Method for enriching gallotannin with antioxidation activity from mangos
An anti-oxidant activity and gallon technology, which is applied in the field of extraction and separation of natural compounds, can solve the problems of no enrichment of gallic tannins and complex structures of tannins, and achieve safe and easy-to-operate production processes, high antioxidant activity, and production short cycle effect
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Embodiment 1
[0017] See figure 1 , To enrich the gallbladder tannins from the mango seeds: Weigh 1.5 g of the freeze-dried powder of Macesu, Tainong No. 1, and Mango fruit seeds respectively, and ultrasonicate for 30 minutes with 80% acetone (solid-liquid ratio 1:20) , Centrifuge the supernatant, repeat twice, combine the supernatants, evaporate to dryness on a rotary evaporator at 45°C and dissolve in 25 ml of water with deionized water. C18 solid phase extraction column (Waters 12cc, 2 g) is first activated with 15 ml methanol, and then equilibrated with 15 ml water, then 25 ml of the extract is loaded, and after sufficient adsorption, it is rinsed with 25 ml of water to remove sugar and acid, etc. Water-soluble impurities are then eluted with 25 ml methanol, 15 ml chloroform is used to elute the remaining pigment and other impurities in the column, and finally the column is regenerated with 15 ml methanol. The methanol eluate was evaporated to dry powder on vacuum drying. The yields wer...
Embodiment 2
[0023] To enrich the gallbladder tannins from mango peels: Weigh 1.5 g of Macesu, Tainong No. 1 and Zihua Mango peel freeze-dried powder respectively, ultrasonicate with 80% acetone (solid-liquid ratio 1:20) for 30 minutes, and centrifuge to obtain The supernatant was repeated twice, and the supernatant was combined, evaporated to dryness on a rotary evaporator at 45 degrees, and dissolved in 25 ml of water with deionized water. C18 solid phase extraction column (Waters 12cc, 2 g) is first activated with 15 ml of methanol, then equilibrated with 15 ml of water, then 25 ml of the extract is loaded, and after sufficient adsorption, it is rinsed with 25 ml of water to remove sugar and acid, etc. Water-soluble impurities are then eluted with 25 ml methanol, 15 ml chloroform is used to elute the remaining pigment and other impurities in the column, and finally the column is regenerated with 15 ml methanol. The methanol eluate was evaporated to dry powder on vacuum drying. The yields...
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