333 results about "O-Phenylenediamine" patented technology

The invention discloses a preparation method of an amphiphilic carbon dot, the amphiphilic carbon dot and application of the amphiphilic carbon dot. According to the amphiphilic carbon dots, at least one of phenol, catechol, resorcinol or hydroquinone is used as a first precursor, and at least one of urea, aniline, o-phenylenediamine or 1, 2, 4-triaminobenzene is selected as a second precursor; after mixing and dissolving, reacting for 4-8 hours under the condition of 180-220 DEG C to prepare initial carbon dots; separating and purifying the initial carbon dots in a chromatographic column, collecting a second component, removing the solvent through rotary evaporation, and drying to obtain the amphiphilic carbon dots. The degreasing agent prepared from the obtained amphiphilic carbon dots has strong adsorption capacity, low toxicity and good stability, can effectively remove oil stains, has the characteristic of low foam in the use process, simplifies the cleaning process and improves the efficiency.

The invention relates to a preparation method and application of polysaccharide-based fluorescent carbon quantum dots with adjustable emission wavelength, and belongs to the technical field of preparation of fluorescent carbon nanomaterials. Polysaccharide is respectively mixed with different nitrogen dopants o-phenylenediamine, m-phenylenediamine and p-phenylenediamine, then the mixture is subjected to a hydrothermal reaction to respectively prepare blue, green and yellow green fluorescent carbon quantum dots, and then the blue, green and yellow green fluorescent carbon quantum dots are applied to nitrite detection. The carbon quantum dots with different emission wavelengths and different colors are obtained by adjusting the types of the nitrogen doping agents, the problems that the emission wavelengths of the carbon quantum dots cannot be adjusted and the range is narrow are solved, and reference is provided for subsequent design and opening of the generator-based multicolor fluorescent carbon quantum dots. Meanwhile, the biological polysaccharide is used as a raw material, deionized water is used as a solvent, the whole technological process is easy to operate and low in cost, and the method has far-reaching significance on further high-value utilization of biomass resources and promotion of sustainable utilization of the biomass resources.

The invention provides a colorimetric / fluorescent dual-mode sensor, a preparation method of the colorimetric / fluorescent dual-mode sensor and application of the colorimetric / fluorescent dual-mode sensor in histamine detection. The colorimetric / fluorescent dual-mode sensor comprises a horseradish peroxidase-loaded metal organic framework (HRP-AIE-MOF), hydrogen peroxide and a chromogenic substrate o-phenylenediamine (OPD), horse radish peroxidase (HRP) in the HRP-coated AIE-MOF can oxidize colorless OPD into yellow 2, 3-diaminophenazine (DAP), an ultraviolet absorption peak is generated at 425 nm, a strong fluorescence emission peak is generated at 555 nm, the HRP-coated AIE-MOF has a strong fluorescence emission peak at 450 nm, and the HRP-coated AIE-MOF and a fluorescence signal of the DAP can form a ratio fluorescence sensor. According to the invention, the excellent catalytic ability of natural enzyme and the luminescence characteristic of AIE-MOF are integrated in one system, and high-sensitivity histamine detection is realized.

The invention provides a colorimetric / fluorescent dual-mode nano-enzyme sensor, a preparation method thereof and application of the colorimetric / fluorescent dual-mode nano-enzyme sensor in formaldehyde detection. The sensor comprises a metal organic framework nano-enzyme AIE-MOF coated PCN-222 loaded with an AIE-MOF material, hydrogen peroxide and a chromogenic substrate o-phenylenediamine OPD; the metal organic framework nano enzyme PCN-222 in the AIE-MOF-coated PCN-222 can oxidize colorless OPD into yellow 2, 3-diaminophenazine DAP, an ultraviolet absorption peak is generated at the position of 450 nm, a strong fluorescence emission peak is generated at the position of 560 nm, the AIE-MOF-coated PCN-222 has a strong fluorescence emission peak at the position of 446 nm, and the AIE-MOF-coated PCN-222 and a fluorescence signal of the DAP can form a ratio fluorescence sensor. According to the invention, the excellent catalytic ability of the nano-enzyme and the luminescence characteristic of AIE-MOF are integrated in one system, so that high-sensitivity formaldehyde detection is realized.

