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Isaria fumosoroseus and application thereof for preventing and treating bemisia tabaci

A technology of Corynesporium fumigatus and Bemisia tabaci, which is applied to high-efficiency Corynesporium species and its application in the control of Bemisia tabaci, and can solve the problems of large-scale application of fungal insecticides with unstable control effects, etc. To achieve the effect of non-toxicity for humans and animals

Active Publication Date: 2014-09-24
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the control effect of existing entomopathogenic fungi to control whitefly is unstable and other reasons limit the large-scale application of fungal insecticides, the technical field needs new stable and efficient entomopathogenic fungus technology

Method used

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  • Isaria fumosoroseus and application thereof for preventing and treating bemisia tabaci
  • Isaria fumosoroseus and application thereof for preventing and treating bemisia tabaci
  • Isaria fumosoroseus and application thereof for preventing and treating bemisia tabaci

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] In August 2012, in Guangdong, China, the inventors collected naturally susceptible whitefly worms, isolated and purified the strains, measured the pathogenicity, carefully compared the biological characteristics and production traits, identified the strains, and screened repeatedly , obtained a strain highly effective to whitefly, and the taxonomic status of said strain belongs to Eumycota, Mitosporic fungi, Hyphomycetes, Hyphomycetales, Hyphomycetes family (Hyphomycetaceae), Corynespora ( Isaria ). No sexual generation of this strain was found.

[0023] The strain ( Isaria fumosoroseus ) IfB01 has been deposited in China Center for Type Culture Collection (CCTCC), the date of deposit is October 12, 2012, and the deposit number is CCTCC M 2012400.

[0024] The morphological characteristics of the described Corynesporium fumigatus strain: On Cha's culture medium, initially the colony is regular and round, and the hyphae are white; after prolonged cultivation, the co...

Embodiment 2

[0026] Example 2 Production of conidia

[0027] Preparation of spore suspension: pick conidia from the slant bacteria, insert them into 0.05% Tween-80 solution that has been sterilized, measure the spore concentration with a hemocytometer, and adjust to 10 7 conidia / mL. spare.

[0028] Seed culture: Inoculate 2 mL of spore suspension into 98 mL of seed medium, and shake the flask at 180 r / m and 26°C for 2 to 3 days to form a seed liquid. The seed medium is Czapek-Dox medium with 0.5% peptone, and its components are: protein Chen 5 g, NaN0 3 3 g, K 2 HP0 41 g, MgS0 4 · 7H 2 0 0.5 g, KCl 0.5 g, FeSO 4 · 7H 2 0 0.01 g, molasses 30 g, agar 15-20 g, water 1000 mL.

[0029] Solid fermentation: Steam the rice first, put it into a fresh-keeping bag, wait for it to cool to room temperature, inoculate the above seed liquid, the inoculation ratio is 5-15% (V / W, mL / g), and inoculate at 25-28°C Cultivate for about 2 weeks, and when the surface of the rice grains is covered w...

Embodiment 3

[0030] Example 3 Control of Bemisia tabaci by Corynesporium fumigatus

[0031] Apply the conidia produced in the above-mentioned embodiment 2, under laboratory conditions, the spore suspension is inoculated on the B-type Bemisia tabaci nymphs on potted broccoli seedlings, and the incidence of Bemisia tabaci is investigated at different times after treatment , the results showed that the effect of this strain on whitefly nymphs was very good, and the semi-lethal concentration to 2nd instar whitefly nymphs was 7.5×10 4 days after inoculation. 5 conidia / mL, 7.8×10 for 3rd instar Bemisia tabaci nymphs 5 conidia / mL, as shown in Table 1.

[0032] Apply the conidia produced in the above-mentioned embodiment 2 to prevent and control field Bemisia tabaci, and at the peak period of Bemisia tabaci nymphs, 1 × 10 7 30 L per mu of conidia / mL conidia suspension was sprayed on the type B whitefly on tomato grown in the open field, and the population decline rate was investigated at diffe...

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Abstract

The invention discloses a strain of Corynesium fumosoroseus (Isariafumosoroseus) and its application in preventing and treating Bemisia tabaci. The strain is preserved in the China Type Culture Collection Center, the preservation date is October 12, 2012, the preservation number CCTCC NO: M2012400, and the taxonomic status belongs to Eumycota, Mitosporic fungi, Hyphomycetes ), Hyphomycetales, Hyphomycetaceae, Isaria. Corynesporium fumigata rosea strain of the present invention is highly pathogenic to type B whitefly, and its semi-lethal concentration (LC50) to 2nd instar nymphs is 7.5 × 105 conidia / mL (the 4th day after inoculation) , the field control effect on Bemisia tabaci can reach more than 90%, which is equivalent to the chemical insecticide spirotetramat. As a live biopesticide, the bacterial strain has no pollution to the environment and no toxicity to humans and animals, and is suitable for the production of organic agricultural products.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and in particular relates to a high-efficiency Corynesporium sp. and its application in preventing and treating Bemisia tabaci. Background technique [0002] whitefly Bemisia tabaci (Gennadius) is a worldwide important agricultural and forestry pest, which causes serious economic losses. There are dozens of biotypes of Bemisia tabaci, among which type B and type Q are the most dangerous pests. Type B bemisia invaded my country in the 1990s, and then Q type bemisia also invaded our country. These two biotypes have widely harmed the main agricultural and forestry crops in most parts of our country, and have caused tens of billions of dollars. Economic losses. Bemisia tabaci has a wide range of hosts, many biotypes, and many generations per year. It is easy to form drug resistance, so it is extremely difficult to control. [0003] Bemisia tabaci is a polyphagous pest with a wide r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
Inventor 胡琼波邹春华
Owner SOUTH CHINA AGRI UNIV
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