Method for analyzing generating trend of aflatoxin B1 in peanut meal by multiple PCR (Polymerase Chain Reaction) technology

Abstract

The invention discloses a method for analyzing generating trend of aflatoxin B1 in peanut meal by a multiple PCR (Polymerase Chain Reaction) technology. The method belongs to the field of microbial and molecular biology and comprises the following steps of: extracting the genome DNA (Deoxyribonucleic Acid) of peanut meal through a soil DNA extraction kit; synthesizing a metabolic pathway through aflatoxin B1 to design a key enzyme gene design primer sequence; performing PCR identification for potential producing strain of aflatoxin; and guiding storage and conveyance condition control through the PCR result. The method further provides a scientific ground for dynamic change and control of aflatoxin B1 in peanut meal and has the advantages of fast speed and high reliability.

Description

technical field [0001] The invention belongs to the application field of microorganisms and molecular biology, in particular to a method for analyzing aflatoxin B in peanut meal by using multiple PCR technology 1 method of generating trends. Background technique [0002] Peanut meal is highly susceptible to microorganisms such as Aspergillus flavus and Aspergillus parasiticus, and produces aflatoxin under certain temperature and humidity conditions. Aflatoxins are the most toxic type of biological toxins found in contaminated agricultural products, and have strong carcinogenic, mutagenic and teratogenic properties; especially aflatoxin B 1 , the toxicity is 10 times and 68 times that of potassium cyanide and arsenic, respectively; the pollution of aflatoxin has become the bottleneck of large-scale application of many agricultural products and a potential threat to the safety of human and animal life. [0003] Aflatoxin B 1 The identification of the producing bacteria is v...

AI Technical Summary

Problems solved by technology

Aflatoxins are the most toxic type of biological toxins found in contaminated agricultural products, and have strong carcinogenic, mutagenic and teratogenic properties; especially aflatoxin B 1 , the toxicity is 10 times and 68 times that of potassium cyanide and arsenic respectively; the pollution of aflatoxin has become the bottleneck of large-scale application of many agricultural products and a potential threat to the safety of human and animal life

[0003] Aflatoxin B 1 The identification of the producing bacteria is very difficult. Traditional morphological methods are mainly used to observe the morphological characteristics of the microorganisms through an optical microscope and the morphological analysis of a single colony on a plate. 1 Producers are distinguished from other non-aflatoxin-producing molds. Although there are many traditional methods and molecular biology methods for the detection of A. Large interference, difficult to achieve

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