The invention belongs to the field of water-based metal ion battery electrode materials, and particularly relates to a quinoneazine organic electrode material with multiple active sites and a preparation method and application of the quinoneazine organic electrode material. The preparation method comprises the following steps: synthesizing dihydroxy phenazine in a nitrogen atmosphere, fully grinding the dihydroxy phenazine and an o-phenylenediamine derivative, sintering in a tubular furnace, cooling and washing to obtain an intermediate; and oxidizing the intermediate under an acidic condition to obtain a product. A plurality of carbon-nitrogen double bonds and carbon-oxygen double bonds exist in the molecular structure of the quinoneazine compound, and redox active sites are rich and are uniformly dispersed. The quinoneazine compound realizes high specific capacity, high material utilization rate, excellent rate capability and cycling stability; meanwhile, according to the quinoneazine compound, the solubility of the compound is reduced through a method of expanding molecules with conjugated structures, and the problem of battery stability caused by the fact that an organic electrode material is easily dissolved in an electrolyte is solved. And the water-based zinc ion battery with the advantages of high capacity, good cycling stability, high energy storage density and the like is constructed.

The invention discloses an ECL detector for detecting trace cesium ions and application of the ECL detector, the ECL detector comprises a working electrode, a reference electrode, a counter electrode, an electrolyte and a photomultiplier, and a poly-o-phenylenediamine film with a porous structure is deposited on the surface of the working electrode. The ECL detector for detecting trace cesium ions provided by the invention has the advantages of ultralow detection limit, high selectivity and field visual detection, and can be successfully applied to visual and accurate determination of trace cesium in various environmental samples such as fresh water, salt water and the like and various animal samples (fishes, arthropods and mollusks).

The invention discloses a preparation method of a sulfur-doped near-infrared carbonized polymer for detecting mercury ions and detection application of the sulfur-doped near-infrared carbonized polymer, and the preparation method is characterized by comprising the following specific steps: dissolving 1-4 mmol of o-phenylenediamine, 1 mmol of trimesic acid and 1-4 mmol of 4-aminothiophenol in deionized water, adding 200-600 mu L of concentrated sulfuric acid, performing ultrasonic treatment, transferring into a polyethylene tetrafluoro lining, heating at 200-230 DEG C for 6-8 hours, cooling to room temperature, filtering, washing, and drying to obtain the sulfur-doped near-infrared carbonized polymer for detecting mercury ions. And after the reaction kettle is naturally cooled to the room temperature, carrying out continuous dialysis for 24-48 hours by adopting a 1000 Da dialysis bag to remove unreacted impurities, and freeze-drying for 24-48 hours under the conditions that the temperature is-20-30 DEG C and the pressure is-0.1-0.01 Pa to obtain black particles, namely the sulfur-doped near-infrared carbonized polymer for detecting mercury ions. The sulfur-doped near-infrared carbonized polymer for detecting mercury ions has the advantages of strong stability, good selectivity, high sensitivity and high accuracy.

The invention discloses a fluorescent carbon quantum dot, a preparation method thereof and application of the fluorescent carbon quantum dot in atrazine detection, and belongs to the technical field of pesticide residue detection. The carbon quantum dots are obtained by taking citric acid and o-phenylenediamine as raw materials and a mixed solution of ethanol and water as a solvent through the steps of hydrothermal reaction, centrifugation, dialysis, nitrogen blowing, freeze drying and the like. The preparation method provided by the invention has low requirements on equipment, and is simple to operate and low in cost. The fluorescent carbon quantum dots prepared by the method have the characteristics of good fluorescence stability, good water solubility, good selectivity to atrazine, high sensitivity and the like. The detection method shows that the fluorescence enhancement degree of the fluorescent carbon quantum dots and the atrazine concentration are in a linear correlation trend, the detection limit of the detection method is as low as 20ng / L, and the detection result is rapid and accurate. Therefore, the method can be used for rapidly, accurately and sensitively detecting the atrazine in an actual sample and the like.

