Method for producing methyl parathion hydrolase by using marine bacterium LYG01
A technology of methyl parathion and methyl parathion emulsifiable concentrate, applied in the field of methyl parathion hydrolase produced by marine bacteria LYG01, can solve problems such as threats to human health, environmental pollution, and ecological damage
Inactive Publication Date: 2015-02-18
HUAIHAI INST OF TECH
View PDF2 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
However, the pesticide remains in the soil, water, air and agricultural products after use, causing serious environmental pollution and ecological damage, and posing a serious threat to human health
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0067] Embodiment 1, 1. a marine bacterium ( Aranicola sp.) LYG01 method for producing methyl parathion hydrolase, characterized in that the steps are as follows:
[0068] (1) Preparation of enzyme induction medium: Based on seawater LB medium, enzyme induction medium was prepared; its composition was as follows: 1% tryptone, 0.5% yeast extract, 1% NaCl, 0.1% methyl parathion EC , the mass content of emulsifiable oil in methyl parathion emulsifiable oil is 50%, 1000mL of old sea water; Weigh tryptone, yeast extract and NaCl respectively, dilute with 1000 mL of old sea water, pack into 500 mL triangular flasks, the liquid volume 100mL, sterilized at 121°C for 30min; filter parathion-methyl emulsifiable concentrate with a 0.22μm filter membrane, and add it before bacterial inoculation;
[0069] (2) Enzyme production culture: Inoculate marine bacteria LYG01 into the enzyme-inducing medium with a liquid volume of 100mL according to the inoculum amount of 2%, at a rotation speed...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
| Property | Measurement | Unit |
|---|---|---|
| molecular weight | aaaaa | aaaaa |
Login to View More
Abstract
The invention relates to a method for producing methyl parathion hydrolase by using a marine bacterium LYG01. The enzyme provided by the invention is intracellular enzyme. According to the invention, a crude enzyme liquid can be obtained after ultrasonic crushing and ultracentrifugation. Within a range of a temperature of 37-40 DEG C and a pH value of 6-8, the bacterium has high enzyme-producing capacity. Through (NH4)2SO4 salting impurity protein removing and MonoQ anion exchange column purification, primarily purified enzyme can be obtained. The enzyme has high activity within a range of a temperature of 35-45 DEG C and a pH value of 7.0-8.5. Metal ions K<+>, Fe<3+>, and Mg<2+>, and dithiothreitol DTT and ethylenediamine tetraacetic acid EDTA can promote the activity of the enzyme. The method provided by the invention has high operability. The produced enzyme has wider adaptation range than that of the bacterium. The enzyme has high activity under natural conditions. With the enzyme, a pesticide degradation rate approaches 90%. The enzyme has higher practical value.
Description
technical field [0001] The invention relates to a method for producing enzymes by marine bacteria, in particular to a method for producing methyl parathion hydrolase by marine bacteria LYG01. Background technique [0002] Methyl parathion is widely used in my country. This pesticide has a strong insecticidal effect on a variety of agricultural pests and is of great significance to improving crop yields. However, the pesticide remains in the soil, water, air and agricultural products after use, causing serious environmental pollution and ecological damage, and posing a serious threat to human health. [0003] Microorganisms can degrade organophosphorus pesticides such as methyl parathion, which is mainly due to their ability to produce methyl parathion hydrolase, which can hydrolyze methyl parathion to generate phosphorothioate and p-nitrophenol. The reported enzyme-producing strains mainly include Flavobacterium ATCC27551, Pseudomonas diminuta MG, and Orthomonas M6. Compar...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/16C12R1/01
Inventor 李福后王伟霞陈丽安贤惠秦蕾
Owner HUAIHAI INST OF TECH