Quick test paper for organic phosphorus and carbamic acid ester type pesticide residues
A technology for the detection of carbamates and pesticide residues, which is applied in the measurement of color/spectral characteristics, material analysis by observing the impact on chemical indicators, and analysis by making materials undergo chemical reactions, which can solve the problem of increasing randomness and Uncertainty, easy to be affected by temperature, long detection time and other problems, to achieve the effect of easy promotion, convenient temperature control and short detection time
Inactive Publication Date: 2015-05-13
QINGDAO UNIV
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[0003] At present, according to the national standard (GB / T 5009.199-2003 rapid detection of organophosphorus and carbamate pesticide residues in vegetables), the widely used pesticide residue rapid detection card is based on the highly sensitive organophosphorus and carbamate pesticides. The quick test card made of cholinesterase and chromogen is not easy to store, and the operation needs to be squeezed and pressed manually, which is difficult, increases randomness and uncertainty, and is easily affected by temperature, and the test takes a long time
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[0023] A test paper for rapid detection of organophosphorus and carbamate pesticide residues, comprising the following manufacturing steps:
[0024] (1) Preparation of thermosensitive microcapsules
[0025] According to the ratio of 2g: 100mL, prepare the mixture of agarose and 0.1mol / L, pH = 7.0 phosphate buffer solution, put it into a pressure cooker and heat it to 0.05KPa, keep it for 15-20min, and naturally cool it to 37℃ under normal pressure, then Add 20ml, 13% NaOH solution dropwise, add 1.5ml xylenol sulfate dropwise, carry out the methylation reaction at a constant temperature of 37°C, and add 0.5mol / L CH 3 COOH, adjust the pH to neutral pH=7.0, add a certain amount of 1U / ml cholinesterase, add sterilized paraffin oil at 37°C to make it evenly dispersed, put it in an ice water bath at 0°C to solidify, 5000r / Min centrifuge to remove the oil phase for 3 min, repeat 3 times, after the oil phase is removed, the temperature-sensitive microcapsules are obtained;
[0026]...
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Abstract
The invention relates to quick test paper for organic phosphorus and carbamic acid ester type pesticide residues. The technical scheme of the quick test paper is as follows: the test paper is made by the temperature-sensitive microcapsule technology; and encapsulated cholinesterase is released in a special temperature clinical region to catalyze indophenol acetate to hydrolyze and develop color; therefore, the pesticide residue condition is determined. The quick test paper is disposable and low in price; a reagent is not needed to prepare and the maintenance work of the analytic instrument is omitted; the test paper is simpler than manual pressing in use and the result determination is clear at a glance without training; the dosage of the reagent is low, the detection time is short and the detection can be completed in 1 minutes; the cholinesterase is stable under the protection of microcapsules and long in storage time; the thermal environment in the range from 37 to 40 DEG C of solution is easy to realize, and the temperature is quite convenient and accurate to control, and insufficient moisture and too low temperature situations can not occur; besides, the quick test paper is convenient for popularization, and is the first choice for places such as farm product market, supermarket, school, hotel, factory and the like to quickly detect pesticide residues.
Description
technical field [0001] The invention relates to a test paper for rapid screening and determination of organophosphorus and carbamate pesticide residues, belonging to the field of pesticide detection. Background technique [0002] Food is the most important thing for the people, and the food safety problem of pesticide use or abuse is increasing day by day. The rapid detection of organophosphorus and carbamate pesticides in vegetables in bazaars, wholesale markets, supermarkets, and production bases can realize pollution-free vegetables. has great significance. Terminal sites, including households, markets, communities, etc., have a common feature that testing should be completed within a relatively short period of time, and there is no need to give quantified values, only to know whether it exceeds the standard. This type of technology should be easy to use, fast and sensitive enough to judge whether the standard is exceeded or not. There is an urgent need to explore a sim...
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IPC IPC(8): G01N21/78G01N21/25
Inventor 郑艺华刘君
Owner QINGDAO UNIV