Triptolide liposome preparation for treatment of small cell lung cancer and preparation method thereof

A technology of triptolide and liposome preparation, which is applied in the field of traditional Chinese medicine, can solve the problems of low cancer curative effect, unsatisfactory curative effect, large toxic and side effects, etc., and achieve the effect of improving clinical curative effect

Inactive Publication Date: 2013-11-20
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemotherapeutic drugs have a certain effect on cancer, but they have severe side effects

Method used

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  • Triptolide liposome preparation for treatment of small cell lung cancer and preparation method thereof
  • Triptolide liposome preparation for treatment of small cell lung cancer and preparation method thereof
  • Triptolide liposome preparation for treatment of small cell lung cancer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: A triptolide liposome preparation for the treatment of small cell lung cancer is made of triptolide, and the preparation method of its liposome is as follows:

[0026] (1) The ratio of raw materials for preparing liposomes is: lecithin 900mg, amphipathic polyethylene glycol 2000-distearoylphosphatidylethanolamine 90mg, cholesterol 90mg;

[0027] (2) The preparation method of triptolide liposomes is prepared by ethanol injection method: take 90ml of PBS buffer solution with pH 7.4 in an eggplant-shaped bottle, and preheat it in a constant temperature water bath at 50°C for 35min;

[0028] (3) Take the liposome proportioned in step 1 in a beaker, add 36 mg of triptolide, and then add an appropriate amount of absolute ethanol to dissolve it;

[0029] (4) After the triptolide is completely dissolved, quickly inject it into the eggplant-shaped bottle with a buffer solution preheated at a constant temperature in step 2 to obtain a milky white liposome suspension...

Embodiment 2

[0030] Embodiment 2: A triptolide liposome preparation for the treatment of small cell lung cancer is made of triptolide, and the preparation method of its liposome is as follows:

[0031] (1) The ratio of raw materials for preparing liposomes is: lecithin 600mg, amphiphilic polyethylene glycol 2000-distearoylphosphatidylethanolamine 60mg, cholesterol 60mg;

[0032] (2) The preparation method of triptolide liposomes is prepared by ethanol injection method: take 90ml of PBS buffer solution with pH 7.4 in an eggplant-shaped bottle, and preheat it in a constant temperature water bath at 55°C for 30min;

[0033] (3) Take the liposome proportioned in step 1 in a beaker, add 24mg of triptolide, and then add an appropriate amount of absolute ethanol to dissolve it;

[0034] (4) After triptolide is completely dissolved, quickly inject it into the eggplant-shaped bottle with buffer solution preheated at constant temperature in step 2 to obtain a milky white liposome suspension, continu...

Embodiment 3

[0035] Example 3: Inhibitory effect of a triptolide liposome preparation on transplanted small cell lung cancer in nude mice

[0036] 1. Experimental animals and tumor strains

[0037] BALB / C-nu nude mice, 50, male and female, 4-6 weeks old, weighing 18-20 g, were purchased from the Animal Experiment Center of our school. Human small cell lung cancer (SCLC) cell line NCI-H446 was purchased from the Animal Experiment Center of Jiangsu Cancer Institute.

[0038] 2. Modeling and experimental methods

[0039] Of 50 BALB / C-nu nude mice, 10 were randomly selected as the negative control group, and the remaining 40 were used for modeling. Take human small cell lung cancer NCI-H446 cells in logarithmic growth phase cultured in vitro, and adjust the cell concentration to 1×10 7 / ml. Under sterile conditions, human small cell lung cancer NCI-H446 cells were inoculated on the back of BALB / C-nu nude mice with an inoculation volume of 0.2ml / mouse (the number of cells was 2×10 6 / Only)...

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Abstract

The invention discloses a triptolide liposome preparation for treatment of small cell lung cancer (SCLC) and a preparation method thereof. The preparation is a liposome preparation prepared from a Chinese medicinal active ingredient triptolide for treating small cell lung cancer. According to the preparation method, an ethanol injection method is employed to prepare the liposome, thus obtaining the triptolide liposome preparation for treatment of small cell lung cancer provided in the invention. Animal in-vivo anticancer pharmacological experiments show that the triptolide liposome preparation has an obvious inhibitory effect on small cell lung cancer growth, and its anticancer activity is significantly higher than that of a chemotherapeutic drug cisplatin. The anticancer effect of the triptolide liposome preparation is higher than that of triptolide non-liposome preparations.

Description

1. Technical field [0001] The invention relates to the technical field of traditional Chinese medicine, in particular to a triptolide liposome preparation for treating small cell lung cancer and a preparation method thereof. 2. Background technology [0002] Cancer is a formidable enemy that endangers human health. There are about 1.6 million new cancer cases in my country every year, and about 1.3 million people die of cancer every year. The number of deaths due to cancer in the country is increasing year by year. Not high enough. [0003] There are currently no effective drugs that cure most cancer patients. Although chemotherapeutic drugs have certain curative effects on cancer, they have severe side effects, and Chinese patent medicines generally have low curative effects on cancer, and the curative effects are not ideal. [0004] The present inventor has studied the anticancer effective substances of traditional Chinese medicines such as tiger eye evergreen, yew, toad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K31/585A61P35/00A61P11/00
Inventor 章永红章迅
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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