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Method for detecting bisphenol A derivatives in drink with LC-MS-MS (liquid chromatography-mass spectrometry-mass spectrometry)

A technology of LC-MS-MS and derivatives, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of poor sensitivity, long detection time, complicated detection methods, etc. good effect

Active Publication Date: 2015-04-29
ORG TECH CO LTD
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the methods for detecting bisphenol A and bisphenol A derivatives mainly include LC-MS method, LC-FL method and LC-MS-MS method, etc. However, there are detection limit deviations in LC-MS method and LC-FL method. High, poor sensitivity, complex detection methods, and long detection time; and LC-MS-MS method is limited to the detection of bisphenol A, but there is no record of simultaneous detection of multiple bisphenol A derivatives

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  • Method for detecting bisphenol A derivatives in drink with LC-MS-MS (liquid chromatography-mass spectrometry-mass spectrometry)
  • Method for detecting bisphenol A derivatives in drink with LC-MS-MS (liquid chromatography-mass spectrometry-mass spectrometry)
  • Method for detecting bisphenol A derivatives in drink with LC-MS-MS (liquid chromatography-mass spectrometry-mass spectrometry)

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Effect test

Embodiment 1

[0072] After freezing and centrifuging the carbonated beverage matrix at a temperature of -12°C and a centrifugal rate of 10,000 rad / min, add mobile phase to dilute to 10 times the volume of the original carbonated beverage matrix, filter through a filter membrane with a pore size of 0.45 μm, and perform LC - MS-MS detection.

[0073] Table 5 is the detection result of the spiked recovery rate diluted 10 times in the carbonated beverage matrix.

[0074] The sample preparation method is as follows: respectively take BADGE, BADGE.H with a concentration of 1ppm 2 O. BADGE.2H 2 O, BADGE.HCl, BADGE.2HCl, BADGE.HCl.H 2 O, BFDGE, BFDGE.2H 2 O. Each 100 μL of 9 kinds of standard solutions of BFDGE.2HCl was added to 2100 μL of carbonated beverage matrix, and the mixed solution was ultrasonically mixed evenly. , and carried out 6 parallel LC-MS-MS detections, the results are shown in Table 5 below:

[0075] Table 5 carbonated beverage matrix spiked recovery

[0076]

[0077] It...

Embodiment 2

[0079] After freezing and centrifuging the drinking mineral water matrix at a temperature of -12°C and a centrifugal rate of 10,000 rad / min, add mobile phase to dilute it to 10 times the volume of the original drinking mineral water matrix, and filter it through a filter membrane with a pore size of 0.45 μm. Perform LC-MS-MS detection.

[0080] Table 6 is the detection result of the recovery rate of standard addition of drinking mineral water base dilution 10 times.

[0081] The sample preparation method is as follows: respectively take BADGE, BADGE.H with a concentration of 1ppm 2 O. BADGE.2H 2 O, BADGE.HCl, BADGE.2HCl, BADGE.HCl.H 2 O, BFDGE, BFDGE.2H 2 O. Each 100 μL of 9 standard solutions of BFDGE.2HCl was added to 2100 μL of drinking mineral water matrix, and the mixed solution was ultrasonically mixed evenly. The actual concentration of the mixed standard solution was 33.3ppb, and it was processed according to the above pretreatment method Afterwards, 6 parallel LC-...

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Abstract

The invention provides a method for detecting bisphenol A derivatives in a drink with LC-MS-MS (liquid chromatography-mass spectrometry-mass spectrometry). The method comprises the following steps of: (1) determining LC-MS-MS conditions; (2) detecting standard liquid of the bisphenol A derivatives of a target detection object according to the LC-MS-MS conditions and drawing a standard curve of the corresponding bisphenol A derivatives according to a detection result; (3) carrying out sample pretreatments including refrigerated centrifugation, dilution and filtering on the drink, detecting according to the LC-MS-MS conditions, comparing the detection result with the standard curve and calculating the contents of the corresponding bisphenol A derivatives in the sample. The detection method is high in sensitivity and accuracy, short in detection time, good in recovery test effect and suitable for standardization. By utilizing the method, nine or more bisphenol A derivatives can be detected simultaneously.

Description

technical field [0001] The invention relates to a method for detecting bisphenol A derivatives in beverages, in particular to a method for quantitatively detecting bisphenol A derivatives in beverages by LC-MS-MS. Background technique [0002] Bisphenol A (Bisphenol A, BPA) is one of the most widely used industrial compounds in the world, mainly used in the production of polycarbonate, epoxy resin, polysulfone resin, polyphenylene ether resin, unsaturated polyester resin, etc. Polymer Materials. Discussions about bisphenol A have been in European and American countries for a long time, until March 22, 2010, the Danish government confirmed the research results published by the Danish University of Science and Technology-National Food Research Institute (DTUFood)-bisphenol A will affect the nervous system and activities ability, all countries have a unified understanding of the impact of bisphenol A. The European Union believes that bisphenol A can be precipitated into food ...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 赵宇晖曾科宋莉华柏建国赵彦
Owner ORG TECH CO LTD
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