Method for producing microbe feed probiotics by using white spirit vinasse

A microbial feed and liquor distiller's grains technology, applied in the biological field, can solve the problems of long production process, long time-consuming, easy to be contaminated with bacteria, etc., and achieve the effect of improving digestion and absorption, increasing growth speed, and promoting animal immune function

Active Publication Date: 2013-06-12
QILU UNIV OF TECH +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method requires three-stage fermentation, which takes a long time, and the production proces

Method used

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  • Method for producing microbe feed probiotics by using white spirit vinasse

Examples

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Example Embodiment

[0050] The fermentation broth of Saccharomyces boulardii in Example 3 was prepared according to existing conventional techniques; the fermentation broth of Saccharomyces boulardii in Examples 1 and 2 was prepared as follows:

[0051] a. Take a loop of Brady yeast strain activated on the slope and connect it to the seed culture medium, and culture it in the shake flask for 24 hours under the conditions of a temperature of 30°C and a shake flask rotation speed of 180 rpm to prepare a seed liquid;

[0052] b. Inoculate the seed liquid in the fermentation medium with an inoculum of 5% by weight, and cultivate for 24 hours at a temperature of 30°C and a rotation speed of 200 rpm to obtain a secondary seed liquid;

[0053] c. Inoculate the secondary seed liquid stream into the fermentation medium at an inoculum amount of 10% by weight, culture for 34 hours under the conditions of a temperature of 30°C and a rotation speed of 200 rpm, to obtain 1.0~1.5×10 9 CFU / ml Saccharomyces boulardii fer...

Example Embodiment

[0074] Example 1

[0075] A method for producing microbial feed probiotics by using white wine distiller’s grains, the steps are as follows:

[0076] (1) Add 4 times the weight of water to the distiller’s grains, soak for 15 minutes under stirring at room temperature, pass a 20-mesh sieve, add an acid protease solution of 0.05% by weight of the filtrate to the filtrate, the enzyme activity of the acid protease solution is 50000U / ml Hydrolyze at 45℃ for 4h, then adjust the pH to 4.0, add 0.5% glucoamylase solution by weight of filtrate, the enzyme activity of the glucoamylase solution is 100000U / ml, hydrolyze at 60℃ for 4h to prepare the hydrolysate, and then concentrate the hydrolysate to 25% of the original volume, the distiller's grain extract is prepared;

[0077] (2) Take 1.5×10 9 CFU / ml of Saccharomyces boulardii (Saccharomyces boulardii) fermentation broth 60 parts, the concentration of viable bacteria is 3.0×10 9 CFU / ml of Lactobacillus plantarum (Lactobacillus plantarum) fer...

Example Embodiment

[0082] Example 2

[0083] The method for producing microbial feed probiotics using white wine distiller’s grains as described in Example 1, the difference is that:

[0084] In step (2), 50 parts of brad yeast fermentation broth, 30 parts of Lactobacillus plantarum fermentation broth, and 20 parts of Bacillus licheniformis fermentation broth.

[0085] The prepared microbial feed probiotics were tested, and the number of Brady yeast viable bacteria was >1.0×10 9 CFU / g, Lactobacillus plantarum>1.6×10 9 CFU / g, Bacillus licheniformis>6.0×10 8 CFU / g.

[0086] Example 3

[0087] The method for producing microbial feed probiotics using white wine distiller’s grains as described in Example 1, the difference is that:

[0088] In step (2), 50 parts of Brady yeast fermentation broth, 25 parts of Lactobacillus plantarum fermentation broth, and 25 parts of Bacillus licheniformis fermentation broth.

[0089] The prepared microbial feed probiotics were tested, and the number of Brady yeast viable b...

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Abstract

The invention relates to a method for producing microbe feed probiotics by using white spirit vinasse. The method comprises the following steps of: (1) adding water in the vinasse, sieving, adding an acid proteinase solution, hydrolyzing, regulating the pH value, adding a glucoamylase solution, hydrolyzing and concentrating to prepare a vinasse extracting solution; (2) mixing Bullard saccharomycetes fermentation liquor, lactobacillus plantarum and bacillus licheniformis fermentation liquor to prepare a mixed liquor, and separating thallus in the mixed liquor to prepare a mixed wet thallus; (3) preparing a protecting liquor and a compound carrier; and (4) uniformly mixing the mixed wet thallus with the protecting liquor, then adding the vinasse extracting solution and the compound carrier, and drying after uniform mixing to prepare the microbe feed probiotics. According to the method, the microbe feed probiotics are prepared by using the vinasse as the main material, adding a proper amount of nutrient substances and cultivating three probiotics in the liquid form, so that the additional value of the vinasse is improved; and the vinasse is processed by a microbe fermenting method, so that the problem of utilization of the vinasse is solved.

Description

technical field [0001] The invention relates to a method for producing microbial feed probiotics by using liquor distiller's grains, and belongs to the technical field of biotechnology. Background technique [0002] In recent years, the annual output of liquor in my country is about 9 million tons, and the annual output of by-product distiller's grains is about 15 million tons. Liquor distiller's grains are the largest by-product of liquor production, in which nutrients such as unutilized starch, enriched protein, metabolite amino acids, vitamins, mineral elements, and microbial cells remain. As shown in Table 1, these nutrients can be Carry out multi-faceted comprehensive utilization. [0003] Table 1 Liquor distiller's grain components [0004] [0005] Now the country's annual feed grain accounts for about 23% of the country's total grain output. The liquor brewing industry consumes more than 20 million tons of grain every year, and the feed grain saved by producing ...

Claims

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Application Information

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IPC IPC(8): A23K1/06A23K10/38
CPCY02P60/87
Inventor 王瑞明赵殿臣杜新勇王腾飞李丕武范志勇
Owner QILU UNIV OF TECH
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