Application of bacillus coagulans serving as biological preservative in preservation and fresh keeping of pseudosciaena crocea
A technology of Bacillus coagulans and biological preservatives, applied in the field of antiseptic and fresh preservation of aquatic products, can solve the problems of affecting the flavor characteristics of aquatic products, affecting the quality of aquatic products, reducing nutritional value, etc. The effect of freshness
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Embodiment 1
[0024] (1) Cultivation of Bacillus coagulans fermentation broth: First, pick activated Bacillus coagulans (preservation number: CCTCC NO: M 2013193) from the nutrient agar slant medium, inoculate it in the nutrient broth seed medium, 37°C Cultivate on a shaking table for 12 hours, inoculate the seed liquid in the nutrient broth medium at an inoculation amount of 6%, and culture at 37°C for 24 hours to obtain a fermentation liquid. The number of Bacillus coagulans in the fermentation broth was determined to be about 1×10 5 cfu / mL.
[0025] (2) Preparation of Bacillus coagulans suspension: centrifuge the obtained fermentation broth at 4°C, 8000rpm / min for 15min; resuspend the obtained sludge with 0.65% physiological saline.
[0026] (3) Coating treatment: remove the scales, viscera, head and tail of the large yellow croaker, wash the slices, soak in the preservative in step (1) for 5 minutes, take it out and drain for 1 minute, put it on an iron rack and place it Let it dry na...
Embodiment 2
[0029] (1) Cultivation of Bacillus coagulans fermentation broth: First, pick activated Bacillus coagulans (preservation number: CCTCC NO: M 2013193) from the nutrient agar slant medium, inoculate it in the nutrient broth seed medium, 37°C Cultivate on a shaking table for 12 hours, inoculate the seed liquid in the nutrient broth medium at an inoculation amount of 6%, and culture at 37°C for 24 hours to obtain a fermentation liquid. The number of Bacillus coagulans in the fermentation broth was determined to be about 1×10 5 cfu / mL.
[0030] (2) Preparation of Bacillus coagulans suspension: centrifuge the obtained fermentation broth at 4°C, 8000rpm / min for 15min; times, that is, the number of Bacillus coagulans is about 1×10 4 cfu / mL.
[0031] (3) Coating treatment: remove the scales, viscera, head and tail of the large yellow croaker, wash the slices, soak in the preservative in step (1) for 5 minutes, take it out and drain for 1 minute, put it on an iron rack and place it L...
Embodiment 3
[0034](1) Cultivation of Bacillus coagulans fermentation broth: First, pick activated Bacillus coagulans (preservation number: CCTCC NO: M 2013193) from the nutrient agar slant medium, inoculate it in the nutrient broth seed medium, 37°C Cultivate on a shaking table for 12 hours, inoculate the seed liquid in the nutrient broth medium at an inoculation amount of 6%, and culture at 37°C for 24 hours to obtain a fermentation liquid. The number of Bacillus coagulans in the fermentation broth was determined to be about 1×10 5 cfu / mL.
[0035] (2) Preparation of Bacillus coagulans suspension: centrifuge the obtained fermentation broth at 4°C, 8000rpm / min for 15min; times, that is, the number of Bacillus coagulans is about 1×10 3 cfu / mL.
[0036] (3) Coating treatment: remove the scales, viscera, head and tail of the large yellow croaker, wash the slices, soak in the preservative in step (1) for 5 minutes, take it out and drain for 1 minute, put it on an iron rack and place it Le...
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