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Rabdosia rubescens stem aqueous extract and its preparation method, finger-prints and use as anti-tumor agent

A technology of fingerprint spectrum and Rubescens rubescens, which is applied in the field involving the water extract, and can solve the problems that the effect is not related to the fingerprint spectrum, and has no anti-tumor effect.

Inactive Publication Date: 2013-06-12
BEIJING YISHENG KANGHUA PHARMA TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the therapeutic effect of the water extract of the leaves of Radix japonicus on esophageal cancer, cardia cancer, liver cancer and breast cancer has been studied, these effects have not been associated with fingerprints
All the reported anti-tumor effects are concentrated on the water extract of the leaves of Iris sativa, but not the water extract of the stem of Iris oriolis, and there is no report on the association between the water extract of the stem of oridonium and its fingerprint.

Method used

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  • Rabdosia rubescens stem aqueous extract and its preparation method, finger-prints and use as anti-tumor agent
  • Rabdosia rubescens stem aqueous extract and its preparation method, finger-prints and use as anti-tumor agent
  • Rabdosia rubescens stem aqueous extract and its preparation method, finger-prints and use as anti-tumor agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] The preparation of embodiment 1 Stem Water Extract

[0013] Wash 40g of dried Radix japonicus stems with tap water, dry them in air, cut them into about 1cm long stems, add 1000-1800mL of hot distilled water at a temperature of 70-90°C, and infiltrate, preferably add 1200mL of hot steamed water at a temperature of 80°C Filtration with water. The outflow rate of the leachate is 10-30 mL / min, preferably 20 mL / min. The effluent was concentrated under reduced pressure at 40-60° C., preferably at 50° C., to obtain 3 g of water extract of Rubescens stalks as a light yellow solid. Grind into powder for later research use.

Embodiment 2

[0014] Example 2 HPLC-(-)ESI-Q-TOF-MS / MS fingerprints and HPLC-(+)ESI-Q-TOF-MS / MS fingerprints of Radix Grass Stem Water Extract

[0015] HPLC: Waters 2795Separations Module;

[0016] Detector: Waters 2489Dual absorbance detector;

[0017] Kromasil C 18 (Dikma) reversed-phase column (5_μm, 250_mm_×_4.6_mm);

[0018] Guard column (5_μm, 10_mm_×_4.6_mm);

[0019] Mobile phase: acetonitrile / water (containing 0.5% and 0.05% glacial acetic acid);

[0020] Mobile phase flow rate: 0.3ml / min;

[0021] Injection volume: 50μl;

[0022] Elution method: gradient elution (see Table 1 for specific gradient).

[0023] Table 1 Gradient table of mobile phase for HPLC-analysis of water extract of Bossium sativa a

[0024]

[0025] a A=acetonitrile, B=water (containing 0.05% glacial acetic acid, v / v), C=water (containing 0.5% glacial acetic acid, v / v)

[0026] The ion current fingerprints of the water extract of the stem of Rubescens japonicus under the detection of HPLC-(-)ESI-Q-TO...

Embodiment 3

[0027] The (-)ESI-Q-TOF-MS ion current fingerprint of embodiment 3 Stem water extract

[0028] (-)ESI-Q-TOF-MS: using an optimized small molecule analysis method, the specific parameters are a) Source Type: ESI; b) Ion Polarity: Negative; c) Scan begin: 50m / z; d) Scan begin: 1500m / z; e) Set Capillary: 3500V; f) Set Nebulizer: 0.8Bar; g) Set Dry Heater: 200℃; h) Set DryGas: 8.0ml / min; i) Funnel 1RF: 300Vpp; j) Funnel 2RF: 300Vpp; k) ISCID Energy: 0eV; l) Hexapole RF: 200Vpp; m) Ion Energy: 5eV; n) Low Mass: 200m / z; o) Collision Energy: 14eV; p) Collision RF: 140) Vpp; q) Transfer Time: 110.8μS; r) Pre Puls Storage: 8.7μS; s) Source: 1400v, 2nA; t) Calibrate the mass spectrometer with sodium formate before each analysis.

[0029] Applying the well-explored high-performance liquid chromatography conditions and the system-optimized mass spectrometry conditions, the (-)ESI-Q-TOF-MS analysis of the water extract of the Stem of Ornithus sativa was carried out by using the negative i...

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Abstract

The invention relates to rabdosia rubescens stem aqueous extract and its preparation method and finger-prints, wherein the rabdosia rubescens stem aqueous extract is extracted from rabdosia rubescens stems by distilled water at a temperature of 80DEG C and the finger-prints comprise a HPLC-(-)ESI-Q-TOF-MS / MS finger-print and a HPLC-(+)ESI-Q-TOF-MS / MS finger-print. The invention also relates to the anti-tumor activity of the rabdosia rubescens stem aqueous extract in a S180 mice model and a use of the rabdosia rubescens stem aqueous extract as an anti-tumor agent.

Description

field of invention [0001] The present invention relates to the preparation of a water extract by extracting the stems of Radix Orbis at 80°C with distilled water, the HPLC-(-)ESI-Q-TOF-MS / MS fingerprint of the water extract, and the HPLC-(+ ) ESI-Q-TOF-MS / MS fingerprint, and further relate to the antitumor activity of the water extract on the S180 mouse model and its application as an antitumor agent. The invention belongs to the field of biomedicine. Background technique [0002] Malignant tumors seriously threaten human health. According to the forecast data released by WHO, the incidence of cancer worldwide will increase by 50% in 2020, and the number of new cancer patients worldwide will reach 15 million each year. Although a sufficient number of antineoplastic drugs are available in the hospital bed, they are unsatisfactory because of toxic and side effects. In the study of anticancer drugs, the search for natural anticancer drugs is of great research interest. Of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/53G01N30/08G01N30/88A61P35/00A61K135/00
Inventor 彭师奇赵明姚兴河彭莉
Owner BEIJING YISHENG KANGHUA PHARMA TECH CO LTD