Method for extracting natural saponin and biosurfactant composite product
A bio-surface and active agent technology, applied in the field of extracting natural and bio-surfactant composite products, can solve the problems of insufficient and efficient utilization of carbohydrates and high surface tension of natural saponin, reducing energy consumption and efficient utilization of raw materials , the effect of mild reaction conditions
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Embodiment 1
[0072] Such as figure 1 Shown, the method concrete process of extracting natural saponin and biosurfactant composite product of the present invention is as follows:
[0073] The air-dried Sapindus fruit peel is removed to remove impurities and iron, the electric pulverizer pulverizes the peel, passes through a 12-mesh sieve, and vibrates and extracts saponin in a constant temperature shaker at 60°C and 150 revolutions, wherein the weight ratio of raw materials to water is 1: 6. The extraction time is 5h. Place the extract in a centrifuge with a rotating speed of 3000rpm, centrifuge for 15min, and collect the supernatant, which is the aqueous extract of Sapindus chinensis.
[0074] With 10% NaOH solution, add 1-2 drops dropwise to adjust the pH value of the solution to 4.8. Add 450 IU cellobiase / L to the solution, and carry out saccharification and hydrolysis for 96 hours in a full-temperature shaking incubator at 45° C. and 180 rpm.
[0075] The enzyme hydrolyzate was used ...
Embodiment 2
[0077] Such as figure 1 Shown, the method concrete process of extracting natural saponin and biosurfactant composite product of the present invention is as follows:
[0078] Remove impurities and iron from the air-dried acacia pods, crush the pericarp with an electric pulverizer, pass through a 12-mesh sieve, and extract saponin by vibrating in a constant temperature shaker at 60°C and 150 rpm, wherein the weight ratio of raw materials to water is 1:8, The extraction time is 5h. The extract is placed in a centrifuge with a rotating speed of 3000rpm, centrifuged for 15min, and the supernatant is collected, which is the aqueous extract of saponins.
[0079] With 10% NaOH solution, add 1-2 drops dropwise to adjust the pH value of the solution to 4.8. Add 450 IU cellobiase / L to the solution, and carry out saccharification and hydrolysis for 96 hours in a full-temperature shaking incubator at 45° C. and 180 rpm.
[0080] The enzyme hydrolyzate was used as the fermentation substr...
Embodiment 3
[0082] Such as figure 1 Shown, the method concrete process of extracting natural saponin and biosurfactant composite product of the present invention is as follows:
[0083] The air-dried Sapindus fruit peel is removed to remove impurities and iron, the electric pulverizer pulverizes the peel, passes through a 20-mesh sieve, and vibrates and extracts saponin in a constant temperature shaker at 60°C and 150 revolutions, wherein the weight ratio of raw materials to water is 1: 2. The extraction time is 4h. Place the extract in a centrifuge with a rotating speed of 3000rpm, centrifuge for 15min, and collect the supernatant, which is the aqueous extract of Sapindus chinensis.
[0084] With 10% NaOH solution, add 1-2 drops dropwise to adjust the pH value of the solution to 4.8. Add 450 IU cellobiase / L to the solution, and carry out saccharification and hydrolysis for 96 hours in a full-temperature shaking incubator at 45° C. and 180 rpm.
[0085] The enzyme hydrolyzate was used ...
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