Preparation method of cottonseed polypeptide

The technology of cottonseed and cottonseed cake is applied in the field of preparation of cottonseed polypeptide, which can solve the problems of waste discharge polluting the environment and low added value, and achieve the effects of low molecular weight, improved utilization rate and good product stability.

Inactive Publication Date: 2013-09-11
WUHAN POLYTECHNIC UNIVERSITY
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AI Technical Summary

Problems solved by technology

At present, there are dozens of domestic cottonseed oil manufacturers, and a large amount of defatted cottonseed mea...

Method used

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Embodiment

[0027] A method for preparing cottonseed polypeptide, the method comprising the steps of:

[0028] a. Processing of raw materials: crush the degreased cottonseed cake with a pulverizer and pass through a 40-mesh sieve to obtain the crushed cottonseed cake, then mix the crushed cottonseed cake and water evenly at a weight ratio of 1:4 to obtain the cottonseed cake suspension.

[0029] b. Homogenization of the suspension: the cottonseed cake suspension is subjected to colloidal grinding and then homogenized by a high-pressure homogenizer to obtain a cottonseed cake mixture, and the homogenization pressure is 30Mpa.

[0030] c, heat preservation enzymolysis: add the mixed enzyme solution of AlcaLase enzyme and papain to the cottonseed cake mixed solution,

[0031] The volume ratio of AlcaLase enzyme and papain in the mixed enzyme solution is AlcaLase enzyme: papain = 1:1. The amount of mixed enzyme solution added is 0.05% of the mass of the cottonseed cake mixed solution. The v...

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Abstract

The invention provides a preparation method of a cottonseed polypeptide. The method includes the steps of: a. raw material treatment: crushing a low-temperature cold-pressed degreased cottonseed cake, and mixing the crushed cottonseed cake with water uniformly to obtain a cottonseed cake suspension; b. homogenizing of the suspension: homogenizing the cottonseed cake suspension to obtain a cottonseed cake mixed solution; c. heat preservation enzymolysis: adding a mixed enzyme solution of AlcaLase enzyme and papain, carrying out enzymolysis at 50-60DEG C and under a pH value of 7.0-8.5 for 6-8h, thus obtaining an enzymatic hydrolysate of the cottonseed cake; d. high temperature sterilization: rapidly raising the temperature of the enzymatic hydrolysate of the cottonseed cake to 90-95DEG C; e. liquid-solid separation: subjecting the cottonseed cake's enzymatic hydrolysate that undergoes high temperature enzyme deactivation to liquid-solid separation, then removing insoluble solids so as to obtain a cottonseed polypeptide solution; f. concentration: concentrating the cottonseed polypeptide solution to obtain a concentrated cottonseed polypeptide solution; and g. drying: drying the concentrated cottonseed polypeptide solution to obtain cottonseed polypeptide powder.

Description

technical field [0001] The invention belongs to the field of food processing, in particular to a preparation method of cottonseed polypeptide. Background technique [0002] Cottonseed protein content is generally 14% to 17%, up to 29.7%, and its potency is similar to animal protein, so it is a good protein. Cottonseed protein is rich in amino acids, and the content of 18 kinds of amino acids is reasonable, which is close to the standards stipulated by the Food and Agriculture Organization of the United Nations (FAO) and the World Health Organization (WHO). Among them, there are 8 kinds of essential amino acids, and the contents of arginine and glutamic acid are very high. Cottonseed protein is composed of four proteins, which are albumin, globulin, prolamin and glutenin, accounting for 6.81%, 17.57%, 5.33% and 70.11% of the total cottonseed protein, respectively. [0003] my country's cottonseed resources are very rich, but the depth of current development and utilization i...

Claims

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Application Information

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IPC IPC(8): A23J1/14A23J3/34
Inventor 何东平刘良忠胡传荣张世宏姚理
Owner WUHAN POLYTECHNIC UNIVERSITY
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