Method for preparing two-waveband single-photon and two-photon fluorescent carbon quantum dots and application

Abstract

The invention provides a method for preparing two-waveband single-photon and two-photon fluorescent carbon quantum dots and application, and the method comprises the following steps: preparing an o-phenylenediamine solution or a mixed solution of the o-phenylenediamine and an acid; putting the o-phenylenediamine solution or the o-phenylenediamine mixed solution into a reaction kettle, heating, reacting to obtain a product, and carrying out centrifugal separation on the product to obtain the two-waveband fluorescent carbon quantum dots with single-photon and two-photon fluorescence emission characteristics at the same time. The preparation method disclosed by the invention is simple, economical and easy to operate, can be used for preparing two-waveband long-waveband fluorescence luminous carbon quantum dots at one time, has the characteristics of long-waveband single-photon and two-photon fluorescence excitation and emission, and has a good application prospect in the biomedical fieldsof fluorescence labeling of biological cells and tissues, living body imaging and the like.

Description

technical field

[0001] The invention relates to the field of fluorescent material synthesis, in particular to a method and application for preparing two-band single-photon and two-photon fluorescent carbon quantum dots. Background technique

[0002] Fluorescent nanoparticles have good application prospects in the important fields of biomedicine, LED luminescence, and metal ion detection. However, traditional organic fluorescent dyes have poor stability and are prone to photocatalytic degradation. Semiconductor quantum dot materials have good photostability and high fluorescence yield. However, they have poor solubility in aqueous solution and contain heavy metal elements such as Se, Te, and Cd, which will cause serious damage to human health and environmental pollution. This severely limits the application range of semiconductor quantum dot materials.

[0003] As a new type of fluorescent nanoparticle, carbon quantum dots have a series of excellent properties such as tunabl...

Examples

Embodiment 1

[0036] This embodiment provides a method and application for preparing two-band single-photon and two-photon fluorescent carbon quantum dots based on o-phenylenediamine, which is applied to LED light emission, as follows.

[0037] Take two 100mL beakers and wash them in ultrapure water for 30 minutes. After cleaning, dry them for later use.

[0038] Use a 5mL pipette gun to absorb 15mL of ultrapure water and add it to one of the beakers. Use an electronic balance to weigh 200 mg of o-phenylenediamine and add it to the beaker. After ultrasonication for 30 minutes, a clear and transparent solution is obtained.

[0039] Then use a 5mL pipette gun to draw the obtained transparent solution, add it into a Teflon reactor with a capacity of 50mL, and heat it at a temperature of 220°C, and heat it at a constant temperature for 6 hours. min -1 Centrifuge for 10 min.

[0040] Then absorb the supernatant after centrifugation, put it into a dialysis bag with a molecular weight cut off of...

Embodiment 2

[0045] This embodiment provides a method and application for preparing two-band single-photon and two-photon fluorescent carbon quantum dots based on o-phenylenediamine, and the application is for two-photon fluorescent labeling of Escherichia coli under the condition of pH=1, as follows.

[0046] Take two 100mL beakers, wash them in ultrapure water for 30 minutes, and dry them for later use.

[0047] Use a 5mL pipette gun to draw 15mL of ultrapure water into one of the beakers, weigh 100mg of o-phenylenediamine with an electronic balance, add it to the beaker and ultrasonically dissolve it for 30 minutes, and obtain a clear and transparent o-phenylenediamine solution, add it to the o-phenylenediamine solution 0.5mL of phosphoric acid was prepared into a mixed solution of o-phenylenediamine.

[0048] Use a 5mL pipette gun to draw 15mL o-phenylenediamine mixed solution and add it into a Teflon polytetrafluoroethylene reactor with a capacity of 50mL and heat it at a temperature ...

Embodiment 3

[0054] This embodiment provides a method and application for preparing two-band single-photon and two-photon fluorescent carbon quantum dots based on o-phenylenediamine, which is applied to the metal ion Fe under the condition of pH=0. 3+ and Au 3+ Selective fluorescence quenching, as follows.

[0055] Take two 100mL beakers and wash them in ultrapure water for 30 minutes. After cleaning, dry them for later use.

