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An identification method for EPCs (endothelial progenitor cells) of elite ice and snow athletes

A technology of endothelial progenitor cells and athletes, applied in the field of identification of vascular endothelial progenitor cells, can solve the problems of not being able to distinguish between EPCs and hematopoietic stem cells, and achieve the effect of improving staining efficiency and saving staining time

Inactive Publication Date: 2013-08-07
HARBIN INST OF PHYSICAL EDUCATION
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Problems solved by technology

However, CD133 is a surface marker of hematopoietic stem cells, which is selectively expressed in early hematopoietic cells and progenitor cells of bone marrow, embryonic liver and peripheral blood, so it cannot be used to distinguish EPCs from hematopoietic stem cells, and CD34 and VEGFR-2 , which are shared by hematopoietic and endothelial cells and are not essential markers of EPCs

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  • An identification method for EPCs (endothelial progenitor cells) of elite ice and snow athletes

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Embodiment Construction

[0007] (1) Isolation and culture of peripheral blood CD34+ endothelial progenitor cells of excellent ice and snow athletes

[0008] Aseptically collect 40ml of peripheral blood from elite ice and snow athletes, anticoagulate with heparin, and dilute the blood with Hakn’s solution.

[0009] Then use Fiocll density gradient centrifugation to separate. Methods as below:

[0010] (1) Add lymphocyte separation liquid in the centrifuge tube;

[0011] (2) Take heparin anticoagulant blood and the same amount of Hanks solution and shake well, slowly superimpose on the layered liquid surface along the tube wall with a dropper, pay attention to keep a clear interface, and centrifuge horizontally at 2000rpm for 20min;

[0012] (3) After centrifugation, the tube is divided into three layers, the upper layer is plasma and Hanks solution, and the lower layer is mainly red blood cells and granulocytes. The middle layer is the lymphocyte separation liquid, and at the interface of the upper...

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Abstract

The present invention discloses an identification method for EPCs (endothelial progenitor cells) of elite ice and snow athletes. According to the method of the present invention, a transmission electron microscope is adopted to observe organelle Weibel-Palade bodies to identify the EPCs. The method has high specificity and low cost.

Description

technical field [0001] The invention relates to a method for identifying vascular endothelial progenitor cells, in particular to the identification of vascular endothelial progenitor cells of excellent ice and snow athletes, and belongs to the cross fields of sports medicine, cell biology and biotechnology. Background technique [0002] Ice and snow sports are endurance events, which have high requirements on the functions of the cardiovascular system and respiratory system. Not only requires a good aerobic metabolism function, but also a strong negative oxygen capacity, so the cardiopulmonary function of athletes should be an important factor in the selection of materials. Generally, ice and snow athletes have well-developed cardiopulmonary function, large heart capacity, strong pulse, large stroke output, low pulse activity frequency, large pulse pressure difference, rapid pulse fall, and large pulse volume. As an endothelial cell relaxation factor, nitric oxide is mainly...

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Application Information

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IPC IPC(8): G01N23/22G01N1/30
Inventor 王世君张海龙胡萍白春雨李向臣关伟军
Owner HARBIN INST OF PHYSICAL EDUCATION