Method for extracting natural saponin and biosurfactant composite product

A bio-surface and active agent technology, applied in the field of extracting natural and bio-surfactant composite products, can solve the problems of insufficient and efficient utilization of carbohydrates and high surface tension of natural saponin, reducing energy consumption and efficient utilization of raw materials , the effect of mild reaction conditions

Active Publication Date: 2013-05-08
广州德谷个人护理用品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Although the above technical scheme has the advantages of simplified process and high production efficiency, the carbohydrates in the aqueous sap

Method used

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  • Method for extracting natural saponin and biosurfactant composite product
  • Method for extracting natural saponin and biosurfactant composite product
  • Method for extracting natural saponin and biosurfactant composite product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Such as figure 1 Shown, the method concrete process of extracting natural saponin and biosurfactant composite product of the present invention is as follows:

[0073] The air-dried Sapindus fruit peel is removed to remove impurities and iron, the electric pulverizer pulverizes the peel, passes through a 12-mesh sieve, and vibrates and extracts saponin in a constant temperature shaker at 60°C and 150 revolutions, wherein the weight ratio of raw materials to water is 1: 6. The extraction time is 5h. Place the extract in a centrifuge with a rotating speed of 3000rpm, centrifuge for 15min, and collect the supernatant, which is the aqueous extract of Sapindus chinensis.

[0074] With 10% NaOH solution, add 1-2 drops dropwise to adjust the pH value of the solution to 4.8. Add 450 IU cellobiase / L to the solution, and carry out saccharification and hydrolysis for 96 hours in a full-temperature shaking incubator at 45° C. and 180 rpm.

[0075] The enzyme hydrolyzate was used ...

Embodiment 2

[0077] Such as figure 1 Shown, the method concrete process of extracting natural saponin and biosurfactant composite product of the present invention is as follows:

[0078] Remove impurities and iron from the air-dried acacia pods, crush the pericarp with an electric pulverizer, pass through a 12-mesh sieve, and extract saponin by vibrating in a constant temperature shaker at 60°C and 150 rpm, wherein the weight ratio of raw materials to water is 1:8, The extraction time is 5h. The extract is placed in a centrifuge with a rotating speed of 3000rpm, centrifuged for 15min, and the supernatant is collected, which is the aqueous extract of saponins.

[0079] With 10% NaOH solution, add 1-2 drops dropwise to adjust the pH value of the solution to 4.8. Add 450 IU cellobiase / L to the solution, and carry out saccharification and hydrolysis for 96 hours in a full-temperature shaking incubator at 45° C. and 180 rpm.

[0080] The enzyme hydrolyzate was used as the fermentation substr...

Embodiment 3

[0082] Such as figure 1 Shown, the method concrete process of extracting natural saponin and biosurfactant composite product of the present invention is as follows:

[0083] The air-dried Sapindus fruit peel is removed to remove impurities and iron, the electric pulverizer pulverizes the peel, passes through a 20-mesh sieve, and vibrates and extracts saponin in a constant temperature shaker at 60°C and 150 revolutions, wherein the weight ratio of raw materials to water is 1: 2. The extraction time is 4h. Place the extract in a centrifuge with a rotating speed of 3000rpm, centrifuge for 15min, and collect the supernatant, which is the aqueous extract of Sapindus chinensis.

[0084] With 10% NaOH solution, add 1-2 drops dropwise to adjust the pH value of the solution to 4.8. Add 450 IU cellobiase / L to the solution, and carry out saccharification and hydrolysis for 96 hours in a full-temperature shaking incubator at 45° C. and 180 rpm.

[0085] The enzyme hydrolyzate was used ...

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Abstract

The invention provides a method for extracting a natural saponin and biosurfactant composite product. According to the fact that soapberry or Chinese honeylocust water extract contains cellobiose and monosaccharide in high concentration, the cellobiose is subjected to enzyme hydrolysis, water extraction enzyme hydrolysate is taken as a substrate, hilly candida and pseudomonas aeruginosa are inoculated, sophorolipid and rhamnolipid are produced by fermentation, the natural surfactant and biosurfactant composite product is obtained, and a practicable and efficient technical route is provided for preparing a natural saponin liquid detergent or other liquid products.

Description

technical field [0001] The invention relates to a method for extracting composite products of natural and biological surfactants, which belongs to the field of biomass chemistry and chemical industry. Background technique [0002] Natural saponin, also known as saponin, is a general term for plant glycosides that can form aqueous or colloidal solutions and form soapy foam. It is composed of saponin and sugar, uronic acid or other organic acids, which can effectively reduce the surface tension of water. It is a natural, non-toxic and non-polluting surfactant. [0003] Sapindus mukurossi Gaertn., also known as Sapindus mukurossi Gaertn., is a large deciduous tree with a height of more than 20 meters. The skin is taupe or dark brown, mainly distributed in the east, south and southwest of my country. The root and fruit are used as medicine, which has the functions of clearing heat and detoxifying, reducing phlegm and relieving cough. Sapindus husk contains about 15 to 25% Sapi...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P19/12C12P19/02B01F17/56C12R1/72C12R1/385C09K23/56
Inventor 蒋建新赵丹青周自圆孙达峰朱莉伟翁震
Owner 广州德谷个人护理用品有限公司
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