The invention provides a preparation method and device of quinoxaline compounds, and belongs to the technical field of quinoxaline compound synthesis. The preparation method of the quinoxaline compound comprises the following steps: mixing a substituted 1, 2-dicarbonyl compound and a substituted o-phenylenediamine compound to obtain a mixed raw material; and blowing the mixed raw material into a reaction chamber for reaction to obtain the quinoxaline compound, and finally, carrying out vacuum drying on the quinoxaline compound. High-speed spiral gas is formed in the reaction cavity through high-pressure carrier gas, and the mixed raw materials react in the high-speed spiral gas flow. The preparation process provided by the invention has the advantages of low equipment investment, simple process, high reaction speed, high yield, no need of participation of any solvent and catalyst, environmental friendliness and capability of reducing the production cost.

The invention belongs to the technical field of carbon quantum dots, and discloses a metal-doped carbon quantum dot and a preparation method thereof, the preparation method comprises the following steps: 1) reacting o-phenylenediamine with 2, 5-dihydroxybenzaldehyde to obtain a Schiff base compound; 2) coordinating the Schiff base compound with metal to obtain a metal coordinated Schiff base compound; and 3) dissolving the metal-coordinated Schiff base compound in a solvent, then adding a carboxyl-containing compound, completely dissolving, carrying out heating reaction, after the reaction is finished, carrying out centrifugal separation, reserving supernate, dialyzing, and freeze-drying, so as to obtain the metal-doped carbon quantum dots. The Schiff base metal complex is used as a precursor, nitrogen atoms in Schiff base can form coordinate bonds with metal ions, the stability of the complex is improved through formation of the chemical bonds, the complex can be doped with metal with a stable structure, and it is ensured that the metal is doped to the center of the carbon quantum dot instead of the surface of the carbon quantum dot; therefore, high doping rate and better product performance are obtained.

The invention provides a methcathinone imprinted electrochemical sensor as well as a preparation method and a detection method thereof, and relates to an electrochemical sensor technology. According to the methcathinone imprinted electrochemical sensor, o-phenylenediamine is taken as a polymeric monomer, methcathinone is taken as a template molecule, a methcathinone imprinted membrane is prepared by adopting an electropolymerization mode, and then the methcathinone imprinted membrane is subjected to potential elution in an HCl dilute solution, so that the methcathinone imprinted electrochemical sensor is obtained. The methcathinone imprinted electrochemical sensor capable of detecting methcathinone in a simply treated aqueous solution is prepared, and indirect detection of methcathinone is realized. The sensor is simple to prepare and high in sensitivity, the detection limit can reach 1.0 * 10 <-12 > mol.L <-1 >, the detection range is 1.0 * 10 <-12 >-1.0 * 10 <-4 > mol.L <-1 >, on-site rapid detection of methcathinone can be realized, and a choice is provided for more efficient and rapid inspection and analysis of basic-level public security drugs.

