Culture method of cunninghamia lanceolata callus

Abstract

The invention discloses a culture method of cunninghamia lanceolata callus. The culture method comprises the steps of: screening cunninghamia lanceolata seeds with filled particles and without pests as explants, carrying out sterile culture to obtain sterile seedlings, clipping leaves, stems or root systems of the sterile seedlings, inoculating in an induction culture medium for inducing callusogenesis, and finally transferring the callus formed by induction into a proliferation medium for proliferation. The culture method is simple and easy to operate, is capable of effectively shortening the induction and proliferation time of the callus and increasing the induction rate of the cunninghamia lanceolata callus, provides a large quantity of excellent callus materials with good colors and compact structures for the development of the cunninghamia lanceolata industry, and has a good economic benefit.

Description

technical field

[0001] The invention relates to a callus culture method, in particular to a fir callus culture method. Background technique

[0002] Plant callus culture is an important stage in the process of plant tissue culture. Callus can not only induce the formation of complete plants, but also be used for the preservation of germplasm resources, and it is also the source of cells and protoplasts for suspension culture. Chinese fir( Cunninghamia lanceolata ) is a unique coniferous timber tree species in my country. It has good material, fast growth and high yield. It is the main afforestation tree species in southern my country. Chinese fir tissue culture is mainly carried out through axillary bud induction or callus induction, and callus induction is an important link in the process of Chinese fir tissue culture. High induction rate and high-quality callus culture are the key to callus differentiation in the later stage. important basis. Chinese fir tissue culture ...

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