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Fluorescence sensor for detecting uric acid and preparation method thereof

A fluorescent sensor and uric acid technology, applied in the direction of fluorescence/phosphorescence, instruments, measuring devices, etc., can solve the problems of complex material preparation and complicated detection process, and achieve the effect of rapid detection method and overcoming the large interference of coexisting substances

Active Publication Date: 2018-06-12
FUZHOU UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0003] There are few reports on the construction of biosensors based on three-dimensional metal-organic frameworks for the detection of uric acid, such as the use of UIO-66-NO 2 @XC-72 composite material for the detection of uric acid (RSC Adv., 2017, 7, 5628-5635) and the use of CD-MONT-2′ to construct a fluorescence-enhanced fluorescent sensor for the detection of uric acid (J. Mater. Chem. C, 2017,5, 601-606), but these works have the disadvantages of complicated material preparation and complicated detection process

Method used

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  • Fluorescence sensor for detecting uric acid and preparation method thereof
  • Fluorescence sensor for detecting uric acid and preparation method thereof
  • Fluorescence sensor for detecting uric acid and preparation method thereof

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preparation example Construction

[0046] A method for preparing a fluorescent sensor for detecting uric acid, comprising the following steps:

[0047] 1) Prepare uric acid solutions with different concentration units;

[0048] 2) The two-dimensional metal-organic framework material NH with fluorescent properties 2 -BDC-MOF nanosheets were dispersed in ultrapure water and ultrasonicated for 30 min, centrifuged to take the supernatant for later use, and the NH in the supernatant 2 -The concentration of BDC-MOF nanosheets is 200 μg / mL;

[0049] 3) Dissolve uricase in tris(hydroxymethyl)aminomethane-hydrochloride buffer solution to prepare uricase solution, and store it in the dark at 4°C for later use;

[0050] 4) Dissolve o-phenylenediamine in tris(hydroxymethyl)aminomethane-hydrochloride buffer solution to prepare o-phenylenediamine solution, and store it in the dark at 4°C for later use;

[0051] 5) Take the 200μg / mL NH prepared in step 2) respectively 2 -50 μL of BDC-MOF nanosheet solution, step 3) prepar...

Embodiment 1

[0073] Example 1 (the method of the present invention detects uric acid)

[0074] 1. First synthesize NH 2 -BDC-MOF nanosheets, the process is as follows:

[0075] 1) Dissolve 1.5 mg of o-aminoterephthalic acid in 3 mL of a mixed solvent of N,N-dimethylformamide and acetonitrile with a volume ratio of 2:1;

[0076] 2) Dissolve 3.0 mg of copper nitrate trihydrate in 3 mL of a mixed solvent of N,N-dimethylformamide and acetonitrile with a volume ratio of 1:2;

[0077] 3) Prepare 2 mL of a mixed solvent of N,N-dimethylformamide and acetonitrile with a volume ratio of 1:1 as a linker;

[0078] 4) The reaction was carried out in a test tube with a diameter of 1.5 cm and 25 mL. The o-aminoterephthalic acid solution was first added to the bottom of the test tube, then 2 mL of linking agent was added, and finally the solution of copper nitrate dissolved in the upper layer was added;

[0079] 5) In a 40°C water bath, heat the o-aminoterephthalic acid layer and react for 24 hours;

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Abstract

The invention belongs to the field of metal organic framework materials used for analyzing and detecting disease markers and specifically relates to a fluorescence sensor for detecting uric acid and apreparation method thereof. Different concentrations of uric acid solutions with the same volume are vibrated and mixed with an NH2-BDC-MOF nanosheet solution, an uricase solution and an o-phenylenediamine solution, the uric acid solutions are stood under 37 DEG C for 30 minutes, and fluorescence signals are detected to construct a fluorescence signal-concentration work curve; then fluorescence signals in a sample are detected and substituted into the work curve to achieve the purpose of quantitatively analyzing uric acid in the sample. The method has the advantages of quickness, flexibilityand high efficiency.

Description

technical field [0001] The invention belongs to the field of analysis and detection of metal organic framework materials used for disease markers, and in particular relates to a fluorescent sensor for detecting uric acid and a preparation method thereof. Background technique [0002] The biological molecule uric acid (2,4,6-trihydroxypurine, referred to as UA) is the final product of purine metabolism in the human body and plays an important role in the metabolism of the human body. Abnormal uric acid is often used as a sign of gout, kidney disease, hyperuricemia and other diseases. Changes in uric acid content in body fluids can fully reflect the status of metabolism, immunity and other functions in the human body. Its molecular formula is C 15 h 14 N 4 o 3 , insoluble in water, insoluble in acid, and easily soluble in alkaline conditions, which greatly limits the detection of the substance. Commonly used detection methods include liquid chromatography, fluorescence, an...

Claims

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Application Information

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IPC IPC(8): G01N21/64B82Y30/00
CPCB82Y30/00G01N21/6428
Inventor 邱彬胡水生郭隆华林振宇陈国南
Owner FUZHOU UNIV
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