The invention relates to a dual-targeting fluorescent carbon dot for endoplasmic reticulum and lipid droplets and a preparation method and application thereof.The preparation method comprises the steps that o-phenylenediamine and phenylalanine are dissolved in pure water to obtain a first dissolved solution; transferring the first dissolving solution into a poly-tetrafluoroethylene lined reaction kettle, carrying out a first heating reaction to obtain a precipitate, and sequentially carrying out column chromatography purification, rotary evaporation and drying on the precipitate to obtain a target solid; dissolving caffeic acid in dichloromethane, adding CDI, and stirring to obtain a second dissolved solution; and adding the target solid into the second dissolving solution, and carrying out a second heating reaction to obtain the dual-targeting fluorescent carbon dots for the endoplasmic reticulum and the lipid droplets. The dual-targeting fluorescent carbon dots obtained by the preparation method can distinguish endoplasmic reticulum and lipid droplets, and solve the problem of lack of life-sensitive dual-targeting fluorescent carbon dots capable of simultaneously positioning the endoplasmic reticulum and lipid droplets and sensitively monitoring microenvironment changes of the endoplasmic reticulum and lipid droplets.

The invention belongs to the field of preparation of photocatalysts, and particularly relates to a preparation method and application of a composite graphite phase carbon nitride (g-C3N4-g-C3N5-NH) photocatalyst. According to the composite catalyst, 3-amino-1, 2, 4-triazole (3-AT) and melamine (C3H6N6) are taken as material cores, ammonium chloride (NH4Cl) is added, mixed calcination is performed, an electronic structure and interlayer spacing are regulated and controlled, and the photocatalytic performance of the g-C3N4-g-C3N5-NH composite photocatalyst is improved. The catalyst is used for photocatalytic coupling reaction of o-phenylenediamine (OPD) and benzaldehyde (BAD) to generate 2-phenylbenzimidazole (2-BZ) and hydrogen peroxide (H2O2), and has high catalytic activity.

The invention discloses a preparation method of a plastic wood-based filtering material for removing micro-plastics in water, which comprises the following steps: taking natural wood, putting the wood into a treating fluid containing sodium hydroxide and sodium sulfite, treating, air-drying, immersing into water to obtain flexible wood, and collecting the treated fluid for later use; the flexible wood is placed in deionized water, EPTMAC and a sodium hydroxide solution are added, and then heating treatment is conducted to obtain modified wood; extracting lignin from the treated solution, dissolving the lignin and o-phenylenediamine in an acid solution for heating reaction, cooling, centrifuging, dialyzing supernate to obtain a solution containing carbon dots, and drying to obtain carbon dot powder; performing ultrasonic treatment on carbon dot powder, adding the carbon dot powder into absolute ethyl alcohol to obtain a carbon dot ethanol solution, uniformly dropwise adding the carbon dot ethanol solution onto the modified wood, and then drying the modified wood to obtain the plastic wood-based filter material. The plastic wood-based filtering material prepared by the preparation method has good hydrophilicity and plasticity, and can effectively adsorb micro-plastics.

The invention belongs to the technical field of light conversion materials, and provides a carbon dot capable of absorbing ultraviolet light and green light, a light conversion film and a preparation method and application of the light conversion film. The preparation method of the carbon dots capable of absorbing the ultraviolet light and the green light comprises the following steps: mixing o-phenylenediamine, acrylic acid and water, then carrying out hydrothermal reaction, and sequentially carrying out centrifugation, filtration, dialysis and freeze drying on a reaction product, so as to obtain the carbon dots capable of absorbing the ultraviolet light and the green light. A light conversion film prepared by using the carbon dots capable of absorbing the ultraviolet light and the green light as a light conversion agent can be used for plant growth. The light conversion film not only can block and absorb ultraviolet light and convert the ultraviolet light into spectrums absorbed by plants in a blue light area and a red light area, but also can block and absorb green light and convert the green light into spectrums absorbed by the plants in the red light area, so that the photosynthesis of the plants is improved, the germination, growth and development of plant seeds are promoted, and the crop yield is increased.

