Liquid protein chip kit for detecting liver fibrosis degree

Abstract

The invention provides a liquid protein chip kit for detecting a liver fibrosis degree. In the liquid protein chip kit, coupling antibody microspheres comprise 1, fluorescent microspheres coupling with procollagen peptide III and / or a metalloprotease tissue inhibitor factor-1 capture antibody, 2, one or more of fluorescent microspheres coupling with collagen peptide IV, fibronectin, a transforming growth factor-beta 1, a platelet-derived growth factor, a tumor necrosis factor alpha capture antibody, and an angiotensin II capture antibody, and 3, fluorescent microspheres coupling with a serum hyaluronic acid capture antibody and / or a laminin capture antibody. The liquid protein chip kit can realize combined detection of a plurality of liver fibrosis-related markers for reflection of different liver fibrosis stages, can shorten detection time to more than ten minutes from a few hours, can finish detection of a plurality of markers only by 1 microliter of a serum sample, can be operated simply and has a low diagnosis cost.

Description

Technical field [0001] The invention relates to an in vitro detection kit, in particular to a liquid protein chip kit for detecting the degree of liver fibrosis, a preparation method thereof, and a method for non-invasive detection of liver fibrosis-related markers. Background technique [0002] Liver Fibrosis is the common pathological basis of various chronic liver diseases and the final common path of liver damage. Chronic liver diseases caused by different causes such as various viruses, immune and toxic substances, and metabolic damage, if they are not diagnosed and treated in time, can gradually develop into liver fibrosis, and eventually develop into liver cirrhosis, portal hypertension, liver cancer and Liver failure. It is now believed that liver fibrosis is a dynamic process with potential cure, so early diagnosis of liver fibrosis is particularly important. [0003] At present, there are many methods for diagnosing liver fibrosis reported at home and abroad, mainly his...

AI Technical Summary

Problems solved by technology

[0005] Although the separate detection and joint analysis of markers can reflect the degree of liver fibrosis to varying degrees, each marker requires a radiation or enzyme-linked immunosorbent kit, so these multi-index detection methods have the required amount of serum. Large size, long detection time, high diagnostic cost, complex operation, difficult joint analysis, etc.

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