Method of improving salt resistance of bluegrass by using exogenous proline
A technology of exogenous proline and proline, which is applied in the field of improving the salt resistance of bluegrass, can solve the problems of general salt resistance effect, long treatment cycle, complicated process, etc. simple effect
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Embodiment 1
[0021] Embodiment 1 Concentration selection test
[0022] Adding 100 mmol / L NaCl to stress Poa annua, the growth of Poa annua was severely inhibited. After 10 days of stress treatment, the aboveground biomass and belowground biomass of Poa annua decreased by 46.0% and 44.8%, respectively, compared with those cultured in normal nutrient solution. Randomly divided into 9 groups.
[0023] Method: Proline solutions with concentrations of 0.02 mmol / L, 0.05 mmol / L, 0.1 mmol / L, 0.20 mmol / L, 0.50 mmol / L, 0.80 mmol / L, 1.60 mmol / L and 3.20 mmol / L were prepared respectively (Pro) solution, pour the solution into the sprayer for foliar application; spray 2 times a day for 3 days. The result is as Figure 1-Figure 4 .
[0024] Salt stress can inhibit the growth of most plants to varying degrees. The results of this experiment also showed that 100 mmol / L NaCl stress treatment significantly inhibited the growth of Poa annua. Adopt the method of the present invention, from Figure 1-F...
Embodiment 2
[0027] Embodiment 2 The selection of spraying material
[0028] Adding 100 mmol / L NaCl to stress Poa annua, the growth of Poa annua was severely inhibited. After 10 days of stress treatment, the aboveground biomass and belowground biomass of Poa annua decreased by 46.0% and 44.8%, respectively, compared with those cultured in normal nutrient solution. Randomly divided into 4 groups.
[0029] Method: Proline solution (Pro) solution, 0.50 mmol / L salicylic acid (SA) solution, 10 mmol / L CaCl 2 Solution, 10μmol / L gibberellin (GA 3 ) solution and injected into the sprayer for foliar spraying; spraying twice a day for 3 days. The result is as Figure 5-Figure 6 .
[0030] From Figure 5 and Figure 6 Visible, SA, GA 3 , CaCl 2 , proline can alleviate the effect of salt stress, SA will be transformed rapidly in plants, and this substance cannot be conducted in the phloem, so it cannot be effectively used; GA 3 The price is relatively high, and it is easy to decompose under ...
Embodiment 3
[0031] Example 3 A method of improving the salt resistance of bluegrass by using proline
[0032] (1) The method is as follows:
[0033] 1) Prepare a proline solution with a concentration of 0.2 mM, inject the solution into the sprayer, and spray on the leaves;
[0034] 2) Select the bluegrass seedling stage for spraying, and the selected date is between May and June;
[0035] 3) Choose the weather without rain and spray for 3 days;
[0036] 4) Spray twice a day, once before 7:00 am and 18:00 pm;
[0037] 5) The amount of spraying is based on the fact that the leaves are wet but not dripping.
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