Method for extracting and purifying microcystic toxins LR and RR by taking cyanobacterial bloom in Dian Lake as raw material

Abstract

The invention provides a method for extracting and purifying microcystic toxins LR and RR by taking cyanobacterial bloom in the Dian Lake as a raw material. The method comprises the following steps of: A. fishing out cyanobacterial bloom in the Dian Lake during an outbreak period by a biological net, and filtering cyanobacterial bloom samples by a filtering sieve; B. performing multigelation treatment on the processed cyanobacterial bloom samples; C. soaking the cyanobacterial bloom samples processed in the step B in an acetic acid solution, furthermore, damaging the cell walls of the cyanobacterial bloom samples by an ultrasonoscope during the soaking process, and centrifuging the cyanobacterial bloom samples to obtain supernatant; D. activating the supernatant to obtain solid phase extraction columns; E. cleaning the solid phase extraction columns after gathering, drying the solid phase extraction columns, and eluting microcystis toxins; F. enabling an eluant from the step E to pass through a small silica gel column, eluting the small silica gel column by carbinol, and adsorbing the microcystis toxins dissolved in the carbinol; G. rotating an evaporator to concentrate an eluant in the step F; H. separating the microcystic toxins LR and RR by a high performance liquid chromatography; and I. purifying. The method can be used for preparing finished products with high purity.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a method for extracting and purifying microcystins LR and RR from Dianchi Lake cyanobacteria as raw materials. Background technique [0002] The eutrophication process of Dianchi Lake began in the late 1970s, entered the 1980s, especially in the 1990s, and the eutrophication became more and more serious. At the end of the 1960s, the water quality of both Caohai and the outer waters was Class II; in the 1970s, it was Class III; in the late 1970s, the water quality began to deteriorate, and the water quality of Caohai became Class V in the 1980s, and it was inferior to Class V in the 1990s, with severe eutrophication In the 1980s, the quality of the offshore water was grade IV, and in the 1990s it was grade V, indicating severe eutrophication. [0003] The eutrophication of lakes leads to the abnormal proliferation of algae and the release of biotoxins. These secondary metaboli...

AI Technical Summary

Problems solved by technology

[0007] 3. Microcystin LR has strong liver toxicity

In terms of raw materials for the preparation of microcystins, laboratory isolation and cultivation of toxin-producing algae are often used as raw materials, but this method increases the production cost on the one hand, and on the other hand cannot carry out large-scale production due to the limitation of raw materials

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