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Cutting propagation method of strengthening antipollution factor zelkova schneideriana

A cutting propagation and anti-pollution technology, applied in the field of plant propagation, can solve the problems of vitrification, low rate of adventitious bud induction, beech trees are limited to the laboratory stage, etc., achieve good dispersibility and weather resistance, and strong ultraviolet shielding effect , Improve the effect of anti-pollution ability

Active Publication Date: 2015-07-08
浙江红叶园艺有限公司
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  • Abstract
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AI Technical Summary

Problems solved by technology

In the process of inducing callus, the browning phenomenon is more serious. After adding ordinary antioxidants, slight browning still occurs, and the occurrence of browning cannot be completely inhibited.
In the process of beech shoot tip cultivation, the common MS in the market is used as the basic medium, which is prone to nitrogen deficiency symptoms, and the death rate is high, and the induction rate of adventitious buds is not high.
[0004] At this stage, the research on beech is still limited to the laboratory stage, and there are still many unsolved problems, and it has not been put into factory production, because the application of tree tissue culture in actual production is still subject to many restrictions. for two reasons
[0005]One is that the growth rate of forest trees is relatively slow, and secondary metabolites accumulate a lot, and browning and vitrification often occur in the tissue culture process;
[0006] Second, the effective rate of plant tissue culture regenerated plants is low, making the propagation technology difficult to meet the needs of actual production. The biological characteristics of zelkova determine its growth rate. Slow, it is difficult to directly obtain explants, and the pollution rate is not easy to control. If seeds are used as explants, there may be certain advantages, but the collection of seeds and the possible problems of germination size and age also bring some problems
[0008] In order to solve the problem of low callus differentiation into adventitious buds, it is urgent to screen and discover more suitable medium and induction methods

Method used

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  • Cutting propagation method of strengthening antipollution factor zelkova schneideriana

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The young branches of beech trees are collected near urban areas and other cities affected by smog weather. Observe the trees that are growing well and collect branches that do not affect the growth of beech trees. The following operations are performed:

[0029] 1) Use 2-3-year-old beech branches as cuttings, disinfect the incision with 75% alcohol for 18 seconds, rinse with deionized water, soak in 0.08% nano-silver titanium solution for 10 minutes, and then rinse with deionized water 3 times, dry the water with filter paper for later use;

[0030] 2) Cut the branches treated in step 1) into 1.5cm stems containing at least one axillary bud. The laboratory controls the cultivation environment at normal temperature and pressure, and the air humidity is 90%. On the first day, the clustered buds were obtained, and the clustered buds were put forward for the last 3 days, and the control humidity was 75%. Among them, the formula of cluster bud induction medium is: ...

Embodiment 2

[0045] The third-generation seedlings obtained in Example 1 were washed with clean water and transplanted into a nutrient bowl. The nutrient soil in the nutrient bowl was mixed with plant ash and humus soil at a ratio of 1:5, and a little humus beech leaves were added as the substrate. , add water to moisturize, control light intensity to 1500Lx, sunlight for more than 8 hours, control humidity to 75%, after 5 days of treatment, put the nutrient bowl into the greenhouse for normal cultivation, check the survival rate of this batch of seedlings after 1 month, Up to 95.6%.

Embodiment 3

[0047] The third-generation seedlings obtained in Example 1 were selected to be planted in the open air at the Hengfan red leaf gardening planting base in Lin'an, and the sunny plots with more than 8 hours of sunlight were used to dig pits in the soil for planting, and the ratio of plant ash to humus in the pits Mix it at a ratio of 1:5, add a little humus beech leaves as the substrate, and use automatic sprinkler irrigation facilities for irrigation. After one month, check the survival rate of seedlings is 88%.

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Abstract

The invention belongs to the technical field of plant propagation, in particular to a cutting propagation method of strengthening antipollution factor zelkova schneideriana. Branches of the zelkova schneideriana with the tree age of 2 to 3 are utilized as spike strips, cuts are disinfected by alcohol, flushed by deionized water, soaked by a nano-silver titanium solution for 5-10 minutes, flushed by the deionized water again and reserved for use after moisture is sucked. Branches after treatment is cut to be stem sections, the stem sections are planted into a culture medium for culturing for 25 to 30 days, and multiple shoots are obtained. Big and robust shoots are transferred to a root culture media, and culture seedlings are obtained. The culture seedlings are arranged in a greenhouse for 5 to 7 days, the seedlings are cleaned, cultured and transplanted into humus, the culture seedlings with good growth conditions are screened out for secondary-generation culture and continuous optimizing, and seedlings comes out in a third generation. Rooting percentage of the zelkova schneideriana in cutting propagation is improved, rapid expanding propagation of fine breeds of the zelkova schneideriana is promoted, and resistance of the seedlings of the zelkova schneideriana is effectively improved.

Description

technical field [0001] The invention belongs to the technical field of plant propagation, and in particular relates to a cutting propagation method of beech trees with enhanced anti-pollution factors. Background technique [0002] Beech is an arbor tree species belonging to the genus Beech in the family Ulmaceae. It is a national second-class key protected plant and is a precious hard broad-leaved tree species. The beech tree is hard and fragrant, with purple-red heartwood, dense texture, beautiful color, strong pressure resistance, water and humidity resistance, corrosion resistance, and no cracking. It is one of the precious hard broad-leaved timber species in my country. The precious material of furniture is deeply loved by the masses. At present, there are not many large-scale cultivation of beech trees in China, but the market demand for beech trees is very large, so the large-scale cultivation and reproduction of beech trees is imminent, and it also complies with the n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G1/00
Inventor 余鸿钧丁青松蒋寿松刘霞
Owner 浙江红叶园艺有限公司