Semen momordicae extractive as well as preparation method and use thereof
A kind of wood turtle extract and technology of wood turtle, which is applied in the field of medical testing, can solve the problem of anti-tumor activity that has not been seen yet, and achieve the effect of high component purity, reproducibility, and high purity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0033] Example 1. Preparation of the fruit extract of the fruit
[0034] Grind the Chinese medicinal materials of Saccharia chinensis to obtain the middle-grade powder of Sorcus chinensis, take 100g of the middle-aged powder of Sorcus chinensis, extract with 500ml of sodium acetate solution (20mM, pH 5.0) in the refrigerator at 4°C for overnight, centrifuge and freeze-dry the filtrate to obtain the extract (F), preserved in -20 ℃ refrigerator for subsequent use, after dissolving the extract lyophilized powder (F) obtained by extracting the fruit of the tree turtle with water, centrifugal filtration to remove a small amount of insoluble impurities, further separation by chromatography (chromatogram as shown in Fig. figure 1 shown), collect the 26-40min fractions, freeze-dry the combined collections and store them in a -20°C refrigerator for later use.
[0035] The chromatographic conditions are as follows:
[0036] Chromatographic column: YMC-Pack ODS-A (150×10mm, 5μm)
[003...
Embodiment 2
[0045] Example 2. In vitro anti-tumor effect test of the fruit of the fruit
[0046] The SGC7901 cells in the logarithmic growth phase were inoculated in 96-well plates, and divided into blank control group, carboplatin control group and Saccharina seed extract treatment group (0.1, 0.2, 0.4, 0.6, 0.8, 1.0mg / ml), After culturing at 37°C for 72 hours, MTT assay was performed to calculate the inhibition rate of cell proliferation. The results showed that, with the increase of dose, the extract of Saccharina chinensis significantly inhibited the proliferation of SGC7901 cells (such as figure 2 shown).
Embodiment 3
[0047] Example 3. IC of different tumor cells of the fruit extracts F and F3 of the fruit 50 value
[0048] Human gastric cancer cells SGC7901 in logarithmic growth phase, human prostate cancer cells PC-3 and mouse colon cancer cells CT-26 were inoculated in 96-well plates respectively, and divided into blank control group, carboplatin control group and Saccharomyces japonicus extract In the treatment group (0.1, 0.2, 0.4, 0.6, 0.8, 1.0mg / ml), after cultured at 37°C for 48h, 72h, and 96h, the MTT test was carried out, and the inhibition rate of cell proliferation was calculated. The results showed that, with the dose increasing, the extract of Saccharomyces japonicus significantly inhibited the proliferation of various cancer cells (as shown in Table 2).
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More