[0056] Use a 5mL pipette gun to draw 15mL of ultrapure water into one of the beakers, weigh 150mg of o-phenylenediamine with an electronic balance, add it to the beaker and ultrasonically dissolve it for 30min, and obtain a clear and transparent solution.

[0057] Add 1.35 g of sodium sulfate to the solution to prepare a mixed solution of o-phenylenediamine. Use a 5mL pipette gun to draw the above solution into a Teflon polytetrafluoroethylene reactor with a capacity of 50mL and heat at a heating temperature of 180°C for 4 hours at a constant temperature. After th...

technical field

[0001] The invention relates to the field of fluorescent material synthesis, in particular to a method and application for preparing two-band single-photon and two-photon fluorescent carbon quantum dots. Background technique

[0002] Fluorescent nanoparticles have good application prospects in the important fields of biomedicine, LED luminescence, and metal ion detection. However, traditional organic fluorescent dyes have poor stability and are prone to photocatalytic degradation. Semiconductor quantum dot materials have good photostability and high fluorescence yield. However, they have poor solubility in aqueous solution and contain heavy metal elements such as Se, Te, and Cd, which will cause serious damage to human health and environmental pollution. This severely limits the application range of semiconductor quantum dot materials.

[0003] As a new type of fluorescent nanoparticle, carbon quantum dots have a series of excellent properties such as tunabl...

Examples

Embodiment 1

[0036] This embodiment provides a method and application for preparing two-band single-photon and two-photon fluorescent carbon quantum dots based on o-phenylenediamine, which is applied to LED light emission, as follows.

[0037] Take two 100mL beakers and wash them in ultrapure water for 30 minutes. After cleaning, dry them for later use.

[0038] Use a 5mL pipette gun to absorb 15mL of ultrapure water and add it to one of the beakers. Use an electronic balance to weigh 200 mg of o-phenylenediamine and add it to the beaker. After ultrasonication for 30 minutes, a clear and transparent solution is obtained.

[0039] Then use a 5mL pipette gun to draw the obtained transparent solution, add it into a Teflon reactor with a capacity of 50mL, and heat it at a temperature of 220°C, and heat it at a constant temperature for 6 hours. min -1 Centrifuge for 10 min.

[0040] Then absorb the supernatant after centrifugation, put it into a dialysis bag with a molecular weight cut off of...

Embodiment 2

[0045] This embodiment provides a method and application for preparing two-band single-photon and two-photon fluorescent carbon quantum dots based on o-phenylenediamine, and the application is for two-photon fluorescent labeling of Escherichia coli under the condition of pH=1, as follows.

[0046] Take two 100mL beakers, wash them in ultrapure water for 30 minutes, and dry them for later use.

[0047] Use a 5mL pipette gun to draw 15mL of ultrapure water into one of the beakers, weigh 100mg of o-phenylenediamine with an electronic balance, add it to the beaker and ultrasonically dissolve it for 30 minutes, and obtain a clear and transparent o-phenylenediamine solution, add it to the o-phenylenediamine solution 0.5mL of phosphoric acid was prepared into a mixed solution of o-phenylenediamine.

[0048] Use a 5mL pipette gun to draw 15mL o-phenylenediamine mixed solution and add it into a Teflon polytetrafluoroethylene reactor with a capacity of 50mL and heat it at a temperature ...

Embodiment 3

[0054] This embodiment provides a method and application for preparing two-band single-photon and two-photon fluorescent carbon quantum dots based on o-phenylenediamine, which is applied to the metal ion Fe under the condition of pH=0. 3+ and Au 3+ Selective fluorescence quenching, as follows.

[0055] Take two 100mL beakers and wash them in ultrapure water for 30 minutes. After cleaning, dry them for later use.

[0056] Use a 5mL pipette gun to draw 15mL of ultrapure water into one of the beakers, weigh 150mg of o-phenylenediamine with an electronic balance, add it to the beaker and ultrasonically dissolve it for 30min, and obtain a clear and transparent solution.

[0057] Add 1.35 g of sodium sulfate to the solution to prepare a mixed solution of o-phenylenediamine. Use a 5mL pipette gun to draw the above solution into a Teflon polytetrafluoroethylene reactor with a capacity of 50mL and heat at a heating temperature of 180°C for 4 hours at a constant temperature. After th...

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