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Method for producing microbe feed probiotics by using white spirit vinasse

A microbial feed and liquor distiller's grains technology, applied in the biological field, can solve the problems of long production process, long time-consuming, easy to be contaminated with bacteria, etc., and achieve the effect of improving digestion and absorption, increasing growth speed, and promoting animal immune function

Active Publication Date: 2013-06-12
QILU UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method requires three-stage fermentation, which takes a long time, and the production process is too long, which is easy to be contaminated with bacteria, resulting in high production costs

Method used

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  • Method for producing microbe feed probiotics by using white spirit vinasse

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 3

[0050] The fermentation liquid of Saccharomyces boulardii in Example 3 was prepared according to existing conventional techniques; the fermentation liquid of Saccharomyces boulardii in Examples 1-2 was prepared as follows:

[0051] a. Take 1 ring of Saccharomyces boulardii strain activated on the slant and insert it into the seed culture medium, and culture it in the shake flask for 24 hours at a temperature of 30° C. and a shake flask rotation speed of 180 rpm to obtain a seed solution;

[0052] b. Inoculate the seed solution in the fermentation medium at an inoculation amount of 5% by weight, and cultivate it for 24 hours at a temperature of 30° C. and a rotating speed of 200 rpm to obtain a secondary seed solution;

[0053] c. Inoculate the secondary seed liquid flow into the fermentation medium at an inoculum size of 10% by weight, and cultivate for 34 hours at a temperature of 30°C and a rotation speed of 200rpm to obtain 1.0-1.5×10 9 CFU / ml of Saccharomyces boulardii fer...

Embodiment 1

[0075] A method for producing microbial feed probiotics by utilizing distiller's grains, the steps are as follows:

[0076] (1) Add 4 times the weight of water to the distiller's grains, soak at room temperature for 15 minutes, pass through a 20-mesh sieve, and add acid protease solution with a weight of 0.05% of the filtrate to the filtrate. The acid protease solution has an enzyme activity of 50,000 U / ml. Hydrolyze at 45°C for 4 hours, then adjust the pH to 4.0, add 0.5% glucoamylase solution by weight of the filtrate, the enzyme activity of the glucoamylase solution is 100000U / ml, hydrolyze at 60°C for 4 hours to obtain a hydrolyzate, and then concentrate the hydrolyzate to 25% of the original volume to obtain distiller's grains extract;

[0077] (2) Take 1.5×10 960 copies of CFU / ml fermentation broth of Saccharomyces boulardii, the concentration of live bacteria is 3.0×10 9 20 copies of CFU / ml Lactobacillus plantarum (Lactobacillus plantarum) fermentation broth, the conc...

Embodiment 2

[0083] The method for utilizing liquor distillers grains to produce microbial feed probiotics as described in Example 1, the difference is that,

[0084] In step (2), 50 parts of fermented liquid of Saccharomyces bradii, 30 parts of fermented liquid of Lactobacillus plantarum, and 20 parts of fermented liquid of Bacillus licheniformis.

[0085] The prepared microbial feed probiotic was tested, and the number of live bacteria of Brad's yeast was >1.0×10 9 CFU / g, Lactobacillus plantarum >1.6×10 9 CFU / g, Bacillus licheniformis >6.0×10 8 CFU / g.

[0086] Example 3

[0087] The method for utilizing liquor distillers grains to produce microbial feed probiotics as described in Example 1, the difference is that,

[0088] In step (2), 50 parts of fermented liquid of Saccharomyces bradii, 25 parts of fermented liquid of Lactobacillus plantarum, and 25 parts of fermented liquid of Bacillus licheniformis.

[0089] The prepared microbial feed probiotic was tested, and the number ...

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Abstract

The invention relates to a method for producing microbe feed probiotics by using white spirit vinasse. The method comprises the following steps of: (1) adding water in the vinasse, sieving, adding an acid proteinase solution, hydrolyzing, regulating the pH value, adding a glucoamylase solution, hydrolyzing and concentrating to prepare a vinasse extracting solution; (2) mixing Bullard saccharomycetes fermentation liquor, lactobacillus plantarum and bacillus licheniformis fermentation liquor to prepare a mixed liquor, and separating thallus in the mixed liquor to prepare a mixed wet thallus; (3) preparing a protecting liquor and a compound carrier; and (4) uniformly mixing the mixed wet thallus with the protecting liquor, then adding the vinasse extracting solution and the compound carrier, and drying after uniform mixing to prepare the microbe feed probiotics. According to the method, the microbe feed probiotics are prepared by using the vinasse as the main material, adding a proper amount of nutrient substances and cultivating three probiotics in the liquid form, so that the additional value of the vinasse is improved; and the vinasse is processed by a microbe fermenting method, so that the problem of utilization of the vinasse is solved.

Description

technical field [0001] The invention relates to a method for producing microbial feed probiotics by using liquor distiller's grains, and belongs to the technical field of biotechnology. Background technique [0002] In recent years, the annual output of liquor in my country is about 9 million tons, and the annual output of by-product distiller's grains is about 15 million tons. Liquor distiller's grains are the largest by-product of liquor production, in which nutrients such as unutilized starch, enriched protein, metabolite amino acids, vitamins, mineral elements, and microbial cells remain. As shown in Table 1, these nutrients can be Carry out multi-faceted comprehensive utilization. [0003] Table 1 Liquor distiller's grain components [0004] [0005] Now the country's annual feed grain accounts for about 23% of the country's total grain output. The liquor brewing industry consumes more than 20 million tons of grain every year, and the feed grain saved by producing ...

Claims

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Application Information

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IPC IPC(8): A23K1/06A23K10/38
CPCY02P60/87
Inventor 王瑞明赵殿臣杜新勇王腾飞李丕武范志勇
Owner QILU UNIV OF TECH
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