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Composite microbiological bacteriocin saline-alkali soil improver, and preparation method and applications thereof

A technology of compound microbial bacteria and microbial agent, applied in application, fertilizer mixture, chemical instruments and methods, etc., can solve the problems of low organic matter content in saline-alkali soil, many harmful anions and cations, affecting crop rooting and promoting seedlings, etc., to achieve Rice seedlings are robust, promote rice rooting, and increase the number of effects

Active Publication Date: 2013-09-25
TSINGHUA UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Alkaline soil refers to the soil containing carbonate or heavy phosphate, the pH value is high, the soil is alkaline, the organic matter content of saline-alkali soil is small, the soil fertility is low, the physical and chemical properties are poor, and there are many anions and cations harmful to crops, which seriously affect Crop rooting promotes seedlings, resulting in low grain yield

Method used

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  • Composite microbiological bacteriocin saline-alkali soil improver, and preparation method and applications thereof
  • Composite microbiological bacteriocin saline-alkali soil improver, and preparation method and applications thereof
  • Composite microbiological bacteriocin saline-alkali soil improver, and preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] A compound microorganism bacteriocin saline-alkali soil modifier, the modifier includes microorganisms, organic liquids and substrates.

[0048] Further, the microorganisms include Bacillus subtilis (Bacillus subtilis) and Bacillus megaterium (Bacillus megaterium), and the weight ratio of the Bacillus subtilis and Bacillus megaterium is:

[0049] Bacillus subtilis 60~80

[0050] Bacillus megaterium 20-40;

[0051] The optimal weight ratio is:

[0052] Bacillus subtilis 70

[0053] Bacillus megaterium 30.

[0054] Further, the Bacillus subtilis is a mixture of Bacillus subtilis CGMCC7296, Bacillus subtilis CGMCC 7297 and Bacillus subtilis CGMCC 7298; the Bacillus megaterium is CGMCC 7337.

[0055] The organic liquid described in this example includes protease, lipase, α-amylase, and amino acids produced by the fermentation of the microbial inoculum.

[0056] The matrix described in the present embodiment comprises chicken manure or cow dung, lignite, phosphate rock po...

Embodiment 2

[0060] A preparation method of a compound microbial bacteriocin saline-alkali soil modifier, the manufacturing process steps of the modifier are as follows:

[0061] The compound microbial bacteriocin saline-alkali soil improver is obtained by fermentation of 3 strains of Bacillus subtilis and 1 strain of Bacillus megaterium;

[0062] The three strains of Bacillus subtilis are Bacillus subtilis CGMCC 7296, Bacillus subtilis CGMCC 7297, and Bacillus subtilis CGMCC 7298; the Bacillus megaterium is CGMCC 7337.

[0063] 1) strain preparation

[0064] The 3 strains of Bacillus asparticus and 1 strain of Bacillus megaterium were separately activated on the slanted surface of common broth to obtain activated strains; the activated strains were respectively transferred into flat bottles, and 28- Incubate at 30°C for 24h, and use 0.1 peptone sterile aqueous solution to prepare No. 1 seed suspension, No. 2 seed suspension, No. 3 seed suspension, and No. 4 seed suspension;

[0065] 2) ...

Embodiment 3

[0084] This embodiment is an improvement on the basis of Embodiment 2, and the same parts as those of Embodiment 2 will not be repeated.

[0085] The preparation method of the compound microbial bacteriocin saline-alkali soil modifier of the present embodiment, the manufacturing process steps of the modifier are as follows:

[0086] 2) Cultivation of seed pots

[0087] The formula of the culture medium in the A seed tank and the ratio of its parts by weight are:

[0088]

[0089]

[0090] The temperature of the A seed tank is 34°C, and the pH of the cultivation is 7.5;

[0091] The formula of the medium in the B seed tank and the ratio of parts by weight are:

[0092]

[0093] 3) Fermentation tank fermentation

[0094] The medium formula of the fermentor and its weight and number ratio are:

[0095]

[0096]

[0097] 4) Adsorbed on the substrate

[0098] The fermented bacterial liquid and the substrate are fully stirred evenly, at a rate of 20-30 L / m 3 Aft...

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Abstract

The invention relates to a composite microbiological bacteriocin saline-alkali soil improver, and a preparation method and applications thereof. The composite microbiological bacteriocin saline-alkali soil improver comprises a microbial agent, an organic liquid and a matrix. The microbial agent specifically comprises bacillus subtilis and bacillus megaterium, and nutrient substances of protease, lipase, alfa-amylase, amino acid and the like can be genereated by fermentation of the microbial bacteriocins; and the microbial agent can be used to adjust soil acidity-alkalinity, reduce saline-alkali toxicity, adjust rice root system microbial environment, accelerate transition of trace elements comprising nitrogen, phosphorus and potassium, and accelerate root growth, root increase, seedling development and seedling strengthening, and helps to lay a good foundation for increase of rice production.

Description

technical field [0001] The invention relates to the field of biological bacterial fertilizer technology and saline-alkali land treatment, in particular to a compound microbial bacteriocin saline-alkali land improver and a preparation method and application thereof. Background technique [0002] Ecological restoration and improvement of saline-alkali land is a project that benefits the country and the people. It can increase the area of ​​arable land, tap agricultural potential, ensure food production, and be more conducive to the development of the agricultural industry. It is conducive to expanding the area of ​​grain planting and ensuring the effective supply of grain. It has achieved good results in saline-alkali land treatment in Inner Mongolia, Jilin and other regions of China. There are also many patents in saline-alkali land treatment, such as: Chinese patent CN101481281A (application date: 2009 February 3, application number: 200910066498.0, applicant: Jilin Province...

Claims

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Application Information

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IPC IPC(8): C09K17/00C09K101/00
Inventor 陈昌和王楠刘嘉秦文斌刘国辉魏凌恺刘会欣
Owner TSINGHUA UNIV
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