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Application of isorhamnetin to preparing drugs for adriamycin adjuvant therapy

A technology of isorhamnetin and adjuvant therapy, which is applied in the field of medicine and can solve problems such as limiting clinical application and myocardial dysfunction

Inactive Publication Date: 2013-08-14
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the dose-dependent and cumulative myocardial toxicity caused by doxorubicin severely limits its clinical application. Many patients, especially children and adolescents, often develop myocardial dysfunction, cardiomyopathy and severe heart failure several years after stopping chemotherapy.

Method used

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  • Application of isorhamnetin to preparing drugs for adriamycin adjuvant therapy
  • Application of isorhamnetin to preparing drugs for adriamycin adjuvant therapy
  • Application of isorhamnetin to preparing drugs for adriamycin adjuvant therapy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Effects of isorhamnetin on myocardial tissue structure and myocardial enzyme activity induced by adriamycin in rats.

[0022] 1 material

[0023] Isorhamnetin was purchased from Shanghai Ronghe Pharmaceutical Technology Development Co., Ltd.; Adriamycin was purchased from Beijing Cancer Hospital;

[0024] Hematoxylin and eosin were purchased from Beijing Zhongshan Jinqiao Company; creatine kinase (CK), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) assay kits were purchased from Hong Kong Zhongsheng Beikong Biotechnology Co., Ltd.

[0025] SD rats were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.

[0026] 2 methods

[0027] 2.1 Animal husbandry, grouping and handling

[0028] This experimental protocol was carried out in accordance with the Chinese Guidelines for the Application of Laboratory Animals. Rats with a body weight of 300±10g were raised in the SPF feeding room of the Animal Experiment Center of...

Embodiment 2

[0037] Example 2: Effects of isorhamnetin on apoptosis and mitochondrial damage of rat cardiomyocytes induced by doxorubicin.

[0038] 1 Materials: Isorhamnetin was purchased from Shanghai Ronghe Pharmaceutical Technology Development Co., Ltd.; Adriamycin was purchased from Beijing Cancer Hospital; TUNEL kit was purchased from Roche; SD rats were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. .

[0039] 2 methods

[0040] 2.1 Animal feeding, grouping and treatment are the same as in Example 1.

[0041] 2.2 TUNEL staining of myocardial tissue to detect cell apoptosis

[0042] After the rat left ventricle was fixed and sliced, it was deparaffinized in xylene, dehydrated in gradient alcohol, repaired in citrate buffer solution, and washed with PBS for 5 minutes. Blot the moisture around the tissue with 0.3% H 2 o 2 methanol solution for 30 min to inactivate endogenous peroxidase. Then it was incubated with a solution containing 0.1% citrate an...

Embodiment 3

[0047] Example 3: Effects of isorhamnetin on the activity of antioxidant enzymes and the production of lipid peroxidation products in rat myocardial tissue induced by adriamycin.

[0048] 1 Materials: Isorhamnetin was purchased from Shanghai Ronghe Pharmaceutical Technology Development Co., Ltd.; Adriamycin was purchased from Beijing Cancer Hospital; superoxide dismutase (SOD), catalase (CAT), glutathione peroxidation GSH-PX detection kit and malondialdehyde (MDA) detection kit were purchased from Nanjing Jiancheng Technology Co., Ltd.; SD rats were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.

[0049] 2 methods:

[0050] 2.1 Animal feeding, grouping and treatment are the same as in Example 1.

[0051] 2.2 Detection of antioxidant enzyme activity and lipid peroxidation product generation in myocardial tissue

[0052] Take the left ventricle of the rat, weigh it, cut it into pieces, add normal saline, homogenate with an electric homogenizer, c...

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Abstract

The invention discloses new drug application of adriamycin and particularly discloses the application of adriamycin to preparing drugs for the adjuvant therapy of tumor patients taking adriamycin for a long term in clinic.

Description

technical field [0001] The invention relates to the application of a medicinal active ingredient in the preparation of medicine, in particular to the application of isorhamnetin in the preparation of medicine, which belongs to the technical field of medicine. Background technique [0002] Doxorubicin (Doxorubicin) is one of the anthracycline antibiotics. It is one of the most widely and effective anti-tumor drugs in clinical application, and is often used in the treatment of various hematological tumors and solid tumors. However, the dose-dependent and cumulative myocardial toxicity caused by doxorubicin severely limits its clinical application. Many patients, especially children and adolescents, often develop myocardial dysfunction, cardiomyopathy and severe heart failure several years after stopping chemotherapy. . With the increase of tumor survival patients, it is more and more necessary to develop a drug or treatment that can reduce the cardiotoxic side effects of doxo...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P9/00A61P35/00
Inventor 孙晓波孙桂波孙静
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI