Ratio-dependent florescent probe for identifying Zn<2+> and S<2-> in relayed manner as well as synthesis method and application of ratio-dependent florescent probe
A technology of fluorescent probes and synthesis methods, applied in chemical instruments and methods, fluorescence/phosphorescence, luminescent materials, etc., can solve the problems of fluorescence enhancement, environmental hazards, quenching, etc., and achieve simple product separation and purification process and avoid external factors The effect of accurate detection
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Embodiment 1
[0041] (1) Synthetic relay recognition Zn 2+ and S 2- The reaction formula of the ratiometric fluorescent probe:
[0042]
[0043] (2) Synthetic relay recognition of Zn 2+ and S 2- The specific steps of the ratiometric fluorescent probe:
[0044] Weigh 300mg of the intermediate N -(2-(1H-Benzimidazolyl)phenyl)-2-chloroacetamide (compound 2) and 418mg of raw material bis-(pyridylmethylene)amine (compound 3), dissolved in 20ml of N,N - In dimethylformamide (DMF), stir the reaction for 7 hours at room temperature, add 100ml of distilled water, adjust the pH value to 6 with dilute hydrochloric acid, collect the resulting precipitate by filtration, and recrystallize with acetonitrile to obtain a fluorescent probe N -(2-(1H-Benzimidazolyl)phenyl)-2-bis(2-pyridinemethylamino)acetamide (Compound 1), the yield was 56%.
Embodiment 2
[0046] Weigh 300mg of the intermediate N -(2-(1H-Benzimidazolyl)phenyl)-2-chloroacetamide and 630mg of bis-(pyridylmethylene)amine were dissolved in 20ml of DMF, stirred and reacted for 9h at room temperature, Add 150ml of distilled water, adjust the pH to 7 with dilute hydrochloric acid, collect the resulting precipitate by filtration, and recrystallize with acetonitrile to obtain a fluorescent probe N -(2-(1H-Benzimidazolyl)phenyl)-2-bis(2-pyridinemethylamino)acetamide, the yield is 68%.
Embodiment 3
[0048] Weigh 300mg of the intermediate N -(2-(1H-Benzimidazolyl)phenyl)-2-chloroacetamide and 835mg of bis-(pyridylmethylene)amine were dissolved in 20ml of DMF, and stirred at room temperature for 10h, Add 150ml of distilled water, adjust the pH to 7 with dilute hydrochloric acid, collect the resulting precipitate by filtration, and recrystallize with acetonitrile to obtain a fluorescent probe N -(2-(1H-Benzimidazolyl)phenyl)-2-bis(2-pyridinemethylamino)acetamide, the yield was 72%.
[0049] The fluorescent probe of embodiment 1~embodiment 3 N Basic data for -(2-(1H-benzoimidazolyl)phenyl)-2-bis(2-pyridinemethylamino)acetamide:
[0050] Melting point: 177℃~179℃;
[0051] 1 H NMR (400 MHz, DMSO- d 6 ) δ 13.20 (s, 1H), 13.09 (s, 1H), 8.72 (d, J = 8.4 Hz, 1H), 8.41 (d, J = 4.3 Hz, 2H), 8.04 (d, J = 7.8 Hz, 1H), 7.75 (d, J = 7.8 Hz, 2H), 7.57 (s, 2H), 7.47-7.31 (m, 3H), 7.32-7.04 (m, 5H), 4.13-3.84 (m, 4H), 3.39 (S, 2H). (eg figure 1 ).
[0052] 13 C NMR (100 ...
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