, 500-1000mL of fluid is formed and absorbed every 24 hours in normal people. The fluid in the pleural cavity is reabsorbed from the venous end of the capillaries, and the rest o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 王丽丽张洁张佩佩刘会君黄忠平潘再法
Owner 邳州市润宏实业有限公司
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