Method for detecting chlorophyllin copper in olive oil

A technology of copper chlorophyll and a detection method is applied in the directions of measuring device, measurement of color/spectral characteristics, material analysis by optical means, etc., can solve problems such as copper chlorophyll in olive oil, which is not seen, and achieves easy popularization, rapid detection, and high efficiency. low cost effect

Active Publication Date: 2017-11-24
青岛市食品药品检验研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no detection method for copper chlorophyllin in olive oil

Method used

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  • Method for detecting chlorophyllin copper in olive oil
  • Method for detecting chlorophyllin copper in olive oil
  • Method for detecting chlorophyllin copper in olive oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Use 671nm wavelength to calibrate the absorbance, the method of correcting absorbance is 653nm absorbance / 671nm absorbance, the calibration curve y=0.0737x+0.3441, R²=0.9935, the recovery rate of the same olive oil is 85%-103%, the standard deviation of the blank value S= 0.0694, the limit of detection LOD=3S / K=2.83mg / Kg, K=0.0734 is the slope of the linear equation. See Table 1-3 for detailed data.

[0059] Table 1 embodiment one standard curve data

[0060] name

Abs

Abs

Concentration (mg / kg)

Abs / Abs

st0-1

0.13582

0.39922

0

0.3402

st0-2

0.13482

0.39822

0

0.3386

st1-1

0.15311

0.3981

0.7142

0.3846

st1-2

0.15656

0.40219

0.7142

0.3893

st2-1

0.2014

0.41126

1.9911

0.4897

st2-2

0.20419

0.41464

1.9911

0.4925

st3-1

0.25263

0.4218

3.3053

0.5989

st3-2

0.25802

0.42765

3.3053

0.6033

st4-1

0.29759 ...

Embodiment 2

[0066] Use 671nm wavelength to calibrate the absorbance, the wavelength calibrated absorbance method is 653nm absorbance-0.3*671nm absorbance, the calibration curve y=0.034x+0.0139, R²=0.9973, the recovery rate of the same olive oil is 88%-102%, the standard of blank value Deviation S=0.0206, detection limit LOD=3S / K=1.82mg / Kg, K=0.034 is the slope of the linear equation.

[0067] Table 4 embodiment two standard curve data

[0068] name

Abs

Abs

Concentration (mg / kg)

wavelength correction value

st0-1

0.13582

0.39922

0

0.0161

st0-2

0.13482

0.39822

0

0.0154

st1-1

0.15311

0.3981

0.7142

0.0337

st1-2

0.15656

0.40219

0.7142

0.0359

st2-1

0.2014

0.41126

1.9911

0.0780

st2-2

0.20419

0.41464

1.9911

0.0798

st3-1

0.25263

0.4218

3.3053

0.1261

st3-2

0.25802

0.42765

3.3053

0.1297

st4-1

0.29759

0.43772

4....

Embodiment 3

[0074] Use 671nm and 800nm ​​wavelengths to calibrate the absorbance, the corrected absorbance method is 653nm absorbance-0.3*671nm absorbance-0.7*800 absorbance, the calibration curve y=0.0337x-0.0032, R²=0.9968, the recovery rate of the same olive oil addition is 85%-97 %, the standard deviation of the blank value S=0.0241, the detection limit LOD=3S / K=2.15mg / Kg, K=0.0337 is the slope of the linear equation.

[0075] Table 7 Example three standard curve data

[0076]

[0077] Table 8 Example 3 Addition recovery experimental data

[0078]

[0079] Table 9 Example three blank value standard deviation experimental data

[0080]

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Abstract

The invention discloses a method for detecting chlorophyllin copper in olive oil. The method is characterized by comprising the following steps: I, preparing a chlorophyllin copper standard stock solution from grade-I soybean oil as a diluents without characteristic absorption wavelength at 360-860 nm; II, preparing a chlorophyllin copper standard use solution from blank olive oil without chlorophyllin copper; III, detecting a sample by using a spectrophotometer, and acquiring an average value of one or more than two wavelength absorbancy degrees of 400-440 nm or 620-660 nm as characteristic absorbancy of the chlorophyllin copper in a sample; IV, correcting the absorbancy by using one or more wavelengths of 360-400 nm, 440-540 nm and 660-800 nm; V, quantifying by using an external standard method, thereby obtaining the content of the chlorophyllin copper. The method has the advantages of being visible, rapid, convenient, developed, cheap and the like.

Description

technical field [0001] The invention relates to the technical field of detecting illegal additives in olive oil, in particular to a method for detecting copper chlorophyllin in olive oil. Background technique [0002] The derivatives of chlorophyll, copper chlorophyll, sodium copper chlorophyllin and potassium copper chlorophyllin, are widely used in the food industry as coloring agents. Copper chlorophyll (copperchlorophyll), CNS No. 08.153, INS No. 141i, is a fat-soluble compound. my country's national standard GB2760-2014 "National Food Safety Standard Food Additive Use Standard" stipulates that copper chlorophyll sodium can be used as a coloring agent in cream, candy , baked goods. Chlorophyllin copper sodium salt, chlorophyll copper potassium salt (chlorophyllin copper complex, sodium and potassium salts) CNS No. 08.009, INS No. 141ii, is a water-soluble compound, my country’s national standard GB2760-2014 "National Food Safety Standard Food Additive Use Standards" stip...

Claims

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Application Information

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IPC IPC(8): G01N21/31
CPCG01N21/314G01N2021/3148
Inventor 蒋万枫张宁张凤艳
Owner 青岛市食品药品检验研究院
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