Alkylation derivatization reagent and application of alkylation derivatization reagent in peptide fragment marking and mass spectrometric detection

Abstract

The invention discloses an alkylation derivatization reagent and an application of the alkylation derivatization reagent in peptide fragment marking and mass spectrometric detection. A molecular formula of the alkylation derivatization reagent is brominated 1-[3-[(2-iodine-1-oxo-ethyl) amino] propyl]-3-butyl imidazole (IPBI), and a structure thereof is as shown in Formula (I). The alkylation derivatization reagent takes an iodoacetamide base as an active functional group, and has high hydrophobicity, high gas phase alkalinity and a structural feature of containing permanent positive charges; selective derivatization and efficient mark of active sulfhydryl in polypeptide / protein can be achieved; the charge number of a peptide fragment, the ionization efficiency and the detection sensitivity in mass spectrometric analysis can be increased and improved remarkably, so that a detection rate of a cysteine peptide fragment is increased remarkably; and the alkylation derivatization reagent is simple in synthetic process, lower in cost, good in reaction reproducibility, and convenient to popularize and apply.

Description

technical field

[0001] The invention relates to the labeling of biological macromolecules, in particular to an alkylation derivative reagent and its application in peptide labeling and mass spectrometry detection. Background technique

[0002] In the post-genomics era, proteome analysis has received more and more attention. However, the extreme complexity of protein sample composition, wide dynamic range distribution, and especially the presence of post-translational modifications make its analysis still face great challenges. The bottom-up strategy represented by the shotgun method is the most commonly used strategy for the separation and identification of complex proteome samples. This strategy first digests the protein sample with protease, and the resulting peptide mixture is separated by chromatography and then passed through mass spectrometry. Perform peptide identification. It can be seen that mass spectrometry is an indispensable tool for proteome research. [000...

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