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Orange cabbage carotenoid extracting and measuring method

A technology of carotene and determination method, which is applied in the field of carotenoid extraction, and can solve problems such as no carotenoid extraction process report yet

Inactive Publication Date: 2013-06-12
NORTHWEST A & F UNIV
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  • Abstract
  • Description
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Problems solved by technology

According to the data retrieval done by the applicant, there is no report on the best extraction process of carotenoids in Chinese cabbage [12]

Method used

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  • Orange cabbage carotenoid extracting and measuring method
  • Orange cabbage carotenoid extracting and measuring method
  • Orange cabbage carotenoid extracting and measuring method

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Embodiment Construction

[0044] It should be noted that in the following examples, documents related to the present application will be cited.

[0045] 1. Materials

[0046] The orange Chinese cabbage Jinguan 1 was selected as the seed material (Zhang Lugang, Hui Maixia, Zhang Mingke. Breeding of a new color Chinese cabbage variety "Jinguan 1" [J]. Northern Horticulture, 2005, 04: 67-68+97), Jinguan 2 (Zhang Lugang, Hui Maixia, Zhang Mingke. Breeding of a new color Chinese cabbage variety "Jinguan 2" [J]. Northwest Agricultural Journal, 2007, 01: 204-206), 11J11 (formerly known as 01S915, Jinguan The parent of No. 2 Chinese cabbage was published in "Journal of Northwest Agricultural Science" 2007, No. 01, page 205, Zhang Lugang, Hui Maixia, Zhang Mingke. Breeding of a new color Chinese cabbage variety "Jinguan No. 2" [J]: 204- 206); ordinary Chinese cabbage Qinbai 2 (Ke Guilan, Zhao Limin. Breeding of Qinbai 2 Chinese cabbage [J]. Shaanxi Agricultural Science, 1990, 04: 3-4), Qiuzao 60 (Zhang Lugang,...

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Abstract

The invention discloses an orange cabbage carotenoid extracting and measuring method. The method includes the steps of preparing an extraction solution by ethanol and acetone with 1 to 1 of volume ratio, wherein extraction effect is the best when digestion is conducted for 50 minutes under the conditions of 1 to 20 of material liquid ratio (gram per milliliter) and 60 DEG C of temperature, measuring total amount of carotenoid according to a spectrophotometric method, measuring component of the carotenoid according to a reversed-phase high-performance liquid chromatography (RP-HPLC) method, establishing an RP-HPLC measuring method of the cabbage carotenoid, wherein a mobile phase chosen in the measuring process is composed of carbinol and acetonitrile, flow velocity is 1.00 milliliter per minute, column temperature is 30 DEG C, detection wavelength is 440 nanometers, and sample size is 10 microliters, using xanthophylls, lycopene and beta-carotene as master standards, and building a linear equation of measurement of the xanthophylls, the lycopene and the beta-carotene through the RP-HPLC method.

Description

technical field [0001] The invention relates to a method for extracting carotenoids, in particular to a method for extracting and measuring orange Chinese cabbage carotenoids. Background technique [0002] Chinese cabbage [Brassica campestris ssp. pekinensis (Lour.) Olsson] is the most important vegetable in China, and its output and area rank first among all kinds of vegetables. Common Chinese cabbage is rich in Vc, mineral elements potassium, calcium, magnesium, zinc, etc. In addition to the nutrients of ordinary Chinese cabbage, orange Chinese cabbage also contains antioxidant substances such as polyphenols and carotenoids [1] . Carotenoids are one of the important components that make up the color of plant leaves, flowers and fruits [2] , which can only be synthesized by plants or microorganisms, is an essential nutrient for the human body. In plants, carotenoids can help absorb light energy and prevent the damage caused by photooxidation; in animals, carotenoids hav...

Claims

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Application Information

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IPC IPC(8): G01N21/31
Inventor 张鲁刚莫玉楠
Owner NORTHWEST A & F UNIV
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