The invention aims to provide a preparation method and application of a green fluorescent carbon dot for detecting captopril, and belongs to the technical field of fluorescent carbon dots, diphenylamino-4-benzaldehyde and o-phenylenediamine are weighed and dissolved in ethanol, and ultrasonic treatment is performed to obtain a uniformly mixed solution; transferring the solution into a polytetrafluoroethylene reaction kettle, reacting for 2-8 hours at the temperature of 150-220 DEG C, standing and cooling to room temperature after the reaction is stopped, centrifuging to remove insoluble substances, taking supernate, and dialyzing for at least three days in a glass container through a dialysis bag of 500-1000Da to obtain a pure carbon dot aqueous solution; and freeze-drying the carbon dot aqueous solution to obtain a carbon dot solid. The prepared green fluorescent carbon dots can be used for detecting captopril, and are good in selectivity and high in sensitivity. Compared with other carbon dot-based fluorescence sensing methods, the method provided by the invention can be used for directly detecting captopril.

The invention relates to the field of biological detection, in particular to a self-repairing anti-interference film and a preparation method and application thereof. The invention provides a self-repairing anti-interference film. The self-repairing anti-interference film comprises a first electro-polymerization film and a second electro-polymerization film, the first electro-polymerization film is formed by an anti-interference monomer; the anti-interference monomer comprises any one of o-phenylenediamine, p-phenylenediamine and m-phenylenediamine; the second electro-polymerization film is formed by an anti-interference monomer and an interferent comonomer; the anti-interference monomer comprises any one of o-phenylenediamine, p-phenylenediamine and m-phenylenediamine; the interferent comonomer is prepared from acetaminophen. According to the self-repairing anti-interference film provided by the invention, electro-polymerization is completed at one time before leaving a factory. Instrument complexity and process cost are reduced, and no subsequent repair program causes occupation and exclusion to use of a user. In addition, compared with a coating mode of electropolymerization, ideal film thickness can be formed by controlling monomer concentration, electropolymerization parameters and the like, and the process reproducibility is good.

The invention relates to the technical field of nanometer detection, in particular to a preparation method and application of nitrogen-sulfur-doped fluorescent carbon dots for detecting arsenic elements. Comprising the following steps: dissolving thiourea and o-phenylenediamine in formamide, transferring the solution into a polytetrafluoroethylene reaction kettle for reaction, cooling the product to room temperature, centrifuging, collecting supernate, filtering by using an organic filter membrane, dialyzing by using a dialysis bag, filtering by using a water-phase filter membrane, putting into a rotary evaporator for rotary evaporation concentration, and freeze-drying the product to obtain the o-phenylenediamine. The nitrogen and sulfur doped fluorescent carbon dot powder capable of being used for detecting the arsenic element is obtained; the fluorescent carbon dots are simple to synthesize, quick in response and high in sensitivity, arsenic can be qualitatively and quantitatively detected without chemically modifying the detection probe, and the fluorescent carbon dots have a good application prospect.

The invention belongs to the technical field of fluorescent probes, and particularly relates to a ratio fluorescent probe based on red / blue double-color carbon dots as well as a preparation method and application of the ratio fluorescent probe. The ratio fluorescent probe based on the red-blue double-color carbon dots is obtained by mixing a blue fluorescent carbon dot solution and a red fluorescent carbon dot solution according to the volume ratio of 6: 1, and the concentrations of the blue fluorescent carbon dot solution and the red fluorescent carbon dot solution are both 0.5 mg / mL; the blue fluorescent carbon dot solution is prepared by the following steps: adding deionized water into a mixed solution of citric acid and ethidene diamine, carrying out ultrasonic treatment, and then carrying out reaction centrifugation, filtration and dialysis to obtain blue fluorescent carbon dots; the red fluorescent carbon dot solution is prepared by adding deionized water and concentrated sulfuric acid into o-phenylenediamine, L-cysteine and boric acid, carrying out ultrasonic treatment, reacting, centrifuging, filtering, dialyzing and freeze-drying. The ratio fluorescent probe based on the red / blue double-color carbon dots can accurately detect the concentration of quercetin in the concentration range of 0-30.0 [mu] mol / L.

