Strain for producing L-lysine and method thereof for producing L-lysine

Abstract

The invention relates to a strain for producing L-lysine, which is classified and named as Escherichiacoli NT1003 delta Met delta Thr and is collected in China Center for Type Culture Collection (CCTCC) on May 30, 2013, and the collection number is CCTCC NO: M2013239. The invention further provides a mutation screening method of the strain and a method for producing the L-lysine by utilizing the strain. After continuous passage is performed on the Escherichiacoli NT1003 delta Met delta Thr strain for 7 times, the content of the L-lysine in a fermentation solution is about 60g / L and has no significant change, so that the genetic stability is good.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an L-lysine-producing bacterial strain and a method for producing L-lysine. Background technique [0002] L-Lysine (L-Lysine, Lys) is one of the essential amino acids for organisms to maintain life activities. It is the second largest amino acid species in the world after monosodium glutamate, and it is also an important precursor for new materials such as bio-based nylon. The production methods of L-lysine include extraction method, chemical synthesis / enzyme method and microbial fermentation method. Although the traditional extraction process is simple, the source of raw materials such as animal blood meal is limited, which is limited to small-scale production; the production of lysine by the synthetic method requires the use of highly toxic raw material phosgene, and there may be residual catalysts, which leads to poor product safety and serious environmental problems ; The chemic...

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Problems solved by technology

Although the traditional extraction process is simple, the source of raw materials such as animal blood meal is limited, which is limited to small-scale production; the production of lysine by the synthetic method requires the use of highly toxic raw material phosgene, and there may be residual catalysts, which leads to poor product safety and serious environmental problems ; The chemically synthesized DL-lysine also needs to be split or converted by enzymatic methods to obtain L-lysine, which greatly increases the production cost; microbial fermentation is the most important method for industrial production of lysine at present. Widely available and inexpensive, such as starch (tapioca, cornstarch, etc.), molasses (cane molasses, beet molasses, etc.)

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