Method and kit for detecting forbidden antibacterial drugs in cosmetics based on electrospray-ion mobility spectrometry and application

A technology of ion mobility spectrometry and antibacterial drugs, which is applied in the field of cosmetics detection, can solve the problems of unresolved cosmetic matrix specificity and reproducibility, no inspectors and experimental production areas, no mature antibacterial drugs, etc., to make up for sensitivity defects, Reduce the possibility of false negatives and make up for the effect of poor specificity

Pending Publication Date: 2022-05-06
江苏省食品药品监督检验研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microwave extraction has a good purification effect on cosmetic matrix, but it requires expensive instruments and digestive tubes, and the operation of the instrument consumes a lot of power, so it is not suitable for on-site inspections; techniques such as liquid phase microextraction and solid phase microextraction are easy to use, but the sample load is relatively large. Low, due to the use of less solvents, it is necessary to use different solvents to match the properties of cosmetics in order to achieve fully dispersed samples and accurate quantitative results, and the specificity is poor, often does not solve the specificity and

Method used

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  • Method and kit for detecting forbidden antibacterial drugs in cosmetics based on electrospray-ion mobility spectrometry and application
  • Method and kit for detecting forbidden antibacterial drugs in cosmetics based on electrospray-ion mobility spectrometry and application
  • Method and kit for detecting forbidden antibacterial drugs in cosmetics based on electrospray-ion mobility spectrometry and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] A method based on electrospray-ion mobility spectrometry detection of banned antibacterial drugs in cosmetics (acne-removing, children's skin care), the specific steps are as follows:

[0055] (1) Establish the ion mobility spectrum library of the antibacterial drugs to be detected in the samples to be tested

[0056] The 14 banned chemicals are as follows: Ofloxacin, Norfloxacin, Difloxacin, Sarafloxacin, Pefloxacin, Enoxacin (Enoxacin), Ciprofloxacin, Fluconazole, Naftifinehydrochloride, Ketoconazole, Bifonazole, Clotrimazole, Nitric Acid Econazole, Miconazole nitrate.

[0057] Standard solution configuration: Accurately weigh ofloxacin, norfloxacin, difloxacin, sarafloxacin, pefloxacin, enoxacin, ciprofloxacin, fluconazole, naphthalene hydrochloride Fen, ketoconazole, bifonazole, clotrimazole, econazole nitrate, and miconazole nitrate, 5.0 mg each of 14 compounds were placed in 25 mL measuring bottles, dissolved with 80% methanol and diluted to the mark. Prepared ...

Embodiment 2

[0069] A test kit for the detection method described in Example 1, such as figure 2As shown, including A: 10mL polyplastic centrifuge tube; B: 80% acetonitrile solution (containing 0.2% trichloroacetic acid); C: after pretreatment (washing with 3mL of methanol and 3mL of 1% trichloroacetic acid solution in sequence, then air-dried ) MCX solid-phase extraction column (3mL / 60 mg); D: methanol; E1: 2mL glass vial; E2: 2% ammonia solution in methanol (V / V); E3: 2mL syringe plunger.

Embodiment 3

[0071] The method for prohibiting antimicrobials in the detection of cosmetics based on the test kit described in Example 2 (Biderui Hyaluronic Acid Moisturizing and Repairing Beautifying Milk), the specific steps are as follows:

[0072] (1) Establish the ion mobility spectrum library of the antibacterial drug to be detected in the sample to be tested, the method is the same as that of step (1) in Example 1.

[0073] (2) Sample pretreatment: such as image 3 As shown, take about 0.5mL of the sample to be tested, put it in a 10mL centrifuge tube (A), add about 5mL of solvent B, vortex for 30s, 5000r min -1 Centrifuge for 5min; put the supernatant on the solid-phase extraction column C; wait for the solution to flow out naturally (or put it on the solid-phase extraction apparatus to drain out under reduced pressure), discard the solution that flows out; add eluent (solution D) for about Put 3mL of eluent (solution E2) on the solid-phase extraction column C, discard the eluent ...

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Abstract

The invention discloses a method and a kit for detecting forbidden antibacterial drugs in cosmetics based on electrospray-ion mobility spectrometry and application. The method comprises the following steps: firstly, establishing an ion mobility spectrometry library of antibacterial drugs to be detected in a sample to be detected; then extracting a sample, and purifying and concentrating the sample through cation solid-phase extraction; then determining components of the sample by adopting electrospray-ion mobility spectrometry; and finally, judging whether the sample contains the forbidden antibacterial drug or not by using an ion mobility spectrometry library or comparing migration time. The matched kit comprises reagents and experimental apparatuses required by sample pretreatment. According to the method disclosed by the invention, an optimized sample pretreatment method is adopted, so that matrix interference in cosmetics can be effectively eliminated, and occurrence of false positive is reduced; the inhibition effect of the surfactant in the cosmetics is obviously reduced, and the extraction solution is concentrated, so that the occurrence of false negative is reduced. The kit is simple to operate, easy to master and carry, does not need professionals and precise pretreatment instruments, and is suitable for on-site screening.

Description

technical field [0001] The invention belongs to the technical field of cosmetic detection, and in particular relates to a method, kit and application for detecting prohibited antibacterial drugs in cosmetics based on electrospray-ion mobility spectrometry. Background technique [0002] With the gradual development of the national economy, people's pursuit of beauty is getting higher and higher, and the demand for cosmetics is also increasing. In order to achieve good results and quick results, illegal businesses illegally add various prohibited chemicals to cosmetics, causing consumers There are some adverse reactions after use. Among them, antibacterial drugs mainly include antifungal drugs and synthetic antibiotics, which belong to prescription drugs and are randomly added to cosmetics, which can easily cause contact dermatitis, manifested as erythema, edema, erosion, desquamation, exudation, itching, burning and other symptoms, and even cause serious health problems. Ac...

Claims

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Application Information

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IPC IPC(8): G01N27/622
CPCG01N27/622Y02A50/30
Inventor 孙晶曹玲冯有龙方方
Owner 江苏省食品药品监督检验研究院
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