DBP (dibutyl phthalate) residue detection kit

A technology of dibutyl phthalate and detection kit, which is applied in the measurement of color/spectral characteristics, etc., can solve the problems of insufficient sensitivity, high requirements on instrument conditions, complicated pretreatment operations, etc., to ensure environmental safety and facilitate the The effect of promotion

Inactive Publication Date: 2013-08-21
HUAZHONG NORMAL UNIV
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  • Claims
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Problems solved by technology

[0010] Toyoda et al. used monoclonal antibodies for the first time to use artificial antigen-coated indirect competitive ELISA to detect environmental endocrine disruptors such as DBP, but the detection sensitivity was not high enough, and the lowest detection limit for DBP was >100 μg / L
[0011] At present, the monitoring method for DBP at home and abroad is mainly chromatography, but chromatography has high requirements on instrument conditions, and the pretreatment operation is relatively complicated and time-consuming.
There have been studies on DBP immunoassay based on polyclonal antibodies, but the preparation of polyclonal antibodies is limited by animal immunization time and immunization batches and cannot be produced in unlimited quantities

Method used

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  • DBP (dibutyl phthalate) residue detection kit

Examples

Experimental program
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Embodiment 1

[0036] Determination of DBP content in embodiment 1 daily consumer goods

[0037] 1. Sample pretreatment: cut food preservation bags and disposable plastic cups into 0.5cm 2 Fragments, weigh 0.5g X brand nail polish, weigh 0.5g, add 5mL acetonitrile respectively, and extract overnight in a stoppered glass test tube. Take 3mL of the extract to distill off the acetonitrile under reduced pressure, dissolve the residue with 1-2mL of the competition reaction solution, and store it at -20°C after aliquoting.

[0038] 2. Dilute the coating antigen DBAP-BSA to 0.5 μg / mL with coating buffer, and coat the microtiter plate with 100 μL / well (blank control well A 0 Add 100 μL of coating buffer), overnight at 4°C.

[0039] 3. Wash the ELISA plate 3 times with washing solution, add 250 μL of blocking solution to each well, and incubate at 37°C for 1 hour.

[0040]4. Wash the ELISA plate for 3 times with washing solution, add different concentrations of dibutyl phthalate (gradiently dilute...

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Abstract

The invention provides a DBP (dibutyl phthalate) residue detection kit. The kit comprises DBP standard substance, envelope antigen, envelope buffer solution, a confining liquid, a washing buffer solution, a competition reaction liquid, a DBP monoclonal antibody, an antibody diluent, a goat anti-mouse IgG labeled by avidin, avidin labeled by peroxidase, an OPD (o-phenylenediamine) substrate color development liquid and a stop solution. The kit is low in cost, high in detection speed and high in sensitivity, the limit of detection is up to 6.25ng / mL, and the kit can be used for field rapid screening for large quantity of samples.

Description

technical field [0001] The invention relates to a dibutyl phthalate residue detection kit and a method for detecting dibutyl phthalate residue, belonging to the field of biological detection. Background technique [0002] Dibutyl phthalate (DBP), also known as dibutyl phthalate, is a member of Phthalate (PAEs) compounds. English name Dibutyl phthalate, molecular formula C 16 h 22 o 4 , molecular weight 278.35. Dibutyl phthalate is a colorless, odorless, oily liquid. Vapor pressure 2.7×10 -5 mmHg (25°C), melting point -35°C, boiling point 340°C, flash point 188°C, density 1.042g / cm 3 (20°C). Hardly soluble in water, the solubility in water is 11.2mg / L (20℃). [0003] DBP is an excellent plasticizer for nitrocellulose paint, with strong gelling ability and good softening effect. Its stability, flex resistance, adhesion and water resistance are better than other plasticizers. It can also be used as a plasticizer for polyvinyl chloride (PVC), polyvinyl acetate, alkyd re...

Claims

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Application Information

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IPC IPC(8): G01N21/31
Inventor 赵静云魏晨曦
Owner HUAZHONG NORMAL UNIV
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