The present invention belongs to the field of nanomaterials, and relates to kilogram-level full-color high-efficiency solid-state fluorescent carbon dots and their preparation methods. The method for synthesizing full-color solid-state fluorescent carbon dots comprises the following steps: o-phenylenediamine and 1,2,4,5-pyromellitic anhydride are dissolved in an ethanol aqueous solution according to a molar ratio of 1: (1-11); the resulting solution is then subjected to microwave heating treatment, the microwave heating power is 500-1000W, and the microwave heating time is 5-25min to obtain a carbon dot crude liquid; after the obtained carbon dot crude liquid is naturally volatilized, dimethyl sulfoxide is added thereto to fully dissolve, and centrifuge; the supernatant is taken, water is added, and filtered; the precipitate is taken, dried, and solid-state fluorescent carbon dot powder is obtained; by adjusting the molar ratio of o-phenylenediamine and 1,2,4,5-pyromellitic anhydride, and the microwave heating time, blue solid-state fluorescent carbon dot powder, green solid-state fluorescent carbon dot powder, yellow solid-state fluorescent carbon dot powder, and red solid-state fluorescent carbon dot powder are obtained respectively. The method of the present invention obtains kilogram-level full-color solid-state fluorescent carbon dots with a quantum yield of up to 55.3-27.9% for the first time.

The invention discloses a synthesis method of 5-(1H-pyrrole-1-yl)-2-mercaptobenzimidazole, which comprises the following steps: carrying out cyclization condensation reaction on o-phenylenediamine and thiourea in an acidic environment provided by hydrochloric acid to obtain 2-mercaptobenzimidazole; the preparation method comprises the following steps: carrying out nitration reaction on 2-mercaptobenzimidazole, sulfuric acid and nitric acid to obtain 2-mercapto-5-nitrobenzimidazole; the preparation method comprises the following steps: carrying out nitro reduction reaction on 2-sulfydryl-5-nitrobenzimidazole and hydrogen under the catalysis of nickel to obtain 5-amino-2-sulfydryl benzimidazole; the preparation method comprises the following steps: carrying out an N-alkylation reaction on 5-amino-2-mercapto benzimidazole and tetrahydro-2, 5-dimethoxyfuran under the catalysis of copper chloride to generate 5-(1H-pyrrole-1-yl)-2-mercapto benzimidazole, and carrying out a reaction on the 5-amino-2-mercapto benzimidazole and tetrahydro-2, 5-dimethoxyfuran to generate the 5-(1H-pyrrole-1-yl)-2-mercapto benzimidazole. The method disclosed by the invention has the outstanding advantages of mild and easily-controlled conditions, simple and convenient post-treatment, high yield, economy, environmental protection, suitability for large-scale production and the like, conforms to the green chemical development concept, and has important application value.

The invention belongs to the technical field of materials, and particularly relates to a preparation method of a PoPD-MnO2 positive electrode material and application of the PoPD-MnO2 positive electrode material in an aqueous zinc ion battery. The preparation method comprises the following steps: dissolving potassium permanganate and o-phenylenediamine in deionized water, stirring at room temperature, transferring the fully mixed solution into a hydrothermal reaction kettle, carrying out hydrothermal reaction, cooling to room temperature, carrying out centrifugal cleaning, and carrying out vacuum drying to obtain the PoPD-MnO2 positive electrode material. According to the invention, ammonium ions are inserted into the electrode material, and the ammonium ions are inserted into MnO2, so that the interlayer spacing is enlarged, the active sites are increased, ion diffusion and charge transfer are effectively promoted, the conductivity of the material is improved, the internal resistance of the material is reduced, and the structural flexibility and stability of the material are maintained; therefore, the overall performance of the water-based zinc ion battery is improved.

