Novel application of trehalose-montmorillonite nano compound as feed additive
A feed additive, trehalose technology is applied in the new application field of nanocomposite as feed additive, which can solve the problems such as no relevant research reports, and achieve the effects of promoting animal growth, protecting intestinal mucosa and killing harmful intestinal bacteria.
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Embodiment 1
[0014] Example 1: Antibacterial performance experiment of trehalose-montmorillonite nanocomposite
[0015] In this experiment, montmorillonite (M), trehalose (T), and trehalose-montmorillonite nanocomposite (TMNC) were tested on the common Gram-negative bacteria Escherichia coli ( Escherichia. coli , strain source: American Type Culture Collection, number: ATCC25922) and Gram-positive bacteria Staphylococcus aureus ( Staphyloccocus aureus , strain source: American Type Culture Collection, No.: ATCC25923) MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) were studied.
[0016] In this experiment, the two-fold dilution method in test tube broth was used.
[0017] The experimental results are shown in the table below.
[0018]
[0019] It can be seen from the above table that montmorillonite has no antibacterial and bactericidal effects on the two bacteria, trehalose has weak antibacterial properties, and trehalose-montmorillonite nanoco...
Embodiment 2
[0026] Embodiment 2: Trehalose-montmorillonite nanocomposite is used as the broiler test of antibacterial additive for feeding
[0027] The trehalose-montmorillonite nanocomposite was compared with the existing antibiotic additive aureomycin as an antibacterial additive for broiler feed, and the results are as follows.
[0028] deal with body weight / g body weight / g body weight / g material to weight ratio material to weight ratio 1 d 21 d 42 d 1-21 d 1-42 d control group 46.1±0.9 644.5±21.6 a 1805.6±70.2 a 1.57±0.08 a 1.96±0.07 a Control group +1g / kg TMNC 46.3±1.1 680.7±20.8 bc 1882.4±51.6 cd 1.49±0.07 cd 1.84±0.06 cd Control group +2g / kg TMNC 46.2±1.0 693.2±19.4 c 1903.5±62.7 d 1.48±0.05 d 1.82±0.05 d Control group + aureomycin 46.3±0.8 675.1±26.5 bc 1864.7±53.4 bc 1.51±0.06 bc 1.92±0.06 ab
[0029] Note: The letters in the shoulder notes in the table are only used for grouping records d...
Embodiment 3
[0042] Embodiment 3: Trehalose-montmorillonite nanocomposite is used as the piglet test of feeding antibacterial additive
[0043] The piglets described below are all piglets at the weaning stage.
[0044] The trehalose-montmorillonite nanocomposite was used as an antibacterial additive for piglet feed to conduct a comparative study with the existing antibiotic additive aureomycin, and the research results are as follows.
[0045] deal with Average daily gain / g Average daily feed intake / g material to weight ratio control group 415.49±12.55 c 854.92±57.54 2.06±0.12 a Control group +1g / kg TMNC 481.21±16.70 ab 814.25±70.51 1.69±0.08 c Control group +2g / kg TMNC 546.66±13.32 a 861.15±65.42 1.58±0.11 d Control group + aureomycin 469.35±18.69 b 836.49±72.26 1.78±0.07 b
[0046] Note: The letters in the shoulder notes in the table are only used for grouping records during the ex...
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