Dendrobium officinale tissue culture seedling method

A technology for Dendrobium officinale and tissue culture seedlings is applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of low survival rate, difficult survival, artificial seedling raising and the like, and achieves the effect of improving the survival rate

Inactive Publication Date: 2013-09-11
贵州康旅食斛开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The tender stems of a few varieties such as Dendrobium candidum are twisted into spiral or spring-shaped and dried in the sun. The commercial product is called Dendrobium earrings. Howe

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0010] Dendrobium candidum plantation in Bingshu Town, Congjiang County.

[0011] Put it into the container according to the following mass ratio, then boil it, pour the culture medium into the tissue culture bottle through the filling machine, put it into the sterilizer for sterilization and sterilization, the temperature is 100°C, the pressure is 0.2kPa, and it is sterilized The time is 20 minutes, then take it out and let it cool naturally.

[0012] 1000 grams of peeled bananas are stirred into a paste, 200 grams of white sugar, 95 milliliters of ferrous sulfate, the concentration of ferrous sulfate is 70%, 25 grams of ammonium nitrate, 35 grams of potassium nitrate, 8 grams of magnesium sulfate, 10 milliliters of glycine, the concentration of glycine 98%, Vitamin B 1 2 ml, Vitamin B 1 Concentration of 99%, vitamin B 2 2 ml each, Vitamin B 2 Concentration of 99%, sugar amino acid 20ml, sugar amino acid concentration 98%, naphthaleneacetic acid 20-30ml, naphthaleneacetic...

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Abstract

The invention discloses a dendrobium officinale tissue culture seedling method which comprises the following operating steps of: manufacturing a culture medium of dendrobium officinale tissue culture seedlings, filling the components in a container according to the following weight ratio: 1000-1500g of banana stirred into the paste, 200-300g of white sugar, 90-105ml of ferrous sulfate, 25-33g of ammonium nitrate, 35-40g of potassium nitrate, 5-8g of magnesium sulfate, 8-12ml of glycine, 1-3ml of vitamin B1, 1-3ml of vitamin B2, 20-30ml of sugar amino acid, 20-30ml of naphthylacetic acid and 1000ml of running water, boiling, filling the culture medium into a tissue culture bottle through a filling machine, sterilizing and disinfecting; seeding on the culture medium by using pollen of mature dendrobium officinale seeds, and growing mother seedlings within three months. The cultured dendrobium officinale tissue culture seedlings are healthy and strong and normal in leaf color, 4-5 seedlings are grown, the survival rate is high, and the survival rate can be over 95 percent.

Description

technical field [0001] The invention relates to a method for tissue culture seedlings of dendrobium officinale. Background technique [0002] Dendrobium candidum, also known as black knot grass, belongs to the Dendrobium genus of Orchidaceae, is a perennial epiphytic herb of Orchidaceae, and is a precious wild medicinal plant. Born on the semi-shady rocks in the mountains at an altitude of 1600 meters, it likes a warm and humid climate and a semi-shady and semi-yang environment. It is not cold-resistant, and generally can withstand a low temperature of -5°C. Dendrobium can be divided into dozens of species such as yellow grass, nobile, and horsewhip. Dendrobium candidum and Dendrobium nobile are the best products of dendrobium. Dendrobium candidum is listed as "the first of the nine Chinese immortal grasses". A tribute to the royal family, Dendrobium officinale got its name because of its iron-green skin. Dendrobium candidum has unique medicinal value, and its stem is used...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 叶军剑章琴苍金秦满金奔奔
Owner 贵州康旅食斛开发有限公司
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