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Isopimarane diterpenoid compounds and application thereof

A technology of isopimarane and compound, which is applied in the field of microorganisms to achieve obvious inhibitory effect

Inactive Publication Date: 2015-01-28
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there are few research reports on the isolation of isopimarane diterpenoids from the plant endophytic fungus-derived Pyramides

Method used

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  • Isopimarane diterpenoid compounds and application thereof
  • Isopimarane diterpenoid compounds and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The separation and preparation of compounds A, B and C:

[0020] 1. Slant culture of Xylaria sp. YM311647 strain: peel potatoes, cut into small pieces, add water to boil, filter with gauze to obtain potato filtrate, then add glucose and agar, use distilled water to make up volume, sterilize to obtain slant medium; Make the slant medium into a test tube slant, pick the YM311647 bacterial strain and insert it into the slant medium for cultivation to obtain the slant bacterial strain;

[0021] 2. Seed culture of Xanthomonas YM311647 strain: Take a strain graft block from the above slant strains and inoculate it into PDB medium (PDB medium: 200 g of peeled potatoes, 20 g of glucose, 15 g of agar, 1000 mL of distilled water , natural pH, sterilized at 121°C for 30 minutes), cultured on a shaker at 28°C and 200 r / min for 3 days to obtain seed liquid;

[0022] 3. The liquid fermentation culture of the Xylella cerevisiae YM311647 strain: 200 mL of PDB medium was placed in a...

Embodiment 2

[0025] The antibacterial activity of the isopimarane diterpene compound of the present invention, that is, compounds A, B and C, was detected by a micro-double dilution method.

[0026] 1. A total of five strains of pathogenic indicator bacteria: including 2 strains of human pathogenic fungi: Candida albicans ( Candida albicans ) and Cladomonospora ( Hormodendrum compactum ); 3 plant pathogenic fungi: Aspergillus niger ( Aspergillus niger ), Magnaporthe oryzae ( Pyricularia oryzae ) and Fusarium avenae ( Fusarium avenaceum ).

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Abstract

The invention relates to isopimarane diterpenoid compounds separated from fermentation products of Xylariasp.YM311647 and an application thereof, belonging to the technical field of microbes. The production strain YM311647 is collected in China Center for Type Culture Collection (CCTCC) on September 19, 2012, and the collection number is CCTCC No: M2012365. Three isopimarane diterpenoid compounds A, B and C are obtained by conventional fermentation, extraction and separation of the strain YM311647. Antifungal activity tests prove that the compounds have obvious antifungal activity and potential applications in preparation of new agricultural or medical antifungal medicaments.

Description

technical field [0001] The invention relates to three compounds, in particular to a class of isopimarane diterpene compounds with antibacterial activity derived from Xylaria sp. YM311647 (Xylaria sp. YM311647) and applications thereof, belonging to the technical field of microorganisms. Background technique [0002] Plant endophytic fungi have rich diversity and are a large class of biological resources with little development. They can produce new active compounds of various structural types and are important sources of active secondary products. They have become one of the research hotspots at home and abroad. , especially for endophytic fungi from temperate and tropical plants. Neem ( Azadirachta indica A. Juss) is a tropical tree species with high biopesticide and medicinal value. We isolated an endophytic fungus from neem plant: Xylaria sp. YM311647 (Xylaria sp. YM311647). [0003] Li et al. isolated Xylarenolide from the fruiting body of Xylaria sp. 101 derived from...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D307/77C07C305/16C07J73/00C12P17/04C12P15/00C12P33/20A01P3/00A61P31/10C12R1/645
Inventor 吴少华陈有为苗翠苹
Owner YUNNAN UNIV
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