Liquid protein chip kit for detecting liver fibrosis degree

A protein chip and liver fibrosis technology, applied in biological testing, material inspection products, fluorescence/phosphorescence, etc., can solve the problems of high diagnostic cost, large amount of serum consumption, long detection time, etc., to eliminate subjective errors, serum samples The effect of small amount and low diagnosis cost

Inactive Publication Date: 2013-11-20
SHANGHAI TONGJI HOSPITAL
View PDF7 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the separate detection and joint analysis of markers can reflect the degree of liver fibrosis to varying degrees, each marker requires a radiation or enzyme-linked immunosor

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Liquid protein chip kit for detecting liver fibrosis degree
  • Liquid protein chip kit for detecting liver fibrosis degree
  • Liquid protein chip kit for detecting liver fibrosis degree

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0042] Hereinafter, the present invention will be further described with reference to the drawings and specific embodiments to better understand the present invention.

[0043] 1. Preparation of a liquid protein chip kit for detecting the degree of liver fibrosis

[0044] 1. Screening of serological markers

[0045] The formation of liver fibrosis involves pathogenic body reactions, reactive cells, cytokines, overexpression of extracellular matrix (ECM), increased collagen synthase activity, and decreased degrading enzyme activity. The present invention preferably adopts the following 10 serological markers with high sensitivity and relatively good specificity:

[0046] Markers reflecting early liver fibrosis: type III procollagen peptide (PIIIP), tissue inhibitor of metalloproteinase-1 (TIMP-1);

[0047] Markers reflecting the active phase: type IV collagen peptide (PIV), fibronectin (FN), transforming growth factor-β1 (TGF-βl), platelet-derived growth factor (PDGF-BB), tumor necrosis...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a liquid protein chip kit for detecting a liver fibrosis degree. In the liquid protein chip kit, coupling antibody microspheres comprise 1, fluorescent microspheres coupling with procollagen peptide III and/or a metalloprotease tissue inhibitor factor-1 capture antibody, 2, one or more of fluorescent microspheres coupling with collagen peptide IV, fibronectin, a transforming growth factor-beta 1, a platelet-derived growth factor, a tumor necrosis factor alpha capture antibody, and an angiotensin II capture antibody, and 3, fluorescent microspheres coupling with a serum hyaluronic acid capture antibody and/or a laminin capture antibody. The liquid protein chip kit can realize combined detection of a plurality of liver fibrosis-related markers for reflection of different liver fibrosis stages, can shorten detection time to more than ten minutes from a few hours, can finish detection of a plurality of markers only by 1 microliter of a serum sample, can be operated simply and has a low diagnosis cost.

Description

Technical field [0001] The invention relates to an in vitro detection kit, in particular to a liquid protein chip kit for detecting the degree of liver fibrosis, a preparation method thereof, and a method for non-invasive detection of liver fibrosis-related markers. Background technique [0002] Liver Fibrosis is the common pathological basis of various chronic liver diseases and the final common path of liver damage. Chronic liver diseases caused by different causes such as various viruses, immune and toxic substances, and metabolic damage, if they are not diagnosed and treated in time, can gradually develop into liver fibrosis, and eventually develop into liver cirrhosis, portal hypertension, liver cancer and Liver failure. It is now believed that liver fibrosis is a dynamic process with potential cure, so early diagnosis of liver fibrosis is particularly important. [0003] At present, there are many methods for diagnosing liver fibrosis reported at home and abroad, mainly his...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/68G01N21/64
Inventor 杨长青杨丽
Owner SHANGHAI TONGJI HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap