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Identification method of oligofructose inulin purity

A technology of fructooligosaccharides and identification methods, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to correctly detect the purity and complexity of products

Active Publication Date: 2013-05-08
JIANGSU ALAND NOURISHMENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using HPLC method, the method of measuring inulin content with a certain monomer component of inulin polysaccharide as a standard is relatively complicated, because the monomer component of inulin in China is not easy to obtain, so the current routine analysis method sulfuric acid-phenol method cannot be detected correctly The purity of the product

Method used

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  • Identification method of oligofructose inulin purity
  • Identification method of oligofructose inulin purity
  • Identification method of oligofructose inulin purity

Examples

Experimental program
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Effect test

Embodiment 1

[0023] 1) Preparation of fructose reference substance solution: accurately weigh 50 mg of fructose reference substance into a 5ml volumetric flask, and prepare fructose standard solution with a concentration of 10mg / ml, and pass through 0.45um to be tested; preparation of glucose reference substance solution: accurately weigh glucose reference substance 10mg to 10ml volumetric flask, prepare glucose standard solution with a concentration of 1mg / ml, pass through 0.45um to be tested;

[0024] 2) Setting of chromatographic conditions: detector: differential refractive index detector, detector temperature 50°C; chromatographic column: Phenomenex Rezex Ca 2+ ;Column temperature: 80°C; Mobile phase: purified water; Flow rate: 0.50ml / min; Injection volume: 25μ1; Running time: 30min;

[0025] 3) Inulin sample treatment: Test product (1): Precisely weigh 1.0 g of inulin sample, put it in a 100ml volumetric flask, add appropriate amount of water, heat to dissolve, cool to the greenhous...

Embodiment 2

[0029] 1) Preparation of fructose reference substance solution: accurately weigh 50 mg of fructose reference substance into a 5ml volumetric flask, and prepare fructose standard solution with a concentration of 10mg / ml, and pass through 0.45um to be tested; preparation of glucose reference substance solution: accurately weigh glucose reference substance 10mg to 10ml volumetric flask, prepare glucose standard solution with a concentration of 1mg / ml, pass through 0.45um to be tested;

[0030] 2) Setting of chromatographic conditions: detector: differential refractive index detector, detector temperature 50°C; chromatographic column: Phenomenex Rezex Ca 2+ ;Column temperature: 80°C; Mobile phase: purified water; Flow rate: 0.50ml / min; Injection volume: 25μ1; Running time: 35min;

[0031] 3) Inulin sample treatment: Test product (1): Precisely weigh 1.0 g of inulin sample, put it in a 100ml volumetric flask, add appropriate amount of water, heat to dissolve, cool to the greenhous...

Embodiment 3

[0035]1) Preparation of fructose reference substance solution: accurately weigh 50 mg of fructose reference substance into a 5ml volumetric flask, and prepare fructose standard solution with a concentration of 10mg / ml, and pass through 0.45um to be tested; preparation of glucose reference substance solution: accurately weigh glucose reference substance 10mg to 10ml volumetric flask, prepare glucose standard solution with a concentration of 1mg / ml, pass through 0.45um to be tested;

[0036] 2) Setting of chromatographic conditions: detector: differential refractive index detector, detector temperature 50°C; chromatographic column: Phenomenex Rezex Ca 2+ ;Column temperature: 80°C; Mobile phase: purified water; Flow rate: 0.50ml / min; Injection volume: 25μ1; Running time: 35min; Running time: 40min;

[0037] 3) Inulin sample treatment: Test product (1): Precisely weigh 1.0 g of inulin sample, put it in a 100ml volumetric flask, add appropriate amount of water, heat to dissolve, c...

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Abstract

The invention discloses an identification method of an oligofructose inulin purity. The method comprises the following steps of: carrying out enzymatic hydrolysis on inulin by using inulase, then determining contents of fructose and glucose in a sample before the enzymatic hydrolysis and after the enzymatic hydrolysis of oligofructose inulin by using HPLC(High Performance Liquid Chromatography), respectively calculating content differences of fructose and glucose before the enzymatic hydrolysis and after the enzymatic hydrolysis, and multiplying the sum of the percentage difference before the enzymatic hydrolysis and after the enzymatic hydrolysis of fructose and the percentage difference before the enzymatic hydrolysis and after the enzymatic hydrolysis of glucose by 0.9 to be converted into the purify of inulin. The identification method particularly comprises the following steps of: 1) preparing a fructose and glucose reference solution; 2) setting chromatographic conditions; 3) treating an inulin sample; 4) analyzing and calculating the contents of fructose and glucose by using the HPLC method; and 5) identifying the inulin purity. Through the identification method of the oligofructose inulin purity disclosed by the invention, enzymatic hydrolysis is carried out on inulin by using inulase, then the HPLC is used to analyze changes of fructose and glucose before the enzymatic hydrolysis and after the enzymatic hydrolysis of oligofructose inulin, and finally the inulin purity is accurately identified, thereby judging whether a product is qualified or not. In comparison with the traditional method, the identification method is more scientific and reasonable.

Description

technical field [0001] The invention relates to an HPLC method for determining the difference in fructose and glucose contents between inulin before enzymolysis and inulin after enzymolysis, and calculating the true purity of the inulin. Background technique [0002] Inulin (Inulin) belongs to the linear inulin-type fructan, as a natural functional polysaccharide, it has the physiological function of water-soluble dietary fiber and biological activity prerequisites, and has a super strong function of proliferating bifidobacteria, which is very important for regulating Balance the body, restore gastrointestinal function, improve immunity, promote the absorption of mineral calcium, magnesium, zinc, copper, etc., regulate blood sugar, lower blood lipids, prevent obesity and anti-cancer and other physiological functions, prevent various diseases, and maintain good health It plays an extremely important role and can be widely used in low-calorie, low-sugar, and low-fat foods. Ac...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 高平陈华李荣张国栋张沛霞
Owner JIANGSU ALAND NOURISHMENT
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