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Preparation method and application of apocynum venetum extract

A technology of apocynum extract and alcohol extract, which is applied in the biological field, can solve problems such as no mechanism of action, and achieve the effect of inhibiting the growth of tumor cells, clear mechanism of action, and significant efficacy and activity

Active Publication Date: 2013-06-05
XINJIANG ALTAY PREFECTURE GAUBAO KENDER CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the Apocynum preparations invented in the above patents are based on the traditional medicinal efficacy of Apocynum, and do not involve the mechanism of action

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] a. 5 kg of Apocynum apocynum leaves were crushed, passed through a 20-mesh sieve, extracted with 30% ethanol at room temperature, the extraction time was 3 hours, extracted once, filtered, concentrated under reduced pressure until no alcohol smell, and obtained alcohol extract;

[0024] b. After diluting the alcohol extract obtained in step a with water to contain crude drug 0.2g / ml, degrease with petroleum ether once, wherein the volume ratio of petroleum ether and alcohol extract aqueous solution is 1:1;

[0025] c, the degreasing aqueous solution obtained in step b is ethyl acetate as a solvent, extracting once, the volume ratio of ethyl acetate and degreasing aqueous solution is 1:1, the extract is decompressed and the solvent is recovered, and dried to obtain Apocynum apocynum Extract 60g.

Embodiment 2

[0027] a. 5 kg of Apocynum apocynum leaves are crushed, passed through a 20-mesh sieve, and extracted under reflux with 50% ethanol at an extraction temperature of 60°C for 2 hours, extracted 3 times, filtered, combined extracts, and decompressed Concentrate until there is no alcohol smell to obtain alcohol extract;

[0028] b. After diluting the alcohol extract obtained in step a with water to contain crude drug 1g / ml, degrease 2 times with petroleum ether, wherein the volume ratio of petroleum ether and alcohol extract aqueous solution is 1:2;

[0029] c, the degreasing aqueous solution obtained in step b is extracted twice with n-butanol as a solvent, the volume ratio of n-butanol and degreasing aqueous solution is 1:2, the extract is decompressed and the solvent is recovered, and dried to obtain the apocynum extract Material 250g.

Embodiment 3

[0031] a. After crushing 5 kg of apocynum leaves, passing through a 20-mesh sieve, heating and refluxing with 60% ethanol for extraction, the extraction temperature is 75°C, and the extraction time is 3 hours. Extract twice, filter, combine the extracts, and decompress Concentrate until there is no alcohol smell to obtain alcohol extract;

[0032] b. After diluting the alcohol extract obtained in step a with water to contain crude drug 3g / ml, degrease 4 times with petroleum ether, wherein the volume ratio of petroleum ether and alcohol extract aqueous solution is 1:3;

[0033] c, the water liquid after the degreasing obtained in step b is extracted 4 times with ethyl acetate as a solvent, the volume ratio of ethyl acetate and degreasing aqueous solution is 1:3, the extract is decompressed and the solvent is recovered, and dried to obtain the apocynum extract Material 70g.

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Abstract

The invention discloses a preparation method and application of an apocynum venetum extract. The method comprises the following steps of: crushing apocynum venetum leaves, carrying out reflux extraction at room temperature or heated temperature by using ethanol, filtering, merging extracting solutions, carrying out vacuum concentration until no ethanol smell exists to obtain an ethanol extract; and diluting the ethanol extract by using water, degreasing by using petroleum ether, extracting the ethanol extract by using ethyl acetate or n-butyl alcohol as a solvent, recovering the solvent at reduced pressure, and drying to obtain the apocynum venetum extract. Proved by tests, the apocynum venetum extract obtained by using the method disclosed by the invention is an extract capable of inhibiting the activity of fatty acid synthase so as to be a natural, plant-sourced and nontoxic fatty acid synthase inhibitor.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method of apocynum extract and its use in inhibiting the activity of fatty acid synthase. Background technique [0002] Fatty acid synthase (FAS) is the most important enzyme in the process of fat synthesis in biological cells. Research on poultry FAS by Tian Weixi and others found that there is a high positive correlation between the fat level in the abdominal cavity of poultry and the activity of FAS, and put forward the theory that controlling the activity of FAS is an effective method to control the body fat level of animals (Tian Weixi, 1994. Body fat level of animals and the regulation of fatty acid synthase activity. The Chemistry of Life, 14,(1):184; Tian Weixi et al., 1996. The relationship between body fat level and liver fatty acid synthase activity of laying hens in different growth stages. Journal of Biochemistry, 12(2 ):234-236; Mei Li et al., 1999. Facto...

Claims

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Application Information

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IPC IPC(8): A61K36/24A61K8/97A61P3/04A61P35/00A23L1/29A23L33/00
Inventor 何飞刘起棠马晓丰田维熙孟繁杰黄景凤王莉
Owner XINJIANG ALTAY PREFECTURE GAUBAO KENDER CO LTD
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