The invention belongs to the technical field of food safety detection, and particularly relates to folic acid-o-phenylenediamine co-doped carbon quantum dots, an Escherichia coli O157: H7 detection probe, a detection reagent and application. The folic acid-o-phenylenediamine co-doped carbon quantum dots are prepared by heating folic acid and o-phenylenediamine to react. When the detection probe or the detection reagent based on the folic acid-o-phenylenediamine co-doped carbon quantum dots is used for detecting Escherichia coli O157: H7, the detection limit is low, the specificity is excellent, and the practicability is high.

A water-soluble red-light carbon dot and its preparation method and application, the method comprising the following steps: adding dicyandiamide and o-phenylenediamine to deionized water containing sulfuric acid and stirring for 20-30 minutes to form a suspension; placing the suspension in an autoclave and heating the reaction at 100-200°C for 6-12 hours to form a dark blue carbon dot solution; after cooling the dark blue carbon dot solution to room temperature, filtering it with a water-based microporous membrane and dialysis bag, respectively, to remove impurities and obtain pure water-soluble red-light carbon dots. This method has the advantages of simple process, easy operation, low cost, and no pollution, and can be used for industrial production; the prepared water-soluble red-light carbon dots are small in size, have good monodispersity, a graphene-like structure, and water solubility, and can significantly improve the absorption and transport of potassium and sodium ions by the roots of field crops under drought stress and salt stress, thereby increasing the photosynthetic rate and stomatal conductance of the crops and alleviating drought and salt stress in field crops.

The invention belongs to the technical field of food safety detection, and particularly relates to a histamine molecular imprinting electrochemical sensor based on a carbon-based nano composite material and a preparation method and application of the histamine molecular imprinting electrochemical sensor. Aiming at the problems of poor selectivity, high detection limit or complicated operation and the like of the existing histamine detection technology, a Co3O4 / N-HCS (cobaltosic oxide / nitrogen-doped hollow carbon sphere) composite material is prepared by adopting a solvothermal method, o-phenylenediamine (o-PD) is taken as a functional monomer, a molecularly imprinted polymer membrane (MIP) is constructed on the surface of a glassy carbon electrode (GCE) by adopting an electropolymerization method, and the histamine detection electrode is prepared. The MIP / Co3O4 / N-HCS / GCE sensor with specific recognition sites on histamine is formed. The sensor has high selectivity, high sensitivity and good stability, is simple and convenient to operate and rapid in detection, can efficiently realize accurate detection of histamine in food, and is suitable for safety monitoring scenes of various foods such as fermented food, aquatic products and dairy products.

The application discloses a 4-(1H-benzo[d]imidazol-2-yl)-N,N-bis(2-chloroethyl) aniline derivative and a preparation method and application thereof, and belongs to the technical field of biological medicines. A structural formula is shown in the following formula I: wherein the R substituent is selected from any one of hydrogen, halogen, a carboxyl group, a cyano group, a nitro group and an alkoxy group. The application takes 4-position substituted o-phenylenediamine and 4-[bis(beta-chloroethyl)amino]benzaldehyde as raw materials, and obtains a target molecule through a condensation reaction. At present, similar nitrogen mustard or benzimidazole drug synthesis needs multiple steps of reaction, and separation and purification is complicated. The application has only one step of synthesis, a simple separation method and low requirement on equipment. In vitro anti-tumor experiments show that the target compounds synthesized by the application all have obvious anti-tumor effects, exhibit strong pharmacological activities in inhibiting tumor cell proliferation, and have wide research and development value in the field of anti-tumor drugs.

