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Application of miR-23a as acute aortic dissection differential diagnosis marker and acute aortic dissection diagnosis reagent kit

A technology of mir-23a and aortic dissection, applied in the direction of DNA / RNA fragments, recombinant DNA technology, etc., can solve the problems of lack of specificity and short half-life, and achieve the effect of strong specificity, low price and high sensitivity

Active Publication Date: 2015-07-01
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The academic community has done a lot of research to find biomarkers of acute aortic dissection, and found that such as: C-reactive protein, D-dimer, smooth muscle myosin heavy chain, calponin, etc. may be related to the occurrence of acute aortic dissection A series of associated biomarkers have not been widely used in the clinical diagnosis and treatment of acute aortic dissection due to factors such as lack of specificity, short half-life, or limitation of false lumen thrombosis

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  • Application of miR-23a as acute aortic dissection differential diagnosis marker and acute aortic dissection diagnosis reagent kit
  • Application of miR-23a as acute aortic dissection differential diagnosis marker and acute aortic dissection diagnosis reagent kit
  • Application of miR-23a as acute aortic dissection differential diagnosis marker and acute aortic dissection diagnosis reagent kit

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Embodiment Construction

[0016] The specific embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0017] In the specific implementation process of the present invention, the reagents used in the corresponding steps are as follows: RNA extraction is carried out with the miRNeasy Mini Kit purchased from Qiagen, which includes 2ml centrifuge tube (without cover), 1.5ml centrifuge tube (with cover), QIAzol lysis Reagent, RNA / DNase-free water, RWT buffer, RPE buffer, and Rneasy Mini spin column filter column; purchased from Applied Biosystems MicroRNA Reverse Transcription Kit (Catalog No. 4366597) for RNA reverse transcription, including: 10×Reverse Transcription Buffer, 100mM dNTPs(with dTTP), 20U / μl Rnase Inhibitor, 50U / μl MultiScribeTM Reverse Transcriptase; purchased from Applied Biosystems Universal PCR Master Mix II, no UNG (Cat. No. 4440048) for real-time quantitative PCR. In addition, RNA reverse transcription primers and real-t...

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Abstract

The invention provides an application of miR-23a as an acute aortic dissection differential diagnosis marker, wherein the sequence of the miR-23a is as shown in SEQ ID NO.1. An acute aortic dissection diagnosis reagent kit is characterized by being provided with a total plasma RNA (Ribose Nucleic Acid) extracting reagent group, an external reference, an RNA reverse transcription reagent group, an RNA reverse transcription primer group, a real-time quantitative PCR (Polymerase Chain Reaction) reagent group, a real-time quantitative PCR primer group and a real-time quantitative PCR probe group, wherein the RNA reverse transcription primer group comprises a miR-23a reverse transcription primer and an external reference reverse transcription primer; the real-time quantitative PCR primer group comprises a miR-23a real-time quantitative PCR primer and an external reference real-time quantitative PCR primer; and the real-time quantitative PCR probe group comprises a miR-23a real-time quantitative PCR probe and an external reference real-time quantitative PCR probe.

Description

technical field [0001] The invention relates to the technical field of biological detection. Background technique [0002] Acute aortic dissection (AAD) refers to the high-speed and high-pressure blood flow in the aorta entering the media layer or the junction of media and adventitia through the intima tear on the aortic wall, thereby forming a true and false aortic cavity. And expand along the long axis of the aorta, which can lead to aortic rupture leading to death of the patient or acute ischemia of important organs (such as: heart, brain, kidney, intestinal tract, etc.) causing severe complications. one of the serious diseases. In our clinical practice, we found that many patients with acute aortic dissection died of diseased aortic rupture before surgical treatment or even a definite diagnosis. According to investigations and studies, the mortality rate of patients with untreated Stanford type A acute aortic dissection is as high as 1% to 2% / h at the onset of the dise...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 景在平周建董健陆清声赵志青包俊敏冯睿冯翔袁良喜
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY