Galactomyces geotrichum P14, application method thereof, and microbial inoculum prepared from same

An application method, the technology of phosphate-dissolving bacteria agent, applied in the direction of application, chemical instruments and methods, and methods based on microorganisms, can solve the problem of fewer strains, reduce the amount of phosphate fertilizer, improve the utilization rate of phosphate fertilizer, and promote the development of crop roots. Effect

Active Publication Date: 2013-10-23
HUNAN INST OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although dissolved (dissolved) phosphorus microbial fertilizers have entered into commercial prod...

Method used

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  • Galactomyces geotrichum P14, application method thereof, and microbial inoculum prepared from same
  • Galactomyces geotrichum P14, application method thereof, and microbial inoculum prepared from same
  • Galactomyces geotrichum P14, application method thereof, and microbial inoculum prepared from same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Preparation of embodiment 1 Geotrichum candidum strain P14 and its phosphate-dissolving bacterial agent

[0043] 1. Composition of medium in fermenter (g / L)

[0044] Soluble starch 10g / L; corn flour 5g / L; yeast powder 5g / L; sucrose 10g / L; (NH 4 ) 2 SO 4 0.5g / L; KCl0.3g / L; NaCl0.3g / L; KH 2 PO 4 0.1g / L; MgSO 4 ·7H 2 O0.3g / L; FeSO 4 0.03g / L; MnSO 4 ·4H 2 O0.03g / L; add water to 1L; pH7.0~7.4.

[0045] The composition of the liquid medium in the triangular flask: peeled potatoes 150g / L, corn flour 10g / L, glucose 20g / L, KH 2 PO 4 0.5g / L, MgSO 4 ·7H 2 O0.5g / L; add water to 1L; pH natural.

[0046] The composition of the medium in the seed tank: yeast powder 0.5g / L; glucose 10.0g / L; KH 2 PO 4 1.0g / L; (NH 4 ) 2 SO 4 0.5g / L; NaCl0.2g / L; MgSO 4 ·7H 2 O0.03g / L; MnSO 4 0.03g / L; FeSO 4 0.003g / L; add water to make up to 1L; pH7.0~7.4.

[0047] 2. Training process

[0048] Inoculate Geotrichum phospholyticum P14 strain on potato comprehensive medium (PDA), cult...

Embodiment 2

[0049] The phosphorus-dissolving ability of embodiment 2 Geotrichum candidum P14

[0050] 1. Phosphorus dissolving effect under solid culture conditions

[0051] In the selective medium - modified Pikovskaya medium (medium composition (g / L): (NH 4 ) 2 SO 4 0.5, NaCl 0.2, MgSO 4 ·7H 2 O 0.03, KCl 0.2, MnSO 4 0.03, FeSO 4 0.003, yeast powder 0.5, glucose 10.0, phosphorus source 5.0) —— On the top, the soil sample collected in the red soil phosphorus ore surrounding the phosphorus ore surrounding the Liuyang phosphorus ore in Changsha will be diluted step by step. -4 The plate was coated and the phosphorus-dissolving (dissolving) microorganisms were separated. According to the size of the phosphorus-dissolving circle, 30 strains of phosphorus-dissolving bacteria and fungi were preliminarily isolated. Then, on the selective medium with tricalcium phosphate as the only phosphorus source, the phosphorus-dissolving ability of these phosphorus-dissolving (solubilizing) bact...

Embodiment 3

[0064] Example 3 Utilization of Exogenous Refractory Phosphorus by Geotrichum candidum P14

[0065] Under pot conditions, using light loamy fluvo-aquic soil and corn as the test crop, the effect of Geotrichum candidum P14 on the bioavailability of exogenous insoluble phosphorus was studied. The insoluble phosphorus used in the test included tricalcium phosphate, octaphosphate Calcium, iron phosphate, aluminum phosphate, bone meal, fluoroapatite, and Moroccan phosphate rock were used as phosphorus sources for testing, and the phosphorus-dissolving effects are shown in Table 5.

[0066] Table 5 Variation of soil available phosphorus over time with different phosphorus source treatments

[0067]

[0068] Under the conditions of this experiment, the results showed that Geotrichum phosphate-solubilizing P14 had a negative effect on Ca 3 (PO 4 ) 2 , AlPO 4 , MPR has a strong activation ability for FePO 4 The activation effect was weak, showing that Geotrichum phosphate-solubil...

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Abstract

The invention provides galactomyces geotrichum P14, an application method thereof, and a microbial inoculum prepared from the same, which belong to the technical field of micro-biofertilizer, and aim to realize high-efficiency phosphorus solubilization from the biological aspect by virtue of screened phosphorus solubilizing fungus. According to the microbial inoculum prepared from the galactomyces geotrichum P14, various insoluble phosphates in soil are converted to be in a water-soluble state with high efficiency, and then can be absorbed and utilized by plants. The microbial inoculum is suitable for reddened soil, moisture soil, brown soil and other types of soil, and has the effects of releasing insoluble phosphorus in the soil, preventing soil chemical fixation of phosphorus, improving the utilization efficiency of phosphorus fertilizer, improving the agronomic characters of crops, and increasing yield.

Description

technical field [0001] The invention belongs to the field of soil microbial bacterial agents, and in particular relates to a strain of Geotrichum geotrichum P14 (Galactomyces geotrichum P14) and the preparation and application of a phosphorus-dissolving bacterial agent containing the bacteria. Background technique [0002] At present, the demand for phosphorus fertilizer in agricultural production is increasing day by day. According to statistics, the area of ​​phosphorus deficiency in my country accounts for about 2 / 3 of the total cultivated land area. The main method is to rely on the application of chemical phosphate fertilizers, but it has caused soil deterioration, decreased fertility, soil compaction, soil acidification, and soil pollution by harmful substances. In particular, the results of the "First National Survey of Pollution Sources" show that the degree of damage to the natural environment by agriculture has reached a historical high, mainly due to the excessive...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N11/14C09K17/00C12R1/645C09K101/00
Inventor 吴迎奔贺月林陈薇许丽娟尹红梅王震刘标杜东霞张爱群陈海荣丁祥力
Owner HUNAN INST OF MICROBIOLOGY
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