Method for preparing rice protein by utilizing bio-enzyme

A rice protein, biological enzyme technology, applied in the direction of plant protein processing, protein food processing, protein food ingredients, etc., can solve the problems of loss of functional properties, reduced solubility, protein denaturation, etc., to enhance applicability, improve functionality, prolong shelf life effect

Inactive Publication Date: 2015-05-20
合肥锦泰糖业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the long-term high-temperature liquefaction and amylase hydrolysis of rice protein in rice dregs, cross-linking occurs between molecules, and protein denaturation is severe. Maillard reaction (Maillard) makes protein and reducing sugar form N-glycopeptide bonds Combined, the resulting dark pigment makes the rice dregs appear dark yellow in appearance, the starch is partially degraded, and the rice protein is wrapped in the form of extreme dextrin. Therefore, the rice protein in the rice dregs is no longer a natural rice protein, and its solubility is greatly improved. Reduce and lose some functional properties related to it, limiting its application in food

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A kind of method that biological enzyme prepares rice protein, comprises the following steps:

[0022] (1) Soaking: Add the raw rice dregs into 2% sodium bicarbonate aqueous solution which is 6 times its weight and soak for 2 hours;

[0023] (2) Desugaring: add 0.3% glucoamylase by weight of raw rice dregs, add water or acetic acid, adjust pH to 5, and saccharify at 55°C for 3 hours;

[0024] (3) Degreasing: add water or acetic acid, adjust the pH to 7, add 0.2% lipase by weight of raw rice dregs, and degrease at a temperature of 50°C for 6 hours;

[0025] (4) Washing: wash the rice dregs after the above treatment with 2% aqueous sodium bicarbonate solution at 70°C for 4 times;

[0026] (5) Pressure filtration: filter the washed rice dregs through a plate and frame to remove the aqueous solution, the filter medium is a high-temperature shaped filter cloth, and the operating pressure is 0.18MPa;

[0027] (6) Enzymolysis: first add water 10 times the weight of the filte...

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PUM

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Abstract

The invention discloses a method for preparing rice protein by utilizing a bio-enzyme. The method comprises the following steps of: immersion, desugaring, degreasing, washing, press filtering, enzymolysis, enzyme deactivation, debitterizing, press filtering and decoloring, concentration and drying. The fat in rice dregs is decomposed through the immersion, desugaring and degreasing steps, the rancidity phenomenon of the fat in the storing process can be prevented, the shelf life can be prolonged, the rice protein is degraded and trimmed by utilizing a protease, so that the rice protein is converted to soluble peptide so as to be extracted, the functionality of the rice protein is improved, the application performance of the rice protein in food can be enhanced, enzymolyzed protein liquid is concentrated at a low temperature by utilizing a reverse osmosis membrance, then the protein is dried by mist, and the good solubility of a product can be guaranteed.

Description

technical field [0001] The invention mainly relates to a method for preparing rice protein, in particular to a method for preparing rice protein with biological enzymes. Background technique [0002] Rice protein is a kind of high-quality grain protein. Its amino acid composition is balanced and reasonable, which is very close to the nutritional mode recommended by WHO / FAO; the human body has high utilization rate, and the biological value (BV) can be as high as 77. The protein utilization rate is 1.36-2.56%. All kinds of food rank first. More importantly, rice protein is hypoallergenic and highly nutritious, making it very suitable as a protein supplement for infants or special populations. However, the protein content in rice is low, only about 8%, and the starch granules in rice endosperm cells are closely combined with protein, so the technical difficulty of extracting rice protein from rice is relatively large, and the cost is high. There is less research. [000...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23J1/12A23J3/14A23J3/34
Inventor 王金德王金枝王建凯高学庆
Owner 合肥锦泰糖业有限公司
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