Respiratory syncytial virus vaccine and preparation method thereof

A technology of syncytial virus and respiratory tract, applied in the field of human respiratory syncytial virus vaccine and its preparation, can solve the problem of no ideal targeted vaccine

Inactive Publication Date: 2013-07-03
SHANGHAI ZERUN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no ide

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0014] From the liquid nitrogen container, take out the Vero cell strain (source, National Institute for the Control of Pharmaceutical and Biological Products), centrifuge at 1000rpm for 10 minutes, discard the original preservation solution, and add MEM nutrient solution containing 10% neonatal bovine serum and 0.20% hydrolyzed milk protein (pH with 7% NaHCO 3 Adjust to 6.8) and culture at 37°C until the cells grow into a single layer, then wash with PBS, digest with trypsin-EDTA, add the above nutrient solution, and subculture once every 5 days at a seeding rate of 1:2 until a batch is obtained. Produce enough cells for use.

[0015] In the above cell culture concentration up to 10 6 When / ml, in the nutrient solution of pH7.4, add human respiratory syncytial virus strain by the M.O.I. rate of 0.05, static adsorption culture 8 days. In the meantime, the maintenance solution was changed once. After the culture period expired, the virus-cell culture suspension was obtained ...

Embodiment 2

[0017] With the aluminum phosphate adjuvant of above-mentioned embodiment 1 method, inactivated virus vaccine and vaccine composition (inactivated virus vaccine+aluminum phosphate adjuvant) are respectively immunized mice, each immunization 0.5ml, 0 day, 7 days respectively immunize two times The second time, after 14 days of observation, blood was collected to detect serum neutralizing antibodies and titers.

[0018] Table 1

[0019] Vaccine Type

[0020] Therefore, it is shown that the aluminum phosphate adjuvant can significantly increase the potency of the human respiratory syncytial virus inactivated vaccine, play the effect of enhancing immunity, and can greatly reduce the antigenic amount of the purified stock solution of the human respiratory syncytial virus inactivated vaccine in the preparation of the vaccine , has a good protective effect.

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Abstract

The invention discloses a respiratory syncytial virus vaccine and a preparation method thereof. The vaccine contains membrane proteins obtained by cracking of whole virus particles of respiratory syncytial virus, wherein the respiratory syncytial virus vaccine contains 100-200 Mug of viral proteins per dose and 0.5 mg/dose of aluminum phosphate adjuvant. Compared with the existing viral purified stock solution, the respiratory syncytial virus vaccine for human, disclosed by the invention, has a greatly-reduced content of foreign proteins, is better in immunogenicity and potency, is safer in use, and is more suitable for large-scale production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a human respiratory syncytial virus vaccine and a preparation method thereof. Background technique [0002] Respiratory syncytial virus is a single-stranded negative-sense non-segmented RNA virus belonging to the family Myxoviridae and the genus Pneumovirus. The inner core is RNA wrapped by capsid proteins, and the outer layer is coated with host-derived lipid bilayers and transmembrane proteins. The virus mainly encodes 11 kinds of proteins, and three transmembrane proteins are related to immune protection and virulence, namely fusion protein F, adhesion protein G, and small hydrophobin SH. [0003] Respiratory syncytial virus (Respiratory syncytial virus, RSV) is one of the main causes of lower respiratory tract infections in infants and young children worldwide. All serious. Virtually all children become infected by the age of two years, and reinfection occurs more ...

Claims

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Application Information

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IPC IPC(8): A61K39/155A61P31/14
Inventor 田平生丛薇陈明杰
Owner SHANGHAI ZERUN BIOTECHNOLOGY CO LTD
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