Sorbose dehydrogenase and sorbosone dehydrogenase, and applications thereof
The technology of sorbose dehydrogenase and sorbone dehydrogenase is applied in the field of microbial biology and can solve the problems of long cycle, increased process control, nutrient composition of fermentation medium and energy waste, etc.
Inactive Publication Date: 2013-07-03
INST OF BIOENG ACAD OF MILITARY MEDICAL SCI OF THE CHINESE
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Problems solved by technology
The invention provides sorbose dehydrogenase and sorbosone dehydrogenase for gluconobacter oxydans CGMCC No. 1. 637, amino acid sequences of the sorbose dehydrogenase and sorbosone dehydrogenase are shown as SEQ ID No. 2 and SEQ ID No. 4. The invention also provides genes or gene clusters for coding the above proteins. SNDH-SDH gene clusters with self-controlling sequences are connected to broad host range plasmids pBBRiMCS2, and are introduced to the gluconobacter oxydans 621H through conjugative transfer, so that transformation from the sorbose to keto-gulonic acid can be realized.
BacteriaMicroorganism based processes +4
Gene clusterBiology +8
- Experimental program(5)
Description & Claims & Application Information
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