Method for preparing salvianolic acid A

A technology of salvianolic acid and acidic substances, applied in the field of preparing high-purity salvianolic acid A, can solve the problems of low extraction rate and low purity of salvianolic acid A, reduce impurity content and purification difficulty, improve conversion rate, and improve The effect of industrial production

Active Publication Date: 2013-08-14
CHENGDU KEYUAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to overcome the low extraction rate and low purity of salvianolic ac...

Method used

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  • Method for preparing salvianolic acid A

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Effect test

Embodiment 1

[0023] Embodiment 1 A kind of method for preparing salvianolic acid A

[0024] A method for preparing salvianolic acid A, comprising the following steps:

[0025] (1) Get the salvianolic acid B extract that the salvianolic acid B content is 2%, take the salvianolic acid B extract weight as a benchmark, add the dimethyl sulfoxide of 7 times of weight parts and dissolve completely, add the acetic acid of 1 times of weight (100% acetic acid concentration), heated at 130° C. for 5 hours, and cooled to obtain a salvianolic acid A enrichment solution;

[0026] (2) The salvianolic acid A enrichment solution obtained in the cooling step (1) is dissolved in water with 3.6 times the weight of the salvianolic acid A enrichment solution, filtered, and the filtrate is separated by HZ841 macroporous resin column chromatography to obtain salvianolic acid a.

[0027] In step (2) of this example, HP-20, XAD-8, HZ801, HZ803, HB16, D1450, HP-20ss or XAD-16 macroporous resin column chromatograp...

Embodiment 2

[0028] Embodiment 2 A kind of method for preparing salvianolic acid A

[0029] A method for preparing salvianolic acid A, comprising the following steps:

[0030] (1) get salvianolic acid B content and be the material containing salvianolic acid B of 10%, add the ethylene glycol of 10 times of weight and dissolve completely, add the acetic acid of 1 times of weight (acetic acid volume concentration is 0.01%), at 110 ℃ Under heating for 5 hours, cooling to obtain the salvianolic acid A enrichment solution;

[0031] (2) The enriched solution of salvianolic acid A obtained in the cooling step (1) was dissolved by adding 5 times the weight of water, filtered, and the filtrate was separated by HP-20 macroporous resin column chromatography to obtain salvianolic acid A.

[0032] In step (2) of this example, XAD-8, HZ801, HZ803, HZ841, HB16, D1450, HP-20ss or XAD-16 macroporous resin column chromatography can also be used for separation.

Embodiment 3

[0033] Embodiment 3 A kind of method for preparing salvianolic acid A

[0034] A method for preparing salvianolic acid A, comprising the following steps:

[0035] (1) Get the salvianolic acid B extract that salvianolic acid B content is 10%, add the dimethyl sulfoxide of 7 times of weight and dissolve completely, add the acetic acid of one time of weight (acetic acid volume concentration is 5%), 112 ℃ of temperature , heated for 5 hours, and cooled to obtain a salvianolic acid A enrichment solution;

[0036] (2) The enriched solution of salvianolic acid A obtained in the cooling step (1) was dissolved by adding 4 times the weight of water, filtered, and the filtrate was separated by HZ841 macroporous resin column chromatography to obtain salvianolic acid A.

[0037] In step (2) of this example, HP-20, XAD-8, HZ801, HZ803, HB16, D1450, HP-20ss or XAD-16 macroporous resin column chromatography can also be used for separation.

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Abstract

The invention discloses a method for preparing salvianolic acid A. The method comprises the following steps of: (1), with a substance containing salvianolic acid B as a material, adding an acid substance with weight 0.1-10 times that of the raw material and concentration of 0.01%(v/v)-100%(v/v); adding an organic solvent with weight 0.01-10 times that of the acid substance and boiling point higher than 100 DEG C; heating for 3-5 hours at the temperature of 110-130 DEG C to obtain salvianolic acid A enrichment liquid, wherein the salvianolic acid B accounts for 2%-100% by weight in the substance containing the salvianolic acid B; and (2), cooling the salvianolic acid A enrichment liquid, adding water which is 2-6 times of the enrichment liquid to dilute and purifying to obtain the salvianolic acid A. The method for preparing salvianolic acid A can be used for preventing the salvianolic acid A from oxidizing in a high-temperature aqueous solution, improving the conversion rate of the salvianolic acid B substance, and achieving the material extracting rate of 20%-60%. Meanwhile, the impurity content and purifying difficulty of the final product salvianolic acid A are lowered, so that the prepared salvianolic acid A has purity higher than 90% and can be used for carrying out industrial production well.

Description

technical field [0001] The invention relates to a method for preparing salvianolic acid A, in particular to a method for preparing high-purity salvianolic acid A. Background technique [0002] Salvia miltiorrhiza Bunge is a perennial herb belonging to the genus Salvia of the Lamiaceae family. Its dried root is a long-standing medicine for promoting blood circulation and removing stasis commonly used in traditional Chinese medicine. Blood, promoting blood circulation and removing blood stasis, nourishing blood and nourishing Qi, dispelling numbness and strengthening feet, regulating menstruation and stopping collapse, detoxification and expulsion of pus". Salvia is often used to treat cardiovascular and cerebrovascular diseases such as angina pectoris, hypertension, coronary heart disease and stroke. There are many traditional Chinese medicine preparations that use Danshen decoction pieces or crude extracts of Danshen as medicine, such as compound Danshen tablets, compound D...

Claims

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Application Information

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IPC IPC(8): C07C69/732C07C67/32
Inventor 汤世江
Owner CHENGDU KEYUAN BIOTECH
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