The invention belongs to the technical field of electrochemical analysis and detection, and particularly relates to an electrochemical immunosensor for detecting adiponectin as well as a preparation method and application of the electrochemical immunosensor. The preparation method comprises the following steps: (1) connecting a screen-printed electrode SPCE to an electrochemical workstation by using a 0.01 M PBS (Phosphate Buffer Solution) with the pH value of 7.4, performing-0.2 V-1. 2V cyclic voltammetry scanning, and activating for 10 circles under 50mV / S; and (2) by taking 0.01-1M H2SO4 as a base solution, connecting the screen-printed electrode SPCE to an electrochemical workstation, carrying out electro-polymerization on poly-o-phenylenediamine in a 50-100mM o-phenylenediamine and NH2-UiO-66 solution to form a poly-o-phenylenediamine and NH2-UiO-66 composite modified electrode, washing with deionized water, and drying at room temperature. (3) soaking the modified electrode in a glutaraldehyde solution for 10-70 minutes, cleaning with deionized water, drying at room temperature, adding 10-100 [mu] L of ADPN antibody solution and 100-600 [mu] g / mL of ADPN antibody solution, and incubating; and (4) washing with ionized water, drying at room temperature, preventing non-specific binding sites at 37 DEG C for 0.5-1.5 hours by using 3-10 microliters of 0.8-1.2 wt% casein solution, and washing the screen-printed electrode again to obtain the electrochemical immunosensor.

The invention discloses a double-emission carbon dot hydrogel film as well as a preparation method and application thereof, and relates to the technical field of analysis detection and functional materials. The double-emission carbon dot hydrogel film takes polyvinyl alcohol hydrogel as a carrier, and double-emission carbon dots (O-CDs) synthesized by taking L-glutamic acid and o-phenylenediamine as precursors are uniformly dispersed in the polyvinyl alcohol hydrogel. The O-CDs have double emission peaks of 400 nm and 530 nm under the excitation of 375 nm. The dual-emission carbon dot hydrogel film is applied to portable visual detection of Cu < 2 + > and Ag < + >, and the portable visual detection is dual-mode measurement based on RGB and comprises fluorescence colorimetric measurement in a solution and visual detection of the film assisted by a smart phone. According to the dual-mode strategy, precise instruments are not needed, rapid, reliable and synchronous detection of Cu < 2 + > and Ag < + > can be achieved within 10 min, the limitation of traditional single-mode detection is broken through, and a stable platform is provided for on-site metal ion monitoring.

The invention discloses bio-based polyamide with low water absorption and a preparation method thereof, and belongs to the technical field of polyamide preparation. The bio-based polyamide with the low water absorption rate is prepared from the following components in parts by weight: 30 to 50 parts of pentamethylene diamine, 15 to 30 parts of sebacic acid, 5 to 12 parts of citric acid, 5 to 10 parts of hexamethylenediamine, 2 to 5 parts of a hydrophobic auxiliary agent and 1 to 5 parts of a modified high-temperature-resistant additive, the hydrophobic additive is prepared from acetoxybenzoic acid, nonadecanoic acid, m-dimethoxybenzene, trifluoroacetic anhydride and methyl tert-butyl ether as raw materials, and the modified high-temperature-resistant additive is prepared from o-phenylenediamine, chloroacetic acid, vanillin, benzoyl bromide and thiourea as raw materials. The bio-based polyamide prepared from the substances has low water absorption and excellent thermal stability.

The application belongs to the technical field of fluorescent nanomaterials, and provides nitrogen-doped carbon dots, a preparation method thereof and application of the nitrogen-doped carbon dots in colorimetric / fluorescent dual-mode visual detection of pH and histamine, to solve the problem that regular colorimetric signals of current carbon dots are difficult to achieve, which limits the application of the carbon dots in biomedicine and photoelectric devices. The nitrogen-doped fluorescent carbon dots are synthesized by one-step hydrothermal reaction of o-phenylenediamine and 2,6-pyridinedicarboxylic acid, water and hydrochloric acid. The preparation method is simple and fast, the synthesized carbon dots have good water solubility and biocompatibility, stable optical properties, high fluorescence quantum yield, can quickly and visually detect intracellular pH and histamine in food spoilage process, have excellent selectivity and anti-interference ability, fast response speed, high sensitivity, and can realize on-site and instant detection by means of a smart